Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   M0696_RS05915 Genome accession   NZ_CP096590
Coordinates   1172974..1176678 (+) Length   1234 a.a.
NCBI ID   WP_248602592.1    Uniprot ID   -
Organism   Bacillus rugosus strain A78.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1167974..1181678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0696_RS05900 (M0696_05900) - 1168467..1168772 (-) 306 Protein_1098 four-helix bundle copper-binding protein -
  M0696_RS05905 (M0696_05905) - 1168866..1169303 (-) 438 WP_222142561.1 ferritin family protein -
  M0696_RS05910 (M0696_05910) addB 1169487..1172987 (+) 3501 WP_222142562.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  M0696_RS05915 (M0696_05915) addA 1172974..1176678 (+) 3705 WP_248602592.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  M0696_RS05920 (M0696_05920) sbcD 1176750..1177925 (+) 1176 WP_166853692.1 exonuclease subunit SbcD -
  M0696_RS05925 (M0696_05925) sbcC 1177922..1181314 (+) 3393 WP_248602593.1 exonuclease subunit SbcC -
  M0696_RS05930 (M0696_05930) hlpB 1181328..1181630 (+) 303 WP_166853690.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141309.60 Da        Isoelectric Point: 4.9776

>NTDB_id=681937 M0696_RS05915 WP_248602592.1 1172974..1176678(+) (addA) [Bacillus rugosus strain A78.1]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSNPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIAMVLNGAKENLQRA
LELTKAPGGPAPRADTFLDDLAQIDELIQYQDDFNELYRRVPAVSFKRAKAVKGDEFDPALLEEATDLRNGAKKLLEKLK
SDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENDKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNTEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENTKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGSKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENGAAAHADISGHPARFAVRMIHSYD
LLDDDLEERMEEKSERLEAIRKGEPVPGSFAFDEIAREQLNWTYPHYEVTQIRTKQSVSEIKRKREYEDEYSGQAPVKPA
EGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTIEEAEQTVQKLYEKELLTEEQKAAIDIEEIVHFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFRHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=681937 M0696_RS05915 WP_248602592.1 1172974..1176678(+) (addA) [Bacillus rugosus strain A78.1]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTTTCAACCGGCCAGGATATTCT
CGTGGCAGCGGCGGCGGGCTCGGGTAAAACCGCTGTTCTCGTTGAACGAATGATACGGAAAATCACCGCAGAAGAAAACC
CGATTGATGTAGACCGCCTTCTCGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATAGCAGATGCC
TTGGAAAAAGAGCTTGTAAAACGCCCTGGCTCGCTGCATATCAGACGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTCCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTTGGGGATGAGGTGTTGGACGAGCTGTTTGAAGACGAATACGCAAAGGGAGAAAAGGCA
TTTTTTGAGCTTGTTGACCGCTATACGACAGACCGGCACGATCTGGACCTGCAATTTCTCGTCAAACAAGTGTACGAGTA
TTCCCGTTCAAACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTATATGATGTATCGGAAAAGAGTGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGCAATGGTGCTTAACGGAGCGAAGGAGAATCTGCAGCGCGCG
CTGGAGCTGACAAAAGCGCCGGGCGGCCCCGCCCCGCGCGCTGACACCTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGTATCAGGACGATTTCAATGAACTATATAGGCGTGTGCCTGCAGTCTCTTTTAAGCGCGCGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTGCGCTCCTTGAAGAAGCGACGGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
TCCGATTACTTCACGAGAAGTCCTGAACAGCATTTGAAAAGCTTGGCCGATATGAAGCCTGTCATTGAAACGCTCGTACA
GCTTGTTATCAGCTACGGAAAACGGTTCGAAGCCGCGAAACAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAATGACAAAGGTGAACGTGAGCCGAGCGAGGCGGCAAGATTTTATCAGGAACAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAATTTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGGCC
GGAGGAGACCGGTAACCTGTTTATGGTAGGAGATGTCAAACAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTTCTTT
TCCTCTCCAAATACAAACGGTTTACAGAGAGCGGAGAAGGCACGGGTCGGAAAATCGACTTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTCTATTTAAACAGCTGATGGGCGGCAAAATCGGTGAGGTCGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGATAATGATGAGACGGAAACGGAGCTGCTGCTGATCGACAACACAG
AAGAGACGGATGCAGGCGAGGAAGCAGAAGAGCTGGAAACGGTGCAGTTCGAGGCGAAAGCGATCGCCAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTACGCTCCATGCCGTGGGCGCCGCAAATCATGGAGGAGCTGAGAGCACAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGTTATTTTGAAGCGGTCGAAGTCGCCGTTGCGCTTTCCGTGCTGAAAGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTGCGCTCACCGATTGTCGGGGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATACAAA
AGCGCCGTACTATGAAGCGATGAAAGACTACTTGGCTGCTGGCGACCGGAACGATGAGCTTTATCAGAAGCTGCAAACAT
TCTACGGCCATCTGCAAAAATGGCGCGCGTTTTCGAAAAACCACTCTGTATCTGAGCTGATTTGGGAAGTGTACCGAGAC
ACGAAATATATGGATTATGTCGGCGGGATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGTGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGACTGTTCCGTTTCCTGCGGTTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGAGAGCGCTCAGCGAGCAGGAGGATGTTGTCCGCTTAATGACGATCCACAGCAGCAAGGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTCAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGATTTGGTTCGAAGTATATTCATCCGCAATTGCGCATCAGTTACCCGACGCTTCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGTTCGTGCAAGGATCATCAGAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAAACTGACTGGCTTCTTCCGGA
ATTTGACCGCTATCAGGCGAAAACCTATTTAGATTTCATTGGGCCGGCTCTTGCCAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGGTGCAGCAGCGCATGCTGATATATCAGGACATCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTGCTGGATGATGATTTGGAAGAAAGAATGGAAGAAAAAAGCGAGCGCTTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
TGGCTCGTTTGCATTTGATGAAATAGCCCGCGAGCAGCTGAACTGGACATATCCGCATTACGAGGTCACACAAATTCGTA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCAGGCCCCCGTTAAACCTGCT
GAAGGAAGCATTCTGTACAGGCGGCCCGCTTTTATGATGAAAAAAGGCCTGACAGCAGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTGACACATGTACCGACGATAGAAGAAGCGGAGCAGACGGTTCAAAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAGCCGCTATTGATATAGAAGAAATCGTGCATTTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGTGCTAAATGGAAGGATCGGGAAGTGCCATTCAGTTTAGCGCTTCCGGCCAAGGAGATCTATCCAGATGC
ACAGGAGGCGGATGAACCGCTTTTAGTGCAGGGAATCATAGATTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAATTCCGGCACGGCTTTGAAGGAGCGGCCCCTATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTTTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.191

100

0.962