Detailed information NTG0686966

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M3I84_RS20660
• Genome accession: NZ_CP097076
• Coordinates: 4261741..4262541 (+)
• Length: 266 a.a.
• NCBI ID: WP_004213441.1
• Uniprot ID: A0A9Q4U0T4
• Organism: Klebsiella pneumoniae strain KP133
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4256741..4267541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M3I84_RS20625 (M3I84_20605)	gspH	4256897..4257412 (+)	516	WP_020804560.1	type II secretion system minor pseudopilin GspH	-
M3I84_RS20630 (M3I84_20610)	gspI	4257409..4257774 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M3I84_RS20635 (M3I84_20615)	gspJ	4257771..4258367 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
M3I84_RS20640 (M3I84_20620)	gspK	4258364..4259350 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
M3I84_RS20645 (M3I84_20625)	gspL	4259337..4260530 (+)	1194	WP_020804558.1	type II secretion system protein GspL	-
M3I84_RS20650 (M3I84_20630)	-	4260523..4261008 (+)	486	WP_004145896.1	type II secretion system protein M	-
M3I84_RS20655 (M3I84_20635)	-	4260998..4261744 (+)	747	WP_004145897.1	type II secretion system protein N	-
M3I84_RS20660 (M3I84_20640)	pilD	4261741..4262541 (+)	801	WP_004213441.1	prepilin peptidase	Machinery gene
M3I84_RS20665 (M3I84_20645)	hrpB	4262589..4265018 (-)	2430	WP_020804570.1	ATP-dependent helicase HrpB	-
M3I84_RS20670 (M3I84_20650)	thpR	4265091..4265627 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
M3I84_RS20675 (M3I84_20655)	sfsA	4265627..4266343 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M3I84_RS20680 (M3I84_20660)	dksA	4266506..4266961 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29405.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=686966 M3I84_RS20660 WP_004213441.1 4261741..4262541(+) (pilD) [Klebsiella pneumoniae strain KP133]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=686966 M3I84_RS20660 WP_004213441.1 4261741..4262541(+) (pilD) [Klebsiella pneumoniae strain KP133]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425