Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   M2I84_RS26300 Genome accession   NZ_CP096932
Coordinates   5538383..5539255 (+) Length   290 a.a.
NCBI ID   WP_003125204.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NY5510     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5533383..5544255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2I84_RS26275 (M2I84_26210) - 5534252..5534494 (+) 243 WP_023109353.1 hypothetical protein -
  M2I84_RS26285 (M2I84_26220) pilA 5534771..5535223 (-) 453 WP_028681203.1 pilin Machinery gene
  M2I84_RS26290 (M2I84_26225) pilB 5535455..5537155 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M2I84_RS26295 (M2I84_26230) pilC 5537159..5538379 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  M2I84_RS26300 (M2I84_26235) pilD 5538383..5539255 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  M2I84_RS26305 (M2I84_26240) coaE 5539252..5539863 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  M2I84_RS26310 (M2I84_26245) yacG 5539860..5540060 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  M2I84_RS26315 (M2I84_26250) - 5540097..5540306 (-) 210 WP_003094660.1 hypothetical protein -
  M2I84_RS26320 (M2I84_26255) - 5540412..5541101 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  M2I84_RS26325 (M2I84_26260) - 5541098..5541568 (-) 471 WP_003094664.1 hypothetical protein -
  M2I84_RS26330 (M2I84_26265) - 5541565..5541990 (-) 426 WP_003116252.1 GNAT family N-acetyltransferase -
  M2I84_RS26335 (M2I84_26270) - 5542123..5542752 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  M2I84_RS26340 (M2I84_26275) - 5542749..5543198 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  M2I84_RS26345 (M2I84_26280) - 5543224..5543394 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31839.89 Da        Isoelectric Point: 7.7068

>NTDB_id=685333 M2I84_RS26300 WP_003125204.1 5538383..5539255(+) (pilD) [Pseudomonas aeruginosa strain NY5510]
MPLLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=685333 M2I84_RS26300 WP_003125204.1 5538383..5539255(+) (pilD) [Pseudomonas aeruginosa strain NY5510]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
TTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.714

96.552

0.538
  pilD Vibrio campbellii strain DS40M4

53.004

97.586

0.517
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

49.281

95.862

0.472