Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   L8T27_RS13255 Genome accession   NZ_CP095743
Coordinates   2611206..2612267 (-) Length   353 a.a.
NCBI ID   WP_233313169.1    Uniprot ID   -
Organism   Niallia sp. Man26     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2606206..2617267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8T27_RS13215 (L8T27_013220) - 2607224..2608021 (+) 798 WP_237941696.1 YqhG family protein -
  L8T27_RS13220 (L8T27_013225) - 2608069..2608248 (-) 180 WP_233313179.1 YqzE family protein -
  L8T27_RS13225 (L8T27_013230) comGG 2608308..2608688 (-) 381 WP_233313178.1 competence type IV pilus minor pilin ComGG -
  L8T27_RS13230 (L8T27_013235) comGF 2608685..2609167 (-) 483 WP_237942319.1 competence type IV pilus minor pilin ComGF -
  L8T27_RS13235 (L8T27_013240) - 2609121..2609441 (-) 321 WP_237941697.1 hypothetical protein -
  L8T27_RS13240 (L8T27_013245) comGD 2609425..2609868 (-) 444 WP_233313175.1 competence type IV pilus minor pilin ComGD -
  L8T27_RS13245 (L8T27_013250) comGC 2609865..2610167 (-) 303 WP_233313173.1 competence type IV pilus major pilin ComGC Machinery gene
  L8T27_RS13250 (L8T27_013255) comGB 2610179..2611231 (-) 1053 WP_248574446.1 competence type IV pilus assembly protein ComGB -
  L8T27_RS13255 (L8T27_013260) comGA 2611206..2612267 (-) 1062 WP_233313169.1 competence type IV pilus ATPase ComGA Machinery gene
  L8T27_RS28715 - 2612421..2612555 (+) 135 WP_282581400.1 hypothetical protein -
  L8T27_RS13260 (L8T27_013265) - 2612693..2612788 (+) 96 WP_233313168.1 YjcZ family sporulation protein -
  L8T27_RS13270 (L8T27_013275) - 2613146..2613865 (+) 720 WP_237941699.1 helix-turn-helix domain-containing protein -
  L8T27_RS13275 (L8T27_013280) - 2613900..2614535 (-) 636 WP_237942321.1 MBL fold metallo-hydrolase -
  L8T27_RS13280 (L8T27_013285) - 2614564..2614878 (-) 315 WP_233313165.1 MTH1187 family thiamine-binding protein -
  L8T27_RS13285 (L8T27_013290) - 2614959..2616074 (-) 1116 WP_237941700.1 hypothetical protein -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 39353.69 Da        Isoelectric Point: 9.6625

>NTDB_id=678043 L8T27_RS13255 WP_233313169.1 2611206..2612267(-) (comGA) [Niallia sp. Man26]
MISIEALANRIIREAVEANATDIHIVPKGKESHLQFRISHSLYPRMTLPVNQCDRLISHFKFTASMDIGERRRPQNGAIR
TSANGENISLRLSTLPASPHESLVIRLLPDKNSIPLTRISLFPNTTKKLLSLLKHAHGLVIFTGPTGSGKTTTLYSLLSS
SSYLYKRKIISLEDPVEKPQDNVLQVQVNEKAGITYSAGLKAILRHDPDVIMVGEIRDAETARIAVRASLTGHLVLATMH
TRDAKGAIYRLMEFGISKCEVQQTLIAVTAQRLVELVCPFCGPVCLPFCFQLQNRINRASVFEIMTGRTIQSILAETFAE
KLNPRPDLREAINKGVALGFVKETEYGRWIYED

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=678043 L8T27_RS13255 WP_233313169.1 2611206..2612267(-) (comGA) [Niallia sp. Man26]
TTGATTTCTATTGAAGCTTTAGCGAACCGTATTATTCGGGAAGCTGTAGAAGCGAATGCCACAGATATTCACATTGTTCC
GAAAGGAAAAGAATCACATTTGCAATTTCGTATTTCCCACTCTTTATACCCCAGAATGACACTCCCTGTGAATCAATGCG
ACAGACTTATTTCCCATTTTAAGTTTACCGCCTCTATGGATATTGGCGAAAGGCGGCGCCCGCAAAACGGTGCAATCCGT
ACAAGTGCAAACGGGGAAAACATCAGCCTCCGACTGTCCACCTTGCCTGCAAGTCCCCATGAAAGCCTCGTCATCCGTCT
GCTGCCAGACAAGAATAGTATACCGCTCACCCGTATATCTCTTTTCCCAAACACCACTAAAAAGCTTCTGTCACTGCTTA
AACATGCCCATGGGCTTGTTATTTTCACAGGGCCGACTGGGTCAGGCAAAACAACAACCCTTTATTCTCTTCTCAGCAGC
AGCTCCTATCTGTACAAACGAAAAATCATTAGTCTCGAGGACCCAGTTGAAAAGCCGCAGGATAACGTGCTTCAAGTGCA
GGTTAATGAAAAAGCAGGCATTACGTATTCAGCGGGGCTAAAGGCAATATTGCGCCATGATCCAGATGTAATTATGGTTG
GCGAAATAAGGGATGCAGAAACAGCTCGAATTGCTGTCAGAGCCTCGTTAACAGGGCATCTTGTGCTTGCGACTATGCAC
ACAAGAGATGCGAAAGGGGCAATCTATCGACTCATGGAATTCGGCATCAGCAAGTGTGAGGTGCAGCAAACTTTAATTGC
TGTTACAGCGCAAAGGCTTGTCGAGCTTGTTTGTCCTTTTTGCGGTCCTGTTTGTCTGCCCTTTTGCTTTCAGCTTCAAA
ACCGCATAAACAGAGCCAGTGTTTTTGAAATAATGACAGGGAGGACCATTCAGTCCATTCTCGCTGAAACCTTTGCTGAA
AAGCTTAATCCTCGTCCTGATTTAAGAGAGGCTATTAATAAAGGAGTTGCCCTGGGGTTTGTAAAGGAGACAGAGTATGG
TCGCTGGATTTATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

54.648

100

0.55
  pilB Glaesserella parasuis strain SC1401

38.372

97.45

0.374
  pilB Haemophilus influenzae 86-028NP

37.209

97.45

0.363