Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MX651_RS23880 Genome accession   NZ_CP095920
Coordinates   5156941..5157813 (+) Length   290 a.a.
NCBI ID   WP_003107301.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AR19640     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5154254..5164421 5156941..5157813 within 0


Gene organization within MGE regions


Location: 5154254..5164421
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MX651_RS23870 (MX651_23870) - 5155483..5155719 (+) 237 Protein_4728 type IV-A pilus assembly ATPase PilB -
  MX651_RS23875 (MX651_23875) pilC 5155723..5156940 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  MX651_RS23880 (MX651_23880) pilD 5156941..5157813 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  MX651_RS23885 (MX651_23885) coaE 5157810..5158421 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  MX651_RS23890 (MX651_23890) yacG 5158418..5158618 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  MX651_RS23895 (MX651_23895) - 5158655..5158864 (-) 210 WP_003094660.1 hypothetical protein -
  MX651_RS23900 (MX651_23900) - 5158970..5159659 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  MX651_RS23905 (MX651_23905) - 5159656..5160126 (-) 471 WP_003103866.1 hypothetical protein -
  MX651_RS23910 (MX651_23910) - 5160123..5160548 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  MX651_RS23915 (MX651_23915) - 5160681..5161310 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  MX651_RS23920 (MX651_23920) - 5161307..5161756 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  MX651_RS23925 (MX651_23925) - 5161782..5161952 (+) 171 WP_003094672.1 DUF3094 family protein -
  MX651_RS23930 (MX651_23930) - 5162016..5163323 (+) 1308 WP_003112837.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31873.90 Da        Isoelectric Point: 7.7068

>NTDB_id=679051 MX651_RS23880 WP_003107301.1 5156941..5157813(+) (pilD) [Pseudomonas aeruginosa strain AR19640]
MTLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=679051 MX651_RS23880 WP_003107301.1 5156941..5157813(+) (pilD) [Pseudomonas aeruginosa strain AR19640]
ATGACCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCTTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

48.921

95.862

0.469