Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   EFK13_RS05990 Genome accession   NZ_CP096889
Coordinates   1166750..1170250 (+) Length   1166 a.a.
NCBI ID   WP_129506145.1    Uniprot ID   -
Organism   Bacillus cabrialesii strain TE3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1161750..1175250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFK13_RS05970 (EFK13_05970) - 1162610..1163812 (-) 1203 WP_129506149.1 MFS transporter -
  EFK13_RS05975 (EFK13_05975) - 1163926..1165656 (+) 1731 WP_129506148.1 ABC transporter substrate-binding protein -
  EFK13_RS05980 (EFK13_05980) - 1165695..1166021 (-) 327 WP_129506147.1 four-helix bundle copper-binding protein -
  EFK13_RS05985 (EFK13_05985) - 1166138..1166575 (-) 438 WP_129506146.1 ferritin family protein -
  EFK13_RS05990 (EFK13_05990) addB 1166750..1170250 (+) 3501 WP_129506145.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EFK13_RS05995 (EFK13_05995) addA 1170237..1173941 (+) 3705 WP_129506144.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EFK13_RS06000 (EFK13_06000) sbcD 1174014..1175189 (+) 1176 WP_129506143.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134815.81 Da        Isoelectric Point: 5.4755

>NTDB_id=684857 EFK13_RS05990 WP_129506145.1 1166750..1170250(+) (addB) [Bacillus cabrialesii strain TE3]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLSEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDMTYKELNGTERHLQAPELAHLEAQYEARPAIPYSEKQDAFTVMQAANR
RAELEGIAREIHSLVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFRLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVMQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRIYGTSCTFVLGANDGVVPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVIKRLEELFPQHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSEDWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=684857 EFK13_RS05990 WP_129506145.1 1166750..1170250(+) (addB) [Bacillus cabrialesii strain TE3]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAGCTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTTCTCCAGCACACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGTGTACAGATGCTCCTGCGGAAACTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTAGAACGGATGCTGACAGAGTTTAAGCGCTACT
GTCTGGAACCGGAAGATATCCGCAGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTGTTTTATCTGAA
AAGCTTCATGACTTATCGATTCTCTATCAGCAGATGGAAAAAAGCCTCGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCGGAGCATATTCCGCTGTCGGAGGATGTAAAGGGCGCGCATATCTATGTGGACGGCTTTTATCAGTTTA
CACCGCAGGAGTTCAGGGTGTTGGAGCAGCTTATGGTTCACGCAGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATACGAGCGGGAGCCACATGAACTGGAATTGTTCAGAATGACTGGAAAAACTTATTACCGTCTGCATCAGAAGGCGAA
GGAACTGAATCTGGACATGACCTATAAAGAGCTAAACGGGACTGAGCGGCACTTGCAGGCGCCGGAACTCGCACATTTAG
AGGCGCAGTATGAAGCGCGTCCGGCCATCCCATACTCAGAAAAACAAGATGCCTTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTCGAAGGCATTGCCCGGGAAATTCACTCTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGACTACAAAGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCATCCGTTAATCGAATTTATCCGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGCATTAAAGGCGACCGCTGGACAAAGGGCGACCGTTTCCATTACAGGCGTT
TTGTGTCATTGGATGATGATTTTGCGCAGACCGACCAGGAAATCGAAATGGAAAACATGCTGAATGACACTCGCGACTGG
ATAGTTCCGCCGCTTTTTCGGCTCCAGAAACGAATGAAAAAAGCAAAGACGGTTCAGGAAAAGGCAGAGGCGCTCTATCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAAGAAGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGATGCGGTTATGCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTCATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTATCCAGAATATATGGCACCTCTTGCACCTTTGTGCTGGGGGCAAATGACGGCGTTG
TGCCGGCGCGCCCTGATGAAAACGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGAGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGACGAGCATTTCCTCATTTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCAGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCATTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAAGAGCGCTTGTTGACAAATGAACCTGAGCAGGTCAGCGATGAGGAACAGCTTATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCCAGCCAGCTCAGATTATGGACACGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGCGAGCCGGACAGGCTGCAATCGAAAAAGCTGTTTTCAAGCCTGTTTTTCCGTAATGAAGTAAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGCGAACGTATTCAGGGCAGTGTGTCGAGAATGGAAACCTTTAACGCG
TGCCCGTTCTCCCATTTTGCATCACACGGGCTGCACCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGTCTGCGGGAGCAAAAAATGGATTGGCGCGATTTGACGAAAG
AGCAGTGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTACGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTGCCGATCGGACTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAGCTCGTCGGGCGAATTGACCGCGTCGATAAAGCGGAAAGCTCAAAAGGCCTGCTTCTGAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGACTCGCGCTCCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGAAGACTGGCTCGGAATGAGAGCGACTCCGGCCGGGGTGCTGTACTTCCATATTCATGACC
CAATGATTCAATCTAATCTTCCGCTTGGACTTGACGAGATTGAACAGGAAATTTTTAAGAAATTTAAAATGAAGGGCCTG
CTCCTTGGCGATCAAGAAGCGGTTCGCCTCATGGATACGACCCTTCAAGAGGGACGGTCAAATATCATCAATGCCGGCTT
GAAAAAAGACGGCTCTCTCAGATCAGATTCAGCGGCGGTCGGCGAAAAGGAATTTGAGCTGTTGACGAAGCATGTGCGCC
GCACCTTCCAAGAAGCAGGCGAACAAATCACCGACGGACGCGTATCCATTGAGCCTTATAAAATGAAGAACAAGACGCCG
TGCACATACTGTGCGTTCAAATCAGTATGTCAGTTTGATGAATCATTAGAAGAAAACGAGTATCGCCCGTTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(305-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.341

100

0.973