Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   M1M80_RS06010 Genome accession   NZ_CP096592
Coordinates   1254444..1258148 (+) Length   1234 a.a.
NCBI ID   WP_060398328.1    Uniprot ID   -
Organism   Bacillus inaquosorum strain A65.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1249444..1263148
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M1M80_RS05995 (M1M80_05995) - 1249891..1250220 (-) 330 WP_060398326.1 four-helix bundle copper-binding protein -
  M1M80_RS06000 (M1M80_06000) - 1250337..1250774 (-) 438 WP_044427329.1 ferritin family protein -
  M1M80_RS06005 (M1M80_06005) addB 1250957..1254457 (+) 3501 WP_060398327.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  M1M80_RS06010 (M1M80_06010) addA 1254444..1258148 (+) 3705 WP_060398328.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  M1M80_RS06015 (M1M80_06015) sbcD 1258220..1259395 (+) 1176 WP_060398329.1 exonuclease subunit SbcD -
  M1M80_RS06020 (M1M80_06020) sbcC 1259392..1262784 (+) 3393 WP_060398330.1 exonuclease subunit SbcC -
  M1M80_RS06025 (M1M80_06025) hlpB 1262798..1263100 (+) 303 WP_060398331.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141273.54 Da        Isoelectric Point: 5.0440

>NTDB_id=682090 M1M80_RS06010 WP_060398328.1 1254444..1258148(+) (addA) [Bacillus inaquosorum strain A65.1]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIAYA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKEKLQRA
LELTKAPGGPAPRADNFLDDLAQIDELIQHQDDFTELYKRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
TDYFTRNPEQHLKSLADMKPVIETLIQLVISYGNRFEAAKQEKSIIDFSDLEHYCLSILTAENDKGEQEPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAIALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQATASQTDWLLPEFDRYQAKTYLDFIGPALARHRDLEALAENGAGAHADISGHPARFAVQMIHSYD
LLDDDLEERLEEKSERLEAIRRGEPVPGPFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVHKLYEKELLTEEQKAAIDIEEIVQFFHTEIGG
RLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTQVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=682090 M1M80_RS06010 WP_060398328.1 1254444..1258148(+) (addA) [Bacillus inaquosorum strain A65.1]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
TGTGGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATTACCGCAGAAGAAAACC
CGATTGATGTAGACCGGCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACATCGGATCGCGTATGCC
CTGGAAAAAGAGCTTGTAAAGCGCCCCGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAAGTGCTGGACGAGCTGTTTGAAGACGAATACGCGAAGGGTGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGATCGGCACGATTTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCTCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCGGAAAAGAGCGCAATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCGATGGTGCTTAACGGAGCGAAGGAGAAGCTGCAGCGCGCG
CTTGAACTGACGAAAGCGCCGGGCGGACCGGCGCCGCGCGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGACGATTTCACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GTGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAATGGCGCAAAAAAACTTCTCGAAAAGCTCAAA
ACCGACTACTTTACGAGAAACCCTGAACAGCATTTGAAAAGCTTAGCCGACATGAAGCCTGTCATTGAAACACTCATACA
GCTTGTCATCAGCTACGGAAACCGATTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCAGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAACGACAAAGGCGAACAGGAGCCGAGCGAGGCAGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTACAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
GGAAGAGACCGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCAGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGACGGAAAATCGACTTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTTTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTACCCGGACAATGATGAGACGGAAACAGAGCTGCTGCTGATCGACAACTCAG
AAGAGACGGATGCAGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCTAAGGAAATTCGT
AAGTTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGTTACGTTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTCGCGATCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTTTGCGCTCACCGATTGTCGGGGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGCAAACGT
TCTACGGACATCTGCAAAAATGGCGTGCGTTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAT
ACAAAATACATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGCGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTTCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTGTTTGCAGCGGGTCTCGGACGGAACTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTCGGGTTTGGCACGAAGTTTATTCATCCGCAATTGCGCATCAGCTATCCGACGCTTCCGCTCATTGCCATGAAGAAAA
AAATGCGCAGGGAGCTATTGTCAGAGGAATTGCGTGTTCTCTATGTCGCATTAACGAGAGCGAAGGAAAAGCTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCAACTGCGTCCCAAACTGACTGGCTTCTCCCGGA
ATTTGACCGCTATCAGGCAAAAACCTATTTAGATTTCATCGGGCCGGCTCTTGCCAGACACAGAGATTTGGAGGCTTTGG
CTGAAAACGGTGCGGGAGCGCATGCTGACATATCAGGCCACCCGGCTCGTTTTGCCGTTCAAATGATCCATTCCTATGAT
TTGCTGGATGATGATCTGGAAGAAAGATTGGAAGAAAAAAGCGAGCGCCTAGAAGCGATCCGCCGAGGTGAACCAGTTCC
CGGCCCGTTTGCGTTTGATGAGAAAGCCCGGGAGCAGCTAAGCTGGACATATCCGCATCATGAGGTGACGCAAATTCGCA
CAAAGCAGTCAGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCAGGGCCCCCGTTAAACCGGCA
GATGGAAGCATTCTGTACAGGCGTCCCGCTTTTATGATGAAAAAAGGTTTGACAGCGGCGGAAAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTCACCCATGTGCCGACAATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAGCTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTTCAATTTTTCCATACAGAAATCGGCGGA
CGGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTCAGTTTAGCGCTTCCGGCTAAGGAGATCTATCCTGATGC
ACAGGAGGCGGATGAGCCGCTTTTAGTGCAGGGAATCATCGACTGTCTCTATGAAACTGAGGACGGTTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCAGCACGGTTTTGAAGGAGCGGCCCCGATCTTGAAAAAACGATACGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACACAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.677

100

0.967