Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EFK13_RS00585 Genome accession   NZ_CP096889
Coordinates   106995..108371 (+) Length   458 a.a.
NCBI ID   WP_129506944.1    Uniprot ID   -
Organism   Bacillus cabrialesii strain TE3     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 101995..113371
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFK13_RS00565 (EFK13_00565) ctsR 102345..102809 (+) 465 WP_064814468.1 transcriptional regulator CtsR -
  EFK13_RS00570 (EFK13_00570) mcsA 102823..103383 (+) 561 WP_064814469.1 protein-arginine kinase activator protein McsA -
  EFK13_RS00575 (EFK13_00575) - 103383..104474 (+) 1092 WP_064814470.1 protein arginine kinase -
  EFK13_RS00580 (EFK13_00580) clpC 104471..106903 (+) 2433 WP_075747654.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  EFK13_RS00585 (EFK13_00585) radA 106995..108371 (+) 1377 WP_129506944.1 DNA repair protein RadA Machinery gene
  EFK13_RS00590 (EFK13_00590) disA 108375..109457 (+) 1083 WP_075747656.1 DNA integrity scanning diadenylate cyclase DisA -
  EFK13_RS00595 (EFK13_00595) - 109573..110673 (+) 1101 WP_129506943.1 PIN/TRAM domain-containing protein -
  EFK13_RS00600 (EFK13_00600) ispD 110688..111386 (+) 699 WP_129506942.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  EFK13_RS00605 (EFK13_00605) ispF 111379..111855 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49497.96 Da        Isoelectric Point: 7.6838

>NTDB_id=684842 EFK13_RS00585 WP_129506944.1 106995..108371(+) (radA) [Bacillus cabrialesii strain TE3]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIEEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=684842 EFK13_RS00585 WP_129506944.1 106995..108371(+) (radA) [Bacillus cabrialesii strain TE3]
ATGGCTAAAACAAAATCGAAATTTATCTGCCAATCCTGCGGTTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCGCCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACCGTACAGAAACCTTCACCGATTACATCAATCGAAACATCAGAAGAACCCCGGGTGAAAACCCAGCTCGGCGAATTT
AACAGAGTGCTCGGCGGAGGCGTCGTCAAAGGCTCACTTGTTTTAATTGGCGGCGATCCCGGTATCGGAAAATCAACGTT
ATTACTGCAGGTTTCCGCTCAATTGTCAGATTCATCGAACAGTGTGCTGTACATTTCAGGAGAGGAATCAGTGAAACAAA
CGAAACTGAGAGCAGACCGTCTTGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATAGAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATCACATCGGC
CCCGGGCAGTGTGTCACAGGTCAGAGAATGTACGGCTGAGCTGATGAAAATTGCAAAAACAAAAGGCATACCGATTTTTA
TCGTAGGGCACGTGACCAAAGAAGGGTCTATTGCAGGGCCGAGACTGTTGGAGCATATGGTTGATACTGTTTTATATTTT
GAAGGAGAACGTCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTTGGCTCCACAAATGAAATGGGCATTTT
TGAAATGCGCGAAGAGGGGCTTACTGAGGTTTTGAATCCTTCAGAAATTTTCTTAGAAGAACGTTCGGCGGGATCCGCAG
GCTCCAGCATAACTGCTTCTATGGAAGGTACAAGGCCGATTCTCGTTGAAATCCAGGCGCTCATCTCGCCAACAAGTTTT
GGCAATCCGCGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCACTGTTAATGGCTGTGTTAGAAAAAAGAGTGGG
GCTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCCGGCGGCGTTAAACTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATCAGTATCGCATCAAGCTTTAGAGATACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCTAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATCGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

AAA domain (AAA_25)

(72-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.127

100

0.991
  radA Streptococcus pneumoniae Rx1

63.576

98.908

0.629
  radA Streptococcus pneumoniae D39

63.576

98.908

0.629
  radA Streptococcus pneumoniae R6

63.576

98.908

0.629
  radA Streptococcus pneumoniae TIGR4

63.576

98.908

0.629
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629
  radA Streptococcus mitis SK321

63.355

98.908

0.627