Detailed information NTG0685165

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M2J85_RS03620
• Genome accession: NZ_CP096920
• Coordinates: 788795..789661 (-)
• Length: 288 a.a.
• NCBI ID: WP_003247536.1
• Uniprot ID: -
• Organism: Pseudomonas putida strain NY4811
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 783795..794661

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M2J85_RS03585 (M2J85_03575)	-	784269..785564 (-)	1296	WP_003247549.1	NAD(P)/FAD-dependent oxidoreductase	-
M2J85_RS03590 (M2J85_03580)	-	785649..785822 (-)	174	WP_008091951.1	DUF3094 domain-containing protein	-
M2J85_RS03595 (M2J85_03585)	-	785955..786584 (-)	630	WP_003247546.1	DUF1780 domain-containing protein	-
M2J85_RS03600 (M2J85_03590)	-	786640..787071 (-)	432	WP_060538607.1	hypothetical protein	-
M2J85_RS03605 (M2J85_03595)	-	787158..787865 (+)	708	WP_060538606.1	energy-coupling factor ABC transporter permease	-
M2J85_RS03610 (M2J85_03600)	yacG	787975..788175 (-)	201	WP_003247540.1	DNA gyrase inhibitor YacG	-
M2J85_RS03615 (M2J85_03605)	coaE	788172..788795 (-)	624	WP_004575721.1	dephospho-CoA kinase	-
M2J85_RS03620 (M2J85_03610)	pilD	788795..789661 (-)	867	WP_003247536.1	prepilin peptidase	Machinery gene
M2J85_RS03625 (M2J85_03615)	-	789664..790872 (-)	1209	WP_054888521.1	type II secretion system F family protein	-
M2J85_RS03630 (M2J85_03620)	-	791089..791499 (+)	411	WP_012051131.1	pilin	-
M2J85_RS03640 (M2J85_03630)	-	791869..793269 (+)	1401	WP_060538560.1	PAAR domain-containing protein	-
M2J85_RS03645 (M2J85_03635)	-	793262..793651 (+)	390	WP_060538559.1	hypothetical protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31808.87 Da
• Isoelectric Point: 7.9101

>NTDB_id=685165 M2J85_RS03620 WP_003247536.1 788795..789661(-) (pilD) [Pseudomonas putida strain NY4811]
MTLWALLAEQPAYFLTLATVLGLLVGSFINVLVYRLPIMLERQWQREAQEVLGLPTTQHPRFDLCLPASRCPHCAHRIRA
WENIPVISYLALGGRCSSCKNRISLRYPVVEVASALLSLVVAWRFGASVEALLALPLTWCLLALSLIDADHQLLPDVLVL
PTMWLGLIVNAFGIHVPLADALWGAVAGYLSLWTVYWVFRLVTGKEGMGYGDFKLMALIGAWGGWQVLPLTLLLSSVVGA
LVGLCLLRFRRHALGTAIPFGPYLAIAGWIAVLWGDEIYASYIQLLGY

Nucleotide

• Length: 867 bp

>NTDB_id=685165 M2J85_RS03620 WP_003247536.1 788795..789661(-) (pilD) [Pseudomonas putida strain NY4811]
ATGACTTTATGGGCTTTGCTGGCTGAACAGCCAGCGTATTTCCTCACCCTTGCCACGGTGCTCGGCCTGCTGGTGGGCAG
CTTTATCAACGTGCTGGTGTATCGCCTGCCGATCATGCTGGAGCGGCAATGGCAGCGTGAGGCGCAAGAGGTATTGGGGC
TGCCGACCACGCAGCACCCGCGTTTCGACCTGTGCCTGCCCGCCTCCCGGTGCCCACACTGCGCGCACCGGATTCGTGCG
TGGGAGAACATTCCGGTCATCAGCTACCTGGCCTTGGGCGGGCGCTGTTCGTCCTGCAAAAACCGCATCAGCCTGCGTTA
CCCGGTAGTGGAAGTGGCCAGCGCGCTGCTGTCGCTGGTAGTGGCCTGGCGCTTCGGCGCTTCGGTAGAGGCGTTGCTGG
CGTTGCCGCTGACCTGGTGCCTGCTGGCGCTGAGCCTGATCGATGCGGACCACCAGCTGCTGCCGGATGTGCTGGTGCTG
CCGACCATGTGGCTAGGGCTGATCGTCAATGCCTTTGGCATCCATGTGCCCCTGGCCGACGCACTGTGGGGCGCAGTGGC
CGGGTACCTCAGCCTGTGGACGGTGTACTGGGTGTTCAGGCTGGTCACCGGCAAGGAAGGCATGGGCTATGGCGATTTCA
AGCTGATGGCGCTGATCGGGGCGTGGGGGGGTTGGCAGGTGCTGCCGTTGACGCTGCTGTTGTCGTCGGTGGTGGGGGCG
CTTGTTGGCCTGTGCTTGCTGCGTTTTCGCCGGCATGCCTTGGGCACGGCTATCCCGTTTGGCCCTTATCTGGCAATTGC
GGGGTGGATTGCCGTGCTCTGGGGTGATGAAATATACGCCTCATACATACAACTGCTTGGATACTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-125)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.986	96.181	0.5
pilD	Acinetobacter baumannii D1279779	49.11	97.569	0.479
pilD	Acinetobacter nosocomialis M2	48.399	97.569	0.472
pilD	Vibrio campbellii strain DS40M4	49.091	95.486	0.469
pilD	Neisseria gonorrhoeae MS11	48.327	93.403	0.451