Detailed information NTG0675729

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MU412_RS21170
• Genome accession: NZ_CP095145
• Coordinates: 4312663..4313463 (+)
• Length: 266 a.a.
• NCBI ID: WP_004191960.1
• Uniprot ID: A0AAJ5B1F9
• Organism: Klebsiella pneumoniae strain Iso00100
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4307663..4318463

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU412_RS21135	gspH	4307819..4308334 (+)	516	WP_004213437.1	type II secretion system minor pseudopilin GspH	-
MU412_RS21140	gspI	4308331..4308696 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MU412_RS21145	gspJ	4308693..4309289 (+)	597	WP_004145893.1	type II secretion system minor pseudopilin GspJ	-
MU412_RS21150	gspK	4309286..4310272 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
MU412_RS21155	gspL	4310259..4311452 (+)	1194	WP_032416685.1	type II secretion system protein GspL	-
MU412_RS21160	-	4311445..4311930 (+)	486	WP_004145896.1	type II secretion system protein M	-
MU412_RS21165	-	4311920..4312666 (+)	747	WP_004145897.1	type II secretion system protein N	-
MU412_RS21170	pilD	4312663..4313463 (+)	801	WP_004191960.1	A24 family peptidase	Machinery gene
MU412_RS21175	hrpB	4313511..4315940 (-)	2430	WP_032416684.1	ATP-dependent helicase HrpB	-
MU412_RS21180	thpR	4316013..4316549 (+)	537	WP_032416682.1	RNA 2',3'-cyclic phosphodiesterase	-
MU412_RS21185	sfsA	4316549..4317265 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MU412_RS21190	dksA	4317428..4317883 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29403.04 Da
• Isoelectric Point: 8.8894

>NTDB_id=675729 MU412_RS21170 WP_004191960.1 4312663..4313463(+) (pilD) [Klebsiella pneumoniae strain Iso00100]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMI

Nucleotide

• Length: 801 bp

>NTDB_id=675729 MU412_RS21170 WP_004191960.1 4312663..4313463(+) (pilD) [Klebsiella pneumoniae strain Iso00100]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTGCTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGATCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGATCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.057	99.624	0.489
pilD	Vibrio campbellii strain DS40M4	46.545	100	0.481
pilD	Acinetobacter nosocomialis M2	46.388	98.872	0.459
pilD	Acinetobacter baumannii D1279779	45.627	98.872	0.451
pilD	Neisseria gonorrhoeae MS11	43.922	95.865	0.421