Detailed information NTG0676107

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MU300_RS19605
• Genome accession: NZ_CP095240
• Coordinates: 3997745..3998545 (-)
• Length: 266 a.a.
• NCBI ID: WP_002888947.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain 21080237
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3992745..4003545

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU300_RS19585 (MU300_19580)	dksA	3993325..3993780 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
MU300_RS19590 (MU300_19585)	sfsA	3993943..3994659 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MU300_RS19595 (MU300_19590)	thpR	3994659..3995195 (-)	537	WP_004145901.1	RNA 2',3'-cyclic phosphodiesterase	-
MU300_RS19600 (MU300_19595)	hrpB	3995268..3997697 (+)	2430	WP_004151944.1	ATP-dependent helicase HrpB	-
MU300_RS19605 (MU300_19600)	pilD	3997745..3998545 (-)	801	WP_002888947.1	A24 family peptidase	Machinery gene
MU300_RS19610 (MU300_19605)	-	3998542..3999288 (-)	747	WP_004145897.1	type II secretion system protein N	-
MU300_RS19615 (MU300_19610)	-	3999278..3999763 (-)	486	WP_004151943.1	type II secretion system protein M	-
MU300_RS19620 (MU300_19615)	gspL	3999756..4000949 (-)	1194	WP_004151942.1	type II secretion system protein GspL	-
MU300_RS19625 (MU300_19620)	gspK	4000936..4001922 (-)	987	WP_004151941.1	type II secretion system minor pseudopilin GspK	-
MU300_RS19630 (MU300_19625)	gspJ	4001919..4002515 (-)	597	WP_004151940.1	type II secretion system minor pseudopilin GspJ	-
MU300_RS19635 (MU300_19630)	gspI	4002512..4002877 (-)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MU300_RS19640 (MU300_19635)	gspH	4002874..4003389 (-)	516	WP_004151939.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29419.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=676107 MU300_RS19605 WP_002888947.1 3997745..3998545(-) (pilD) [Klebsiella pneumoniae strain 21080237]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGSIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=676107 MU300_RS19605 WP_002888947.1 3997745..3998545(-) (pilD) [Klebsiella pneumoniae strain 21080237]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTAGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGA
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGAGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425