Detailed information NTG0684376

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M1R63_RS03500
• Genome accession: NZ_CP096814
• Coordinates: 730499..731299 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Mu1413
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 725499..736299

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1R63_RS03480 (M1R63_03480)	dksA	726079..726534 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
M1R63_RS03485 (M1R63_03485)	sfsA	726697..727413 (-)	717	WP_020324420.1	DNA/RNA nuclease SfsA	-
M1R63_RS03490 (M1R63_03490)	thpR	727413..727949 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
M1R63_RS03495 (M1R63_03495)	hrpB	728022..730451 (+)	2430	WP_004177388.1	ATP-dependent helicase HrpB	-
M1R63_RS03500 (M1R63_03500)	pilD	730499..731299 (-)	801	WP_004145898.1	A24 family peptidase	Machinery gene
M1R63_RS03505 (M1R63_03505)	-	731296..732042 (-)	747	WP_004178605.1	type II secretion system protein N	-
M1R63_RS03510 (M1R63_03510)	-	732032..732517 (-)	486	WP_004145896.1	type II secretion system protein M	-
M1R63_RS03515 (M1R63_03515)	gspL	732510..733703 (-)	1194	WP_004194919.1	type II secretion system protein GspL	-
M1R63_RS03520 (M1R63_03520)	gspK	733690..734676 (-)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
M1R63_RS03525 (M1R63_03525)	gspJ	734673..735269 (-)	597	WP_004178612.1	type II secretion system minor pseudopilin GspJ	-
M1R63_RS03530 (M1R63_03530)	gspI	735266..735631 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
M1R63_RS03535 (M1R63_03535)	gspH	735628..736143 (-)	516	WP_004194939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=684376 M1R63_RS03500 WP_004145898.1 730499..731299(-) (pilD) [Klebsiella pneumoniae strain Mu1413]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=684376 M1R63_RS03500 WP_004145898.1 730499..731299(-) (pilD) [Klebsiella pneumoniae strain Mu1413]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCATGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425