Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   M0696_RS00580 Genome accession   NZ_CP096590
Coordinates   105707..107083 (+) Length   458 a.a.
NCBI ID   WP_166854505.1    Uniprot ID   -
Organism   Bacillus rugosus strain A78.1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 100707..112083
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0696_RS00560 (M0696_00560) ctsR 101061..101525 (+) 465 WP_166854508.1 transcriptional regulator CtsR -
  M0696_RS00565 (M0696_00565) mcsA 101539..102096 (+) 558 WP_014475591.1 protein-arginine kinase activator protein McsA -
  M0696_RS00570 (M0696_00570) - 102096..103187 (+) 1092 WP_166854506.1 protein arginine kinase -
  M0696_RS00575 (M0696_00575) clpC 103184..105616 (+) 2433 WP_003225731.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  M0696_RS00580 (M0696_00580) radA 105707..107083 (+) 1377 WP_166854505.1 DNA repair protein RadA Machinery gene
  M0696_RS00585 (M0696_00585) disA 107087..108169 (+) 1083 WP_003242042.1 DNA integrity scanning diadenylate cyclase DisA -
  M0696_RS00590 (M0696_00590) - 108284..109384 (+) 1101 WP_166854504.1 PIN/TRAM domain-containing protein -
  M0696_RS00595 (M0696_00595) ispD 109399..110097 (+) 699 WP_166854503.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  M0696_RS00600 (M0696_00600) ispF 110090..110566 (+) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49483.98 Da        Isoelectric Point: 7.9805

>NTDB_id=681921 M0696_RS00580 WP_166854505.1 105707..107083(+) (radA) [Bacillus rugosus strain A78.1]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSDSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMTPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=681921 M0696_RS00580 WP_166854505.1 105707..107083(+) (radA) [Bacillus rugosus strain A78.1]
ATGGCTAAAACAAAATCGAAATTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
CGGTGCTTGGAACACAATGGTGGAAGAAATGATAAAAAAAGCACCAGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTCC
AAACCGTACAGAAACCCTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGGGTTAAAACCCAGCTTGGCGAATTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCACTCGTTTTAATCGGCGGCGATCCCGGTATCGGAAAGTCAACGTT
ATTACTGCAGGTTTCCGCTCAATTATCAGACTCATCGAACAGTGTGCTGTACATTTCGGGAGAGGAATCGGTCAAACAAA
CGAAACTAAGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGACTCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCGGGCAGTGTGTCACAGGTCAGAGAATGTACGGCTGAGCTGATGAAGATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCATGTGACGAAAGAAGGGTCTATTGCAGGGCCGAGACTGCTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGGGAACGTCACCATACTTTCCGTATTTTGAGGGCTGTAAAAAACCGTTTTGGTTCTACAAATGAAATGGGCATTTT
CGAAATGCGCGAAGAGGGGCTCACTGAGGTTTTGAACCCTTCAGAAATTTTCTTGGAAGAACGTTCAGCGGGATCTGCAG
GCTCAAGCATAACTGCTTCTATGGAGGGCACAAGGCCGATTCTCGTTGAAATACAGGCGCTAATCTCGCCAACAAGCTTT
GGCAATCCGCGGCGTATGGCAACGGGAATAGACCATAACAGGGTTTCGCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
GCTGCTGTTGCAAAATCAGGATGCCTATTTGAAAGTGGCTGGTGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCAATCAGTATCGCGTCCAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGTTTTATAGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTATCAAGAATTGAACAGCGTGTGAAAGAAGCGGCTAAGCTTGGTTTTAAACGCATGATTATACC
CGCGGCAAATCTGGATGGGTGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAGGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.127

100

0.991
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624