Detailed information NTG0686990

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M3I83_RS20655
• Genome accession: NZ_CP097079
• Coordinates: 4261760..4262560 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain KP131
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4256760..4267560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M3I83_RS20620 (M3I83_20605)	gspH	4256916..4257431 (+)	516	WP_020804560.1	type II secretion system minor pseudopilin GspH	-
M3I83_RS20625 (M3I83_20610)	gspI	4257428..4257793 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M3I83_RS20630 (M3I83_20615)	gspJ	4257790..4258386 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
M3I83_RS20635 (M3I83_20620)	gspK	4258383..4259369 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
M3I83_RS20640 (M3I83_20625)	gspL	4259356..4260549 (+)	1194	WP_020804558.1	type II secretion system protein GspL	-
M3I83_RS20645 (M3I83_20630)	-	4260542..4261027 (+)	486	WP_004145896.1	type II secretion system protein M	-
M3I83_RS20650 (M3I83_20635)	-	4261017..4261763 (+)	747	WP_004145897.1	type II secretion system protein N	-
M3I83_RS20655 (M3I83_20640)	pilD	4261760..4262560 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
M3I83_RS20660 (M3I83_20645)	hrpB	4262608..4265037 (-)	2430	WP_020804570.1	ATP-dependent helicase HrpB	-
M3I83_RS20665 (M3I83_20650)	thpR	4265110..4265646 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
M3I83_RS20670 (M3I83_20655)	sfsA	4265646..4266362 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M3I83_RS20675 (M3I83_20660)	dksA	4266525..4266980 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=686990 M3I83_RS20655 WP_004213441.1 4261760..4262560(+) (pilD) [Klebsiella pneumoniae strain KP131]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=686990 M3I83_RS20655 WP_004213441.1 4261760..4262560(+) (pilD) [Klebsiella pneumoniae strain KP131]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425