Detailed information NTG0675705

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MU413_RS22115
• Genome accession: NZ_CP095140
• Coordinates: 4433402..4434202 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain Iso00199
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4428402..4439202

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU413_RS22080	gspH	4428558..4429073 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
MU413_RS22085	gspI	4429070..4429435 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MU413_RS22090	gspJ	4429432..4430028 (+)	597	WP_023302298.1	type II secretion system minor pseudopilin GspJ	-
MU413_RS22095	gspK	4430025..4431011 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
MU413_RS22100	gspL	4430998..4432191 (+)	1194	WP_029498035.1	type II secretion system protein GspL	-
MU413_RS22105	-	4432184..4432669 (+)	486	WP_004145896.1	type II secretion system protein M	-
MU413_RS22110	-	4432659..4433405 (+)	747	WP_004145897.1	type II secretion system protein N	-
MU413_RS22115	pilD	4433402..4434202 (+)	801	WP_004145898.1	A24 family peptidase	Machinery gene
MU413_RS22120	hrpB	4434250..4436679 (-)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
MU413_RS22125	thpR	4436752..4437288 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
MU413_RS22130	sfsA	4437288..4438004 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MU413_RS22135	dksA	4438167..4438622 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=675705 MU413_RS22115 WP_004145898.1 4433402..4434202(+) (pilD) [Klebsiella pneumoniae strain Iso00199]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=675705 MU413_RS22115 WP_004145898.1 4433402..4434202(+) (pilD) [Klebsiella pneumoniae strain Iso00199]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425