Detailed information NTG0685688

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: M2I99_RS02175
• Genome accession: NZ_CP096960
• Coordinates: 474786..475934 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain NY11254
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 469786..480934

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2I99_RS02150 (M2I99_02150)	-	470937..471233 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
M2I99_RS02155 (M2I99_02155)	-	471230..471823 (-)	594	WP_003084544.1	YggT family protein	-
M2I99_RS02160 (M2I99_02160)	proC	471834..472655 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
M2I99_RS02165 (M2I99_02165)	-	472667..473359 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
M2I99_RS02170 (M2I99_02170)	pilT	473574..474608 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
M2I99_RS02175 (M2I99_02175)	pilU	474786..475934 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
M2I99_RS02180 (M2I99_02180)	czcD	475942..476841 (-)	900	WP_003084557.1	CDF family zinc transporter CzcD	-
M2I99_RS02185 (M2I99_02185)	-	476996..477400 (+)	405	WP_003084560.1	NINE protein	-
M2I99_RS02190 (M2I99_02190)	-	477643..479016 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
M2I99_RS02195 (M2I99_02195)	-	479013..480197 (+)	1185	WP_003084564.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=685688 M2I99_RS02175 WP_003100959.1 474786..475934(+) (pilU) [Pseudomonas aeruginosa strain NY11254]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=685688 M2I99_RS02175 WP_003100959.1 474786..475934(+) (pilU) [Pseudomonas aeruginosa strain NY11254]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACTCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382