Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M0696_RS12265 Genome accession   NZ_CP096590
Coordinates   2442563..2443633 (-) Length   356 a.a.
NCBI ID   WP_166850818.1    Uniprot ID   -
Organism   Bacillus rugosus strain A78.1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2437563..2448633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0696_RS12220 (M0696_12220) tapA 2438045..2438803 (-) 759 WP_248601999.1 amyloid fiber anchoring/assembly protein TapA -
  M0696_RS12225 (M0696_12225) - 2439074..2439400 (+) 327 WP_222143412.1 YqzG/YhdC family protein -
  M0696_RS12230 (M0696_12230) - 2439443..2439622 (-) 180 WP_166850851.1 YqzE family protein -
  M0696_RS12235 (M0696_12235) comGG 2439693..2440067 (-) 375 WP_166850848.1 competence type IV pilus minor pilin ComGG Machinery gene
  M0696_RS12240 (M0696_12240) comGF 2440068..2440451 (-) 384 WP_248602000.1 competence type IV pilus minor pilin ComGF Machinery gene
  M0696_RS12245 (M0696_12245) comGE 2440477..2440824 (-) 348 WP_166850846.1 competence type IV pilus minor pilin ComGE Machinery gene
  M0696_RS12250 (M0696_12250) comGD 2440808..2441239 (-) 432 WP_166850843.1 competence type IV pilus minor pilin ComGD Machinery gene
  M0696_RS12255 (M0696_12255) comGC 2441229..2441525 (-) 297 WP_166850840.1 comG operon protein ComGC Machinery gene
  M0696_RS12260 (M0696_12260) comGB 2441539..2442576 (-) 1038 WP_222143154.1 competence type IV pilus assembly protein ComGB Machinery gene
  M0696_RS12265 (M0696_12265) comGA 2442563..2443633 (-) 1071 WP_166850818.1 competence protein ComGA Machinery gene
  M0696_RS12270 (M0696_12270) - 2443846..2444256 (-) 411 WP_222143155.1 CBS domain-containing protein -
  M0696_RS12275 (M0696_12275) - 2444319..2445266 (-) 948 WP_222143156.1 magnesium transporter CorA family protein -
  M0696_RS12280 (M0696_12280) - 2445410..2446738 (+) 1329 WP_222143157.1 hemolysin family protein -
  M0696_RS12285 (M0696_12285) - 2446790..2447629 (-) 840 WP_166850806.1 STAS domain-containing protein -
  M0696_RS12295 (M0696_12295) mgsR 2447860..2448237 (-) 378 WP_003226302.1 transcriptional regulator MgsR -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40566.84 Da        Isoelectric Point: 9.4577

>NTDB_id=681965 M0696_RS12265 WP_166850818.1 2442563..2443633(-) (comGA) [Bacillus rugosus strain A78.1]
MDSIEKISRTLIKEAYITKASDIHIVPRERDAIIHFRVDHALLKKRNMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLEEGNVHLRMSTLPTINEESLVIRVMPQYHIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGVNMNEIEQTVIAIAAQRLVDLACPFCENRCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=681965 M0696_RS12265 WP_166850818.1 2442563..2443633(-) (comGA) [Bacillus rugosus strain A78.1]
TTGGATTCAATAGAAAAGATAAGCAGAACCTTGATTAAAGAGGCATATATAACAAAGGCTTCTGATATTCACATTGTACC
GAGGGAGCGGGACGCCATCATTCATTTTCGGGTTGATCATGCCCTACTGAAAAAAAGGAACATGAAAAAAGAAGAGTGCG
TAAGATTGATTTCACATTTTAAATTTCTCTCAGCGATGGATATAGGGGAAAGGCGAAAGCCACAAAATGGGTCGCTTACG
CTAAAGCTTGAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTCCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCTCAATACCATATCCCTTCGATTGATAAACTGTCGTTATTTCCGAAGACTGGAGCCACTTTACTATCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACCACCACATTATACTCTCTTGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACACTGGAGGATCCTGTTGAAACAAGGGACGAGGATGTTCTTCAAGTGCA
GGTGAATGAAAAAGCCGGTGTGACATATTCCGCAGGGCTGAAGGCTATTTTGCGTCATGACCCGGATATGATTATTTTAG
GTGAGATTAGAGACGCGGAAACAGCTAAAATTGCGGTTCGGGCAGCGATGACGGGGCATCTGGTGCTGACGAGCCTTCAT
ACGAGAGATGCGAAGGGTGCGATTTACAGACTGCTTGAATTCGGTGTGAATATGAATGAAATCGAACAGACTGTGATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACAGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAACACCAGGAGAGCAAGCGTGTATGAGCTCCTTTACGGAAAAAATCTTCAACAATGTATTCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTACGTCAAATTATCAGAAAAGGAATTGCACTCGGCTACTTGACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.966
  pilB Glaesserella parasuis strain SC1401

41.801

87.36

0.365