Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   MU858_RS26235 Genome accession   NZ_CP095874
Coordinates   5000704..5002053 (-) Length   449 a.a.
NCBI ID   WP_166688235.1    Uniprot ID   -
Organism   Bacillus sp. PGP15     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4995704..5007053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MU858_RS26200 (MU858_26200) - 4995729..4996424 (-) 696 WP_128805260.1 hypothetical protein -
  MU858_RS26205 (MU858_26205) - 4996411..4997199 (-) 789 WP_247998666.1 hypothetical protein -
  MU858_RS26210 (MU858_26210) - 4997231..4997800 (-) 570 WP_166688238.1 hypothetical protein -
  MU858_RS26215 (MU858_26215) - 4997918..4998400 (-) 483 WP_166688237.1 hypothetical protein -
  MU858_RS26220 (MU858_26220) raiA 4998813..4999355 (-) 543 WP_048528141.1 ribosome-associated translation inhibitor RaiA -
  MU858_RS26225 (MU858_26225) cspC 4999676..4999873 (-) 198 WP_001990088.1 cold shock protein CspC -
  MU858_RS26230 (MU858_26230) - 5000000..5000704 (-) 705 WP_166688236.1 ComF family protein -
  MU858_RS26235 (MU858_26235) comFA 5000704..5002053 (-) 1350 WP_166688235.1 ATP-dependent helicase ComFA Machinery gene
  MU858_RS26240 (MU858_26240) - 5002179..5003619 (-) 1441 Protein_5124 NlpC/P60 family protein -
  MU858_RS26245 (MU858_26245) - 5003768..5004082 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  MU858_RS26250 (MU858_26250) - 5004255..5005097 (-) 843 WP_048528137.1 DegV family protein -
  MU858_RS26255 (MU858_26255) - 5005338..5005973 (+) 636 WP_048528136.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51232.89 Da        Isoelectric Point: 9.7771

>NTDB_id=679025 MU858_RS26235 WP_166688235.1 5000704..5002053(-) (comFA) [Bacillus sp. PGP15]
MLAGKQLLLEELSSDLRKELSDLKKKGEVACVQGITKKASKYICQRCGNIEQRLFASFLCKRCSKRCTYCRKCITMGRVS
ECTVLVRGIQERKEERELNQLQWKGVLSTGQELAAQGVIKAIKQKESFFIWAVCGAGKTEMLFYGIAEALQKGERVCIAT
PRTDVVLELAPRLQEVFPNINIAALYGGSTDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKHNFKKGNQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLQHKKIPRVLLQWLKIYVNKKHPIFL
FVPHVRYIEEISLLLKELDNRIDGVHAEDSMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVMYFHYGKTESMVRAKKHILSMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=679025 MU858_RS26235 WP_166688235.1 5000704..5002053(-) (comFA) [Bacillus sp. PGP15]
ATGCTTGCTGGAAAACAGTTGCTATTAGAAGAACTCTCTTCAGATTTACGGAAAGAATTAAGTGACTTGAAAAAGAAGGG
AGAAGTCGCATGTGTACAAGGCATAACAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGAT
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAAGATGCACGTATTGCCGGAAGTGCATCACGATGGGGAGAGTTAGT
GAATGTACTGTACTCGTTCGTGGGATTCAGGAAAGAAAGGAAGAAAGGGAGTTAAATCAGTTGCAGTGGAAAGGGGTTTT
GTCTACCGGTCAGGAGTTGGCGGCGCAAGGTGTTATAAAAGCTATTAAGCAGAAAGAATCTTTCTTTATTTGGGCTGTAT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATTGCAGAGGCACTTCAAAAAGGAGAAAGAGTTTGTATTGCAACG
CCAAGAACAGACGTTGTACTGGAACTAGCACCGAGATTACAAGAAGTGTTTCCTAATATAAATATAGCTGCTTTATACGG
AGGGAGTACAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTCATGATTGTAGATGAAATTGATGCCTTCCCGTATCACGCAGATCAAATGTTACAGTATGCGGTGCAACAAGCGATG
AAAGAGAAAGCGGCGCGTATTTATTTAACAGCAACTCCTGATGAAAAGTGGAAGCATAATTTCAAAAAGGGGAATCAAAA
AGGTATCATTGTTTCAGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGCTGGTGCGGAAATTGGAAGAAAA
GCCTTCAGCATAAAAAAATTCCTCGAGTGTTACTACAATGGTTAAAAATATACGTAAACAAAAAACATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAAGTCTGTTATTGAAAGAATTGGATAATAGAATCGATGGTGTACATGC
AGAAGATTCGATGAGGAAAGAAAAAGTAGCAGCGTTCAGAAAGGGAGAAATTCCGTTATTAGTTACGACAACAATTTTAG
AAAGGGGAGTAACTGTGAAGAATTTACAAGTGGCGGTGCTAGGGGCAGAAGAAGAAATTTTTTCAGAGAGTGCGCTCGTA
CAAATTGCAGGCCGGGCGGGTCGTAGTTTTGAAGAGCCATATGGCGAGGTTATGTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGCGCGAAAAAACACATTCTAAGTATGAACAAAAATGCGAAGGAACAAGGGTTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(308-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.679

98.441

0.499
  comFA/cflA Streptococcus mitis SK321

37.385

97.105

0.363
  comFA Latilactobacillus sakei subsp. sakei 23K

40

90.2

0.361