Detailed information NTG0067644

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FIS3754_RS15340
• Genome accession: NZ_AP017305
• Coordinates: 3734115..3735221 (+)
• Length: 368 a.a.
• NCBI ID: WP_062247845.1
• Uniprot ID: A0A2N6LYH8
• Organism: Fischerella sp. NIES-3754
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3729115..3740221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIS3754_RS15315 (FIS3754_32360)	-	3729122..3729634 (-)	513	WP_035137979.1	TIGR02652 family protein	-
FIS3754_RS25600	-	3729665..3729847 (-)	183	WP_016871528.1	hypothetical protein	-
FIS3754_RS15320 (FIS3754_32370)	-	3729837..3730364 (+)	528	WP_062247840.1	gamma carbonic anhydrase family protein	-
FIS3754_RS15325 (FIS3754_32380)	-	3730818..3730943 (+)	126	WP_009457343.1	photosystem II protein Y	-
FIS3754_RS15330 (FIS3754_32390)	-	3731084..3732406 (+)	1323	WP_062247842.1	folylpolyglutamate synthase/dihydrofolate synthase family protein	-
FIS3754_RS15335 (FIS3754_32400)	-	3732684..3733985 (-)	1302	WP_062247843.1	sugar ABC transporter substrate-binding protein	-
FIS3754_RS15340 (FIS3754_32410)	pilM	3734115..3735221 (+)	1107	WP_062247845.1	type IV pilus biogenesis protein PilM	Machinery gene
FIS3754_RS15345 (FIS3754_32420)	-	3735538..3736302 (+)	765	WP_062247847.1	PilN domain-containing protein	-
FIS3754_RS15350 (FIS3754_32430)	-	3736299..3737054 (+)	756	WP_062247848.1	hypothetical protein	-
FIS3754_RS15355 (FIS3754_32440)	-	3737489..3739930 (+)	2442	WP_062247849.1	type IV pilus secretin family protein	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 40474.51 Da
• Isoelectric Point: 4.4224

>NTDB_id=67644 FIS3754_RS15340 WP_062247845.1 3734115..3735221(+) (pilM) [Fischerella sp. NIES-3754]
MVKSINNLFSKSRGGIGIELAPQCVKVARLRKQRQGLKLDALTSVPVPEGVFVDGQIADPPAMAQIIQQALTESKIKASR
VATAVTGRDSIVRLIPVPSELDDQELREMVLNHEASLYLPYPREEADVDYQKLGYFIDEDGIEKVQVLLVATRKEITDTY
INTFEQAGLQIDVLEINSFALIRTIRDQLRQYGPQEAAVLVDIEFDSTEIAIIVNGVPQFSRTVPLGTYQMQMALARAMS
LPASRDMELLQGMSIPLNTMEGAKTGVTEANPGVAAMMRVLGELTDELRRSIDFYLNQTENLEVAQIFLSGPGGGLAQLD
EFFTQRLSLPTSQIDPIGSLSLQVDEEKFPSVQRPGLGIVLGLGMREV

Nucleotide

• Length: 1107 bp

>NTDB_id=67644 FIS3754_RS15340 WP_062247845.1 3734115..3735221(+) (pilM) [Fischerella sp. NIES-3754]
GTGGTTAAAAGCATCAATAATCTGTTTAGCAAATCTAGAGGTGGAATTGGCATCGAACTGGCGCCACAATGCGTCAAGGT
CGCTCGTTTACGCAAACAACGCCAAGGTCTGAAACTAGACGCTTTAACATCAGTACCGGTTCCAGAAGGTGTTTTTGTTG
ATGGTCAAATTGCTGATCCTCCAGCAATGGCACAAATCATTCAGCAGGCACTCACAGAGAGTAAAATAAAAGCTTCTCGG
GTTGCCACTGCTGTCACAGGACGAGATTCAATTGTGCGCCTGATACCCGTTCCATCAGAATTAGATGATCAAGAGCTACG
CGAGATGGTGTTAAACCATGAAGCAAGCTTATATTTACCCTATCCTCGTGAAGAAGCTGATGTAGATTATCAGAAACTTG
GGTACTTTATAGATGAAGATGGGATTGAAAAAGTACAAGTGCTACTGGTTGCAACTCGCAAGGAAATTACTGATACCTAC
ATAAATACATTCGAGCAGGCAGGGTTACAAATTGATGTCTTAGAAATCAATTCTTTTGCACTGATTCGGACAATTCGTGA
CCAGTTGCGGCAATACGGGCCACAGGAAGCAGCAGTTTTAGTCGATATAGAATTCGACAGCACAGAAATTGCCATTATTG
TCAACGGAGTACCACAATTTTCACGGACAGTCCCCTTGGGAACTTATCAAATGCAAATGGCACTGGCCAGAGCAATGAGT
TTACCTGCATCACGAGATATGGAATTATTGCAGGGGATGAGTATTCCTCTCAATACTATGGAAGGCGCAAAAACGGGTGT
CACCGAAGCTAATCCTGGTGTCGCAGCAATGATGCGGGTGTTGGGAGAACTGACAGATGAACTACGCCGTTCCATCGATT
TTTATCTCAACCAAACTGAAAATTTAGAGGTAGCACAAATTTTCTTGTCAGGTCCGGGTGGAGGATTGGCACAGTTAGAT
GAGTTCTTCACACAAAGATTGAGTTTACCGACCTCCCAAATAGACCCGATAGGATCTTTGTCTTTGCAAGTTGACGAAGA
AAAGTTTCCCAGTGTGCAACGTCCTGGGTTGGGGATAGTGCTGGGTCTTGGGATGCGGGAGGTGTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (283-367)

• Type IV pilus assembly protein PilM; (PilM_2) (17-238)

3D structure

• AlphaFold DB: A0A2N6LYH8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Synechocystis sp. PCC 6803	63.76	99.728	0.636