Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EFK13_RS05995 Genome accession   NZ_CP096889
Coordinates   1170237..1173941 (+) Length   1234 a.a.
NCBI ID   WP_129506144.1    Uniprot ID   -
Organism   Bacillus cabrialesii strain TE3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1165237..1178941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFK13_RS05980 (EFK13_05980) - 1165695..1166021 (-) 327 WP_129506147.1 four-helix bundle copper-binding protein -
  EFK13_RS05985 (EFK13_05985) - 1166138..1166575 (-) 438 WP_129506146.1 ferritin family protein -
  EFK13_RS05990 (EFK13_05990) addB 1166750..1170250 (+) 3501 WP_129506145.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EFK13_RS05995 (EFK13_05995) addA 1170237..1173941 (+) 3705 WP_129506144.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EFK13_RS06000 (EFK13_06000) sbcD 1174014..1175189 (+) 1176 WP_129506143.1 exonuclease subunit SbcD -
  EFK13_RS06005 (EFK13_06005) sbcC 1175186..1178581 (+) 3396 WP_129506142.1 exonuclease subunit SbcC -
  EFK13_RS06010 (EFK13_06010) hlpB 1178596..1178898 (+) 303 WP_129506141.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141186.62 Da        Isoelectric Point: 5.1234

>NTDB_id=684858 EFK13_RS05995 WP_129506144.1 1170237..1173941(+) (addA) [Bacillus cabrialesii strain TE3]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYARGDKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLLRA
LELTKAPGGPAPRADNFLDDLAQIDELIQQQDDFYELYKRVPAVSFKRAKAVKGDEFDAALLDEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILAAENDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDELAELKLGAAYPDNDETETELLLIGSSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVTLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQAFYGHLQKWRSFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMETLAENSAAAHADISGHPARFAVRMIHSYD
LLDDDLEERMEEKSERLEAIRQGEPVPGSFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHAPTIEEAERTVQKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
QLIGAKWKEREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYK
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=684858 EFK13_RS05995 WP_129506144.1 1170237..1173941(+) (addA) [Bacillus cabrialesii strain TE3]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCGGGCTCCGGGAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGATCGGCTGCTCGTTGTGACATTTACAAACGCCTCGGCGGCAGAAATGAAACACCGAATCGCGGATGCC
CTGGAAAAAGAACTTGTAAAGCGCCCCGGCTCGTTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCACTCCTTTTGCCTGCAAGTGCTGAAAAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCCG
ATCAGACAGAAGGCGAGCTGCTTGGGGATGAAGTGCTGGATGAGCTGTTTGAAGATGAATACGCAAGGGGCGACAAGGCG
TTTTTTGAACTTGTTGACCGCTATACGACAGACCGGCACGATTTGGATCTGCAATTTCTTGTTAAACAGGTGTACGAGTA
TTCCCGATCCCATCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATACGATGTATCCGAAAAGAGCGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAAGAAAAGCTCCTGCGCGCG
CTTGAGCTGACGAAAGCGCCGGGCGGCCCCGCGCCGCGCGCTGATAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCAGCAGGACGATTTTTATGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAGGCAGTAAAAG
GGGACGAGTTTGATGCTGCGTTGCTTGATGAAGCAACGGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTCAAA
ACTGACTATTTCACGAGAAGTCCTGAGCAGCACTTGAAAAGCTTAGCCGACATGAAGCCTGTCATTGAGACGCTCGTGCA
GCTTGTCATCAGCTACGGAAAACGGTTTGAAGCGGCGAAACAGGAAAAATCAATCATCGATTTTTCTGATTTGGAGCATT
ACTGCTTGTCCATTTTGGCAGCTGAAAATGACAAAGGTGAACGCGAGCCGAGTGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGATGAATATCAAGATACAAACCTCGTGCAGGAATCGATCCTGCAGCTTGTCACAAGCGGGCC
GGAGGAGACTGGAAACCTGTTTATGGTAGGGGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTCCTTT
TCCTTTCCAAATACAAACGGTTTACAGAAAGCGGAGAAGGCACAGGACGGAAAATCGACTTGAATAAAAATTTCCGTAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCTGGCTGAACTGAAGCTCGGTGCGGCATATCCGGACAATGATGAGACGGAAACGGAGCTGCTGCTGATCGGCAGCTCAG
AAGAGACGGATGCAGGTGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAATTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGCTCCATGCCGTGGGCGCCGCAAATCATGGAAGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCACGCTCTCAGTGCTGAAGGTGATCGATAACCCTTACCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCTTACTATGAAGCGATGAAAGACTACTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAGCGT
TCTACGGACATCTGCAAAAATGGCGCTCGTTTTCAAAAAACCACTCAGTATCTGAACTGATTTGGGAAGTGTACCGCGAC
ACGAAGTACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTCCTTTACGACCGGGC
GCGCCAATATGAAGCGACGGCATTTCGCGGCCTGTTCCGTTTTCTGCGGTTTATCGAACGCATGCAGGAGCGAGGGGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATTCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTACGGCGGGTCTCGGCCGCAATTTCAACATGATGGACTTGAACAAATCGTATCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTTTATTCATCCGCAATTGCGCATCAGCTATCCGACGCTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAACTGCTGTCAGAGGAATTGCGTGTTCTGTATGTTGCATTAACGAGAGCAAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCGAAATGGCAGGCATCCGCGTCACAAACTGACTGGCTTCTGCCTGA
ATTTGACCGCTATCAGGCAAAAACGTATTTAGATTTCATCGGGCCGGCCCTTGCCAGACACAGAGATATGGAGACTTTGG
CTGAAAACAGTGCGGCTGCGCATGCTGACATCTCGGGACACCCGGCTCGTTTTGCCGTCCGAATGATCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGAATGGAAGAAAAAAGCGAACGCTTAGAAGCGATTCGCCAAGGTGAACCAGTTCC
CGGCTCGTTTGCGTTTGATGAAAAAGCCCGTGAGCAGCTGAGCTGGACATATCCGCATCACGAGGTGACGCAAATTCGCA
CAAAGCAGTCGGTGTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGTTAAACCGGCT
GACGGAAGCATTCTGTACAGGCGTCCGGCTTTCATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGAACGGCCATGCA
TACGGTGATGCAGCATATCCCGCTGACACATGCGCCGACGATAGAAGAAGCGGAGCGGACGGTTCAAAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTTCAATTCTTCCACACGGAAATCGGCGGA
CAGCTGATCGGCGCGAAATGGAAGGAGCGGGAAGTGCCATTCAGTTTGGCGCTTCCGGCCAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATTGACTGTCTCTATGAAACTGAGGACGGTTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGCTTTGAAGGAGCGGCCCCGATTTTGAAAAAACGATATAAA
ACGCAAATTCAGCTGTACACGAAGGCTGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

95.948

100

0.959