Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SAMI_RS08250 Genome accession   NZ_AP017320
Coordinates   1655280..1655777 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain MI     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1650280..1660777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAMI_RS08225 (SAMI_1536) gcvPB 1650442..1651914 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SAMI_RS08230 (SAMI_1537) gcvPA 1651907..1653253 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAMI_RS08235 (SAMI_1538) gcvT 1653273..1654364 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  SAMI_RS08240 (SAMI_1539) - 1654523..1655047 (-) 525 WP_001015120.1 shikimate kinase -
  SAMI_RS08245 - 1655037..1655183 (-) 147 WP_001792109.1 hypothetical protein -
  SAMI_RS08250 (SAMI_1540) comGF 1655280..1655777 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAMI_RS08255 (SAMI_1541) comGE 1655695..1655994 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  SAMI_RS08260 (SAMI_1542) comGD 1655981..1656427 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAMI_RS08265 (SAMI_1543) comGC 1656405..1656716 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SAMI_RS08270 (SAMI_1544) comGB 1656730..1657800 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAMI_RS08275 (SAMI_1545) comGA 1657772..1658746 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SAMI_RS08280 (SAMI_1546) - 1658798..1659421 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SAMI_RS08285 (SAMI_1547) - 1659418..1659747 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAMI_RS08290 (SAMI_1548) - 1659747..1660733 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=67725 SAMI_RS08250 WP_001788897.1 1655280..1655777(-) (comGF) [Staphylococcus aureus strain MI]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=67725 SAMI_RS08250 WP_001788897.1 1655280..1655777(-) (comGF) [Staphylococcus aureus strain MI]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment