Detailed information NTG0677188

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: MW696_RS21230
• Genome accession: NZ_CP095476
• Coordinates: 4171001..4174693 (+)
• Length: 1230 a.a.
• NCBI ID: WP_247071005.1
• Uniprot ID: -
• Organism: Bacillus glycinifermentans strain MGMM1
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4166001..4179693

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MW696_RS21220 (MW696_21225)	modB	4166749..4167405 (+)	657	WP_048355538.1	molybdate ABC transporter permease subunit	-
MW696_RS21225 (MW696_21230)	addB	4167514..4171014 (+)	3501	WP_247071003.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MW696_RS21230 (MW696_21235)	addA	4171001..4174693 (+)	3693	WP_247071005.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MW696_RS21235 (MW696_21240)	sbcD	4174761..4175945 (+)	1185	WP_048356282.1	exonuclease subunit SbcD	-
MW696_RS21240 (MW696_21245)	sbcC	4175942..4179334 (+)	3393	WP_247071007.1	exonuclease subunit SbcC	-
MW696_RS21245 (MW696_21250)	-	4179394..4179612 (-)	219	WP_046131288.1	spore germination protein	-

Sequence

Protein

• Length: 1230 a.a.
• Molecular weight: 141275.12 Da
• Isoelectric Point: 5.7739

>NTDB_id=677188 MW696_RS21230 WP_247071005.1 4171001..4174693(+) (addA) [Bacillus glycinifermentans strain MGMM1]
METAKPKNSTWTDDQWKAIVSSGRDILVAAAAGSGKTAVLVERIIRKITAEENPIDVDRLLVVTFTNASAAEMKHRIGEA
LEKKLAENPGSLHLRRQLSLLNRASISTLHSFCLQVVRKYYYMIDVDPAFRIADQTEGELLGDEVLDELFEEEYQKGDPA
FFELVDRYTTDRHDLDLQYLIKRVYEFSRSHPDPEAWLEKFMDLYDADPGAKIESLSFYSYIREDITLVLNSVREKLTRA
LELTKRPGGPAPRAENFLDDLAQIDKLLEHQDDFAALYELVPAVSFQRAKPCKGDDFDPSLIDEATDLRNSAKKQLEKLK
GDYFTRTPEQHLASLKEMKPVIRTLVQLVAEFGRRYRAAKKEKAIVDFSDLEHDCLAILTKKNEGGTAEPSEAAAYYRHL
FHEVLVDEYQDTNLVQEAILKLVAKEEHEGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTDDGSGAGQKIDLNKNFRSR
SDILDSTNFLFKQLMGEKVGEVAYDEQAELKLGASYPPNESTKTEVLIIENREDAADEEAEELETVQLEARAVAGRIREL
ISGKFQVYDGKTKTPRNIQYRDIVILLRSMPWAPQFMDEFKQQGIPVYANLSTGYFEATEVAVTLSLLKVIDNAYQDIPL
ASVLRSPIVGLDENELSLIRIRDKKAPFYEALKAYMASADKDDPLYEKLARFDGFLKKWRAYAKNHSVAELIWEVYRDTK
YIDYVGGMPGGKQRQANLRALYDRARSYESTSFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLEFP
VVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPKWRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFLVG
TAKDKDKLLAKWRTSASHAEWLLPDFERFQAKSYLDFIGPALMRHRDMADGDLNLPNDDIRQHQSRFTISWISAGELQAE
GRAQTEEEKHERLSLVKKGLPIDGSFDYEKEVRERLTWSYPFRDASQVRTKQSVSEIKRQKEYEDEYGDRSLVRPANETL
LFRRPSFMMAKGLTAAERGTAMHTVMQHLPLTRVPEKDELGRMLDRLIEKELLTEEQRAAIEEDDILAFFDTDIGGKMLS
ARRVEREVPFNMTLPAREVYPGLKTADEPVLIQGIIDCLFETDDGFYLLDYKTDRIHGKFQNGFEGAEPILRKRYATQIN
LYARAVETMLKTPLRGKALYFFDGGHVIMF

Nucleotide

• Length: 3693 bp

>NTDB_id=677188 MW696_RS21230 WP_247071005.1 4171001..4174693(+) (addA) [Bacillus glycinifermentans strain MGMM1]
ATGGAAACGGCTAAGCCGAAAAACAGCACATGGACGGACGACCAATGGAAAGCGATCGTCTCATCCGGCCGGGATATTTT
GGTCGCCGCTGCGGCGGGCTCCGGAAAAACGGCCGTCCTTGTCGAGCGGATCATCCGAAAGATTACGGCGGAGGAAAATC
CGATCGATGTCGACCGGCTGCTCGTCGTCACCTTTACGAATGCATCGGCGGCGGAGATGAAGCACCGGATCGGTGAAGCG
CTTGAGAAAAAGCTCGCCGAAAACCCCGGCTCCCTTCATTTGCGCAGGCAGCTTTCCTTGCTGAACAGGGCGAGTATTTC
GACATTGCACTCTTTTTGCCTTCAGGTCGTCCGCAAGTATTATTATATGATCGACGTTGATCCGGCCTTTCGGATCGCCG
ATCAGACAGAAGGCGAGCTGCTCGGTGATGAGGTGCTTGACGAGCTGTTTGAAGAAGAATATCAAAAGGGAGATCCGGCT
TTTTTTGAGCTGGTGGACAGATATACGACAGACCGCCACGATCTCGATTTGCAGTACCTTATCAAGCGGGTTTACGAGTT
TTCCCGCTCACACCCCGACCCGGAAGCGTGGCTTGAAAAATTTATGGACCTGTATGACGCAGATCCCGGCGCGAAGATCG
AGTCGCTGTCGTTTTATTCCTACATCAGGGAAGATATCACGCTTGTATTGAACAGTGTGCGTGAAAAGCTGACCCGTGCG
CTTGAGCTGACAAAACGGCCGGGGGGGCCGGCTCCGCGCGCTGAAAACTTTCTCGACGATTTGGCGCAGATCGACAAGCT
GCTTGAGCATCAGGACGATTTTGCGGCCCTTTATGAGCTTGTGCCGGCTGTTTCGTTCCAGCGGGCCAAACCGTGCAAAG
GGGATGATTTCGATCCGTCTTTAATCGACGAAGCGACAGACCTTCGCAACAGTGCAAAAAAACAGCTTGAAAAGCTGAAA
GGCGACTATTTCACGCGAACGCCAGAGCAGCATTTGGCGAGTTTGAAGGAGATGAAGCCTGTCATCAGGACGCTTGTCCA
GCTCGTTGCAGAGTTCGGCAGACGCTATAGGGCCGCGAAGAAAGAAAAAGCGATCGTAGACTTCTCCGATCTTGAGCACG
ACTGCCTGGCGATTTTGACAAAGAAAAACGAAGGCGGAACAGCGGAGCCGAGCGAAGCTGCGGCCTATTACCGGCACTTG
TTCCACGAAGTGCTCGTTGATGAATATCAGGATACAAACCTTGTACAGGAGGCGATTTTAAAGCTTGTCGCCAAGGAGGA
ACACGAAGGGAACCTCTTTATGGTGGGCGATGTCAAGCAGTCGATCTACCGTTTCCGGCTGGCTGAGCCGCTTTTGTTTC
TTTCGAAATACAAGCGGTTCACAGATGACGGCTCAGGCGCCGGACAGAAAATCGATTTGAACAAAAACTTCAGAAGCCGG
TCCGACATTTTGGACAGCACCAATTTTTTGTTTAAACAGCTGATGGGAGAGAAGGTCGGCGAGGTTGCCTATGACGAACA
AGCCGAATTAAAGCTCGGCGCATCGTATCCTCCCAATGAAAGCACAAAAACAGAGGTGCTGATCATTGAAAACCGGGAAG
ACGCGGCTGATGAAGAGGCGGAAGAGCTTGAAACGGTTCAGCTTGAGGCAAGGGCTGTCGCCGGACGGATCAGGGAGCTG
ATTTCCGGCAAATTTCAAGTGTATGACGGTAAAACGAAAACGCCAAGAAACATCCAGTACCGCGACATCGTGATTTTGCT
GCGCTCCATGCCGTGGGCGCCGCAATTCATGGACGAGTTCAAGCAGCAGGGGATTCCGGTATACGCGAACCTGTCAACCG
GATATTTCGAAGCGACAGAAGTAGCCGTCACGCTTTCACTGCTTAAAGTCATCGACAATGCCTATCAGGATATTCCGCTT
GCATCCGTCCTCAGGTCACCGATTGTCGGCCTTGATGAAAACGAACTGTCCCTGATCAGAATCCGGGATAAAAAAGCGCC
GTTCTATGAAGCGCTGAAGGCGTATATGGCGTCAGCGGACAAAGATGACCCGCTTTATGAAAAGCTTGCCCGCTTTGACG
GGTTCCTGAAAAAGTGGAGGGCATACGCAAAAAATCATTCCGTGGCGGAGCTGATATGGGAAGTTTACCGCGATACGAAA
TATATCGATTATGTCGGCGGAATGCCGGGCGGAAAGCAGCGGCAGGCCAATTTGCGCGCGCTCTATGACAGGGCGAGATC
GTACGAATCGACCTCCTTCCGCGGATTGTTCCGCTTTTTGCGATTTATTGAACGGATGCAGGAGCGGGGCGATGACCTCG
GAACGGCTCGGGCTTTGAGCGAACAGGAAGACGTCGTCCGCCTGATGACGATCCATTCGAGCAAAGGTCTTGAATTTCCC
GTCGTATTCACGGCAGGCCTCGGCCGGAATTTCAATATGATGGATCTCAACAAATCGTATCTGCTTGATAAAGAGCTCGG
GTTCGGGACGAAATTTATCCATCCGAAATGGCGCATCAGCTATCCGACGCTGCCGCTTGTTGCGATGAAGAAAAAAATGC
GGCGCGAGCTTTTATCAGAAGAGCTTCGCGTCTTGTATGTCGCTCTCACCCGTGCAAAGGAAAAACTCTTTTTGGTCGGA
ACGGCGAAAGACAAGGACAAGCTTTTGGCAAAATGGCGGACCTCCGCCTCCCATGCCGAATGGCTGCTCCCTGATTTTGA
ACGCTTTCAGGCGAAATCGTATCTCGATTTTATCGGACCCGCCTTAATGCGGCACAGGGATATGGCAGACGGCGATCTGA
ACTTGCCGAATGATGACATCCGTCAGCATCAGTCCCGTTTTACGATCAGCTGGATATCAGCCGGAGAGCTGCAGGCCGAA
GGTAGAGCGCAGACTGAAGAAGAAAAACACGAACGGCTGTCACTGGTGAAAAAAGGGCTGCCGATCGATGGCTCTTTCGA
TTATGAAAAAGAGGTCCGCGAGCGGCTGACATGGTCGTATCCTTTCCGGGATGCGTCACAAGTCCGCACAAAACAGTCTG
TCTCTGAAATCAAGAGGCAGAAGGAATACGAGGATGAATACGGCGACCGCTCATTGGTTCGTCCGGCAAATGAAACTTTG
CTGTTCAGAAGACCCTCTTTCATGATGGCAAAAGGGCTGACCGCCGCCGAACGGGGAACGGCGATGCATACAGTCATGCA
GCATCTTCCGCTCACCCGCGTCCCCGAAAAGGATGAGCTCGGCCGTATGCTGGACAGGCTTATCGAAAAAGAACTTCTAA
CGGAAGAGCAACGCGCGGCTATCGAAGAAGACGACATTCTCGCGTTTTTCGATACGGATATAGGCGGAAAAATGCTCAGC
GCCCGCCGGGTTGAGCGGGAAGTGCCCTTCAACATGACGCTTCCTGCACGAGAGGTTTATCCGGGCTTGAAAACTGCCGA
TGAACCCGTCTTAATTCAAGGGATTATCGACTGCTTGTTCGAAACCGATGACGGCTTTTATTTGCTCGACTACAAAACCG
ACCGGATACACGGCAAATTTCAAAACGGCTTTGAAGGAGCGGAGCCGATTTTGCGAAAACGCTACGCCACACAGATCAAC
CTTTACGCCCGGGCTGTTGAAACGATGCTTAAGACGCCTCTGAGAGGTAAGGCTCTTTACTTTTTCGACGGCGGGCACGT
GATCATGTTTTGA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (471-880)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-460)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1051-1222)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	73.496	100	0.735