Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MYW48_RS21955 Genome accession   NZ_CP095767
Coordinates   4323564..4324607 (-) Length   347 a.a.
NCBI ID   WP_001013181.1    Uniprot ID   -
Organism   Bacillus cereus strain CDHWZ7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4318564..4329607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MYW48_RS21910 (MYW48_21910) - 4318997..4319197 (-) 201 WP_000106083.1 YqzE family protein -
  MYW48_RS21915 (MYW48_21915) - 4319236..4319733 (-) 498 WP_000836666.1 shikimate kinase -
  MYW48_RS21920 (MYW48_21920) - 4319849..4320499 (-) 651 WP_000183864.1 2OG-Fe(II) oxygenase -
  MYW48_RS21925 (MYW48_21925) comGG 4320674..4321045 (-) 372 WP_001214886.1 competence type IV pilus minor pilin ComGG -
  MYW48_RS21930 (MYW48_21930) comGF 4321042..4321512 (-) 471 WP_000923110.1 competence type IV pilus minor pilin ComGF -
  MYW48_RS21935 (MYW48_21935) - 4321482..4321784 (-) 303 WP_000829465.1 hypothetical protein -
  MYW48_RS21940 (MYW48_21940) comGD 4321777..4322232 (-) 456 WP_002082168.1 competence type IV pilus minor pilin ComGD -
  MYW48_RS21945 (MYW48_21945) comGC 4322229..4322528 (-) 300 WP_001178680.1 competence type IV pilus major pilin ComGC -
  MYW48_RS21950 (MYW48_21950) comGB 4322540..4323577 (-) 1038 WP_106082358.1 competence type IV pilus assembly protein ComGB -
  MYW48_RS21955 (MYW48_21955) comGA 4323564..4324607 (-) 1044 WP_001013181.1 competence type IV pilus ATPase ComGA Machinery gene
  MYW48_RS21960 (MYW48_21960) - 4324813..4325508 (+) 696 WP_000434110.1 helix-turn-helix domain-containing protein -
  MYW48_RS21965 (MYW48_21965) - 4325635..4325877 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  MYW48_RS21970 (MYW48_21970) - 4325985..4327379 (+) 1395 WP_001094344.1 L-cystine transporter -
  MYW48_RS21975 (MYW48_21975) - 4327459..4327851 (-) 393 WP_106082346.1 hypothetical protein -
  MYW48_RS21980 (MYW48_21980) - 4327939..4328154 (-) 216 WP_142338634.1 DUF3912 family protein -
  MYW48_RS21985 (MYW48_21985) - 4328408..4328719 (+) 312 WP_001093238.1 hypothetical protein -
  MYW48_RS21990 (MYW48_21990) - 4328754..4329242 (-) 489 WP_000764268.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39168.55 Da        Isoelectric Point: 8.7212

>NTDB_id=678308 MYW48_RS21955 WP_001013181.1 4323564..4324607(-) (comGA) [Bacillus cereus strain CDHWZ7]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEANGEC
VTYKHETLESSIRKGYALGFLEDDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=678308 MYW48_RS21955 WP_001013181.1 4323564..4324607(-) (comGA) [Bacillus cereus strain CDHWZ7]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAATGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTTAAATTTTTAGCATCTATGGATATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCGACTGGTTCTGGGAAGACAACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGTATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTTACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAATGTCCGTTTTGCAGAGGGAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTGCTATATGGGTATGAGTTAAAACAAGCGCTTAAAGAAGCGAATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGGTTTTTAGAAGATGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369