Detailed information NTG0678224

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MMY93_RS23435
• Genome accession: NZ_CP095761
• Coordinates: 4855519..4856316 (+)
• Length: 265 a.a.
• NCBI ID: WP_086530433.1
• Uniprot ID: -
• Organism: Klebsiella michiganensis strain K254
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4850519..4861316

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMY93_RS23400 (MMY93_23400)	gspH	4850660..4851172 (+)	513	WP_197663590.1	type II secretion system minor pseudopilin GspH	-
MMY93_RS23405 (MMY93_23405)	gspI	4851169..4851534 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
MMY93_RS23410 (MMY93_23410)	gspJ	4851531..4852127 (+)	597	WP_047724421.1	type II secretion system minor pseudopilin GspJ	-
MMY93_RS23415 (MMY93_23415)	gspK	4852124..4853104 (+)	981	WP_109212803.1	type II secretion system minor pseudopilin GspK	-
MMY93_RS23420 (MMY93_23420)	gspL	4853097..4854290 (+)	1194	WP_009654536.1	type II secretion system protein GspL	-
MMY93_RS23425 (MMY93_23425)	-	4854283..4854768 (+)	486	WP_009654623.1	type II secretion system protein M	-
MMY93_RS23430 (MMY93_23430)	-	4854758..4855516 (+)	759	WP_197663591.1	type II secretion system protein N	-
MMY93_RS23435 (MMY93_23435)	pilD	4855519..4856316 (+)	798	WP_086530433.1	A24 family peptidase	Machinery gene
MMY93_RS23440 (MMY93_23440)	hrpB	4856414..4858843 (-)	2430	WP_197663592.1	ATP-dependent helicase HrpB	-
MMY93_RS23445 (MMY93_23445)	thpR	4858916..4859452 (+)	537	WP_086530431.1	RNA 2',3'-cyclic phosphodiesterase	-
MMY93_RS23450 (MMY93_23450)	sfsA	4859452..4860168 (+)	717	WP_004098690.1	DNA/RNA nuclease SfsA	-
MMY93_RS23455 (MMY93_23455)	dksA	4860333..4860788 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29064.69 Da
• Isoelectric Point: 7.5684

>NTDB_id=678224 MMY93_RS23435 WP_086530433.1 4855519..4856316(+) (pilD) [Klebsiella michiganensis strain K254]
METIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLFL
KGRSRCCGEPISPRYPLMELATGALFMLVGYLIPPGIPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=678224 MMY93_RS23435 WP_086530433.1 4855519..4856316(+) (pilD) [Klebsiella michiganensis strain K254]
ATGGAAACTATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGTTTCCTGTTTCTT
AAGGGACGTTCGCGCTGCTGCGGGGAACCCATCTCGCCTCGTTATCCTCTGATGGAGCTGGCGACCGGCGCGCTGTTTAT
GCTGGTCGGATACCTGATACCTCCGGGCATACCGCTGCTGGGCGGGCTAATTTTCGTATCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGTTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCTGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCCCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	47.431	95.472	0.453
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	45.174	97.736	0.442
pilD	Acinetobacter baumannii D1279779	44.788	97.736	0.438