Detailed information NTG0680644

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: M0536_RS22020
• Genome accession: NZ_CP096238
• Coordinates: 4534373..4535173 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain DD02201
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4529373..4540173

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M0536_RS21985 (M0536_21955)	gspH	4529529..4530044 (+)	516	WP_004183045.1	type II secretion system minor pseudopilin GspH	-
M0536_RS21990 (M0536_21960)	gspI	4530041..4530406 (+)	366	WP_060184290.1	type II secretion system minor pseudopilin GspI	-
M0536_RS21995 (M0536_21965)	gspJ	4530403..4530999 (+)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
M0536_RS22000 (M0536_21970)	gspK	4530996..4531982 (+)	987	WP_077250502.1	type II secretion system minor pseudopilin GspK	-
M0536_RS22005 (M0536_21975)	gspL	4531969..4533162 (+)	1194	WP_012737412.1	type II secretion system protein GspL	-
M0536_RS22010 (M0536_21980)	-	4533155..4533640 (+)	486	WP_012737411.1	type II secretion system protein M	-
M0536_RS22015 (M0536_21985)	-	4533630..4534376 (+)	747	WP_012737410.1	type II secretion system protein N	-
M0536_RS22020 (M0536_21990)	pilD	4534373..4535173 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
M0536_RS22025 (M0536_21995)	hrpB	4535221..4537650 (-)	2430	WP_012737409.1	ATP-dependent helicase HrpB	-
M0536_RS22030 (M0536_22000)	thpR	4537723..4538259 (+)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
M0536_RS22035 (M0536_22005)	sfsA	4538259..4538975 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
M0536_RS22040 (M0536_22010)	dksA	4539138..4539593 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=680644 M0536_RS22020 WP_004145898.1 4534373..4535173(+) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain DD02201]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=680644 M0536_RS22020 WP_004145898.1 4534373..4535173(+) (pilD) [Klebsiella pneumoniae subsp. pneumoniae strain DD02201]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425