Detailed information NTG0675753

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MU411_RS21730
• Genome accession: NZ_CP095150
• Coordinates: 4437024..4437824 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Iso00073
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4432024..4442824

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU411_RS21695	gspH	4432177..4432692 (+)	516	WP_021313445.1	type II secretion system minor pseudopilin GspH	-
MU411_RS21700	gspI	4432689..4433054 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MU411_RS21705	gspJ	4433051..4433647 (+)	597	WP_012737414.1	type II secretion system minor pseudopilin GspJ	-
MU411_RS21710	gspK	4433644..4434630 (+)	987	WP_004178610.1	type II secretion system minor pseudopilin GspK	-
MU411_RS21715	gspL	4434617..4435813 (+)	1197	WP_064145608.1	type II secretion system protein GspL	-
MU411_RS21720	-	4435806..4436291 (+)	486	WP_023312675.1	type II secretion system protein M	-
MU411_RS21725	-	4436281..4437027 (+)	747	WP_004145897.1	type II secretion system protein N	-
MU411_RS21730	pilD	4437024..4437824 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
MU411_RS21735	hrpB	4437872..4440301 (-)	2430	WP_064145607.1	ATP-dependent helicase HrpB	-
MU411_RS21740	thpR	4440374..4440910 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
MU411_RS21745	sfsA	4440910..4441626 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MU411_RS21750	dksA	4441789..4442244 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=675753 MU411_RS21730 WP_004145898.1 4437024..4437824(+) (pilD) [Klebsiella pneumoniae strain Iso00073]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=675753 MU411_RS21730 WP_004145898.1 4437024..4437824(+) (pilD) [Klebsiella pneumoniae strain Iso00073]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425