Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   M0696_RS05910 Genome accession   NZ_CP096590
Coordinates   1169487..1172987 (+) Length   1166 a.a.
NCBI ID   WP_222142562.1    Uniprot ID   -
Organism   Bacillus rugosus strain A78.1     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1164487..1177987
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0696_RS05890 (M0696_05890) - 1165376..1166581 (-) 1206 WP_166854693.1 MFS transporter -
  M0696_RS05895 (M0696_05895) - 1166695..1168422 (+) 1728 WP_222142560.1 ABC transporter substrate-binding protein -
  M0696_RS05900 (M0696_05900) - 1168467..1168772 (-) 306 Protein_1098 four-helix bundle copper-binding protein -
  M0696_RS05905 (M0696_05905) - 1168866..1169303 (-) 438 WP_222142561.1 ferritin family protein -
  M0696_RS05910 (M0696_05910) addB 1169487..1172987 (+) 3501 WP_222142562.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  M0696_RS05915 (M0696_05915) addA 1172974..1176678 (+) 3705 WP_248602592.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  M0696_RS05920 (M0696_05920) sbcD 1176750..1177925 (+) 1176 WP_166853692.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134630.59 Da        Isoelectric Point: 5.3808

>NTDB_id=681936 M0696_RS05910 WP_222142562.1 1169487..1172987(+) (addB) [Bacillus rugosus strain A78.1]
MGAEFLVGRSGSGKTKLIIDSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRKLIEEHKQEFKVYQKASDKSGFTAQVERMLAEFKRYCLEPEDICRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLSEDVKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELNGTERHMQTPELAHLEAQYEARPAIPYAEKQEAFTVMQATNR
RAELEGIAREIHTLVREKGYRYKDVAILARQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSIDVLKGNWRY
EAVFRCVKTELLFPLNEPKTKVREQVDQLENYCIAYGIKGDRWTKGDRFHYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEEDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMMEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSIVVKRLEELFPQHKERLLTSEPEQVSDEEQVMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSDPDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLNLKERQFFKLEAPDIGQLFHSSLKLISDRLREQKMDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFKLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEAVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFELLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCAFKSVCQFDESLEENEYRSLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=681936 M0696_RS05910 WP_222142562.1 1169487..1172987(+) (addB) [Bacillus rugosus strain A78.1]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCGACAGCATTCAGGATGAACTGCG
CCGGGCTCCATTCGGGAAACCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCTGATATGGGCGGAATGATACGTGCTCAAGTGTTCAGCTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTTACGAGCACAGGCGTACAGATGCTCCTTCGGAAGCTCATTGAGGAGCATAAACAGGAGTT
TAAAGTCTATCAAAAAGCAAGTGATAAAAGCGGGTTTACCGCACAGGTTGAACGGATGCTGGCAGAGTTTAAGCGTTATT
GTTTGGAACCGGAAGATATCTGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTGTTTTATCTGAA
AAGCTTCATGATTTATCGATTCTGTATCAGCAAATGGAAAAAAGCCTTGCAGATCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATTCCGCTGTCGGAGGATGTAAAAGGCGCGCATATCTATGTGGACGGCTTTTACCAGTTTA
CACCTCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCAGAGCATATCACGTTTTCGCTGACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGAAAAACCTATTACCGCCTGCATCAGAAGGCCAA
GGAACTGAATCTGGATATTACCTATAAAGAGCTAAACGGAACTGAGCGGCATATGCAGACGCCAGAACTGGCGCATCTAG
AGGCGCAGTATGAAGCGCGTCCGGCTATCCCGTACGCAGAAAAACAAGAGGCCTTTACTGTAATGCAGGCCACAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATCCACACTCTTGTCAGAGAGAAGGGCTATCGCTATAAGGATGTAGCGAT
TCTTGCGCGCCAGCCGGAAGATTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGATG
GAAAAGCTTCTATGCTGAACCATCCGTTAATCGAATTTATCAGGTCGAGCATTGATGTTCTGAAAGGGAATTGGCGTTAT
GAAGCAGTGTTTCGCTGTGTGAAAACCGAACTGCTATTCCCGCTTAACGAGCCGAAGACTAAGGTGAGAGAACAGGTCGA
TCAGCTTGAAAATTACTGTATCGCCTACGGTATCAAAGGCGATCGCTGGACAAAGGGCGACCGTTTCCATTACAGACGTT
TTGTGTCATTGGATGATGATTTTGCGCAAACCGATCAGGAAATCGAAATGGAAAACATGCTGAATGACACACGCGACTGG
ATTGTTCCGCCGCTTTTTCAGCTCCAGAAAAGAATGAAGAAAGCAAAGACGGTTCAGGAGAAGGCGGAGGCGCTCTACCG
CTATCTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGACAGCGTGCTGAGGAAGACGGCAGAATCATTGAAG
CACAGCAGCATCAGCAGGCGTGGGATGCGGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTTTAATTCCGCCTGCCCTAGACCA
AGTGTTTGTCGGCAATATGGATTTATCCAGAATGTATGGCACCTCGTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGAGTGTTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGGTTGAGCTTTCC
TCAGGCGGGCGGGAGCGTCTGCTTGATGAGCATTTCCTCATCTATATGGCGTTTTCCAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCCGATGCGGAAGGAAAAACGCTTTTGCCGTCGATTGTCGTTAAGCGGCTGGAAGAACTGTTTCCGC
AGCATAAGGAGCGTCTGTTGACAAGTGAACCTGAACAGGTCAGCGATGAGGAACAGGTTATGTATGTCGTCAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGATTATGGACTAGGGAATACGACATCAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGATCCGGACAGACTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTAAAGC
AGCTTGAACGCAGCGTATCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTATCGAGAATGGAAACCTTTAATGCG
TGCCCGTTCTCCCATTTTGCATCGCACGGGCTCAATCTGAAGGAACGGCAATTTTTCAAGCTCGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACCGGCTGCGCGAGCAAAAAATGGATTGGCGCGATTTAACGAAAG
AGCAGTGCGAGCTGTTTTCCTATGATGCAGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAGATTTTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGCGTGTCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTTGTGCCAATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTAAGCTGAAAAACG
GCTGCACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAAGCGGAAAGCTCAAAAGGCCTGCTCCTAAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGGCTGGCGCTTCAAATGCTGACGTACCTTGA
TTTATCGATTACTCATTCAGCAGACTGGCTTGGCATGAGAGCGACACCGGCCGGAGTGCTGTATTTCCATATTCATGACC
CAATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCCTG
CTTCTCGGCGATCAGGAAGCTGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGATCAAATATCATCAACGCGGGCTT
GAAAAAAGACGGCTCTCTCAGATCAGACTCAGCGGCAGTCGGCGAAAAGGAATTTGAATTGTTGACGAAGCATGTGCGCC
GAACCTTCCAAGAAGCCGGCGAACAAATCACCGACGGACGCGTATCGATTGAGCCGTATAAAATGAAGAACAAGACGCCA
TGCACATACTGTGCGTTCAAATCAGTATGTCAATTTGATGAATCATTAGAAGAGAACGAGTATCGCTCGCTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

97.513

100

0.975