Detailed information NTG0681475

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: M1F51_RS05600
• Genome accession: NZ_CP096526
• Coordinates: 1116225..1116722 (+)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain RIVM_M087195
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	1116913..1118085	1116225..1116722	flank	191

Gene organization within MGE regions

Location: 1116225..1118085

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1F51_RS05600 (M1F51_05580)	comGF	1116225..1116722 (+)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
M1F51_RS05605 (M1F51_05585)	-	1116913..1118085 (+)	1173	WP_000195429.1	IS256-like element IS256 family transposase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=681475 M1F51_RS05600 WP_001825340.1 1116225..1116722(+) (comGF) [Staphylococcus aureus strain RIVM_M087195]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=681475 M1F51_RS05600 WP_001825340.1 1116225..1116722(+) (comGF) [Staphylococcus aureus strain RIVM_M087195]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97