Detailed information NTG0068491

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: JP49775_RS07760
• Genome accession: NZ_AP017922
• Coordinates: 1581615..1582112 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain JP02758
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1576615..1587112

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JP49775_RS07735 (JP02758_1470)	gcvPB	1576777..1578249 (-)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
JP49775_RS07740 (JP02758_1471)	gcvPA	1578242..1579588 (-)	1347	WP_029549656.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
JP49775_RS07745 (JP02758_1472)	gcvT	1579608..1580699 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
JP49775_RS07750 (JP02758_1473)	-	1580858..1581382 (-)	525	WP_029549655.1	shikimate kinase	-
JP49775_RS07755	-	1581372..1581518 (-)	147	WP_001789879.1	hypothetical protein	-
JP49775_RS07760 (JP02758_1474)	comGF	1581615..1582112 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
JP49775_RS07765 (JP02758_1475)	comGE	1582030..1582329 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
JP49775_RS07770 (JP02758_1476)	comGD	1582316..1582762 (-)	447	WP_001791207.1	competence type IV pilus minor pilin ComGD	Machinery gene
JP49775_RS07775 (JP02758_1477)	comGC	1582740..1583051 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
JP49775_RS07780 (JP02758_1478)	comGB	1583065..1584135 (-)	1071	WP_000776422.1	competence type IV pilus assembly protein ComGB	Machinery gene
JP49775_RS07785 (JP02758_1479)	comGA	1584107..1585081 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
JP49775_RS07790 (JP02758_1480)	-	1585133..1585756 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
JP49775_RS07795 (JP02758_1481)	-	1585753..1586082 (-)	330	WP_029549654.1	MTH1187 family thiamine-binding protein	-
JP49775_RS07800 (JP02758_1482)	-	1586082..1587068 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=68491 JP49775_RS07760 WP_001825340.1 1581615..1582112(-) (comGF) [Staphylococcus aureus strain JP02758]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=68491 JP49775_RS07760 WP_001825340.1 1581615..1582112(-) (comGF) [Staphylococcus aureus strain JP02758]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97