NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	MU415_RS21950	Genome accession	NZ_CP095132
Coordinates	4476412..4477212 (+)	Length	266 a.a.
NCBI ID	WP_004213441.1	Uniprot ID	A0A9Q4U0T4
Organism	Klebsiella pneumoniae strain Iso00267
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4471412..4482212

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MU415_RS21915	gspH	4471568..4472083 (+)	516	WP_020804560.1	type II secretion system minor pseudopilin GspH	-
MU415_RS21920	gspI	4472080..4472445 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
MU415_RS21925	gspJ	4472442..4473038 (+)	597	WP_032411156.1	type II secretion system minor pseudopilin GspJ	-
MU415_RS21930	gspK	4473035..4474021 (+)	987	WP_004183041.1	type II secretion system minor pseudopilin GspK	-
MU415_RS21935	gspL	4474008..4475201 (+)	1194	WP_020804558.1	type II secretion system protein GspL	-
MU415_RS21940	-	4475194..4475679 (+)	486	WP_004145896.1	type II secretion system protein M	-
MU415_RS21945	-	4475669..4476415 (+)	747	WP_094617437.1	type II secretion system protein N	-
MU415_RS21950	pilD	4476412..4477212 (+)	801	WP_004213441.1	A24 family peptidase	Machinery gene
MU415_RS21955	hrpB	4477260..4479689 (-)	2430	WP_020804570.1	ATP-dependent helicase HrpB	-
MU415_RS21960	thpR	4479762..4480298 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
MU415_RS21965	sfsA	4480298..4481014 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
MU415_RS21970	dksA	4481177..4481632 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

Download Length: 266 a.a. Molecular weight: 29405.01 Da Isoelectric Point: 8.8894

                                >NTDB_id=675658 MU415_RS21950 WP_004213441.1 4476412..4477212(+) (pilD) [Klebsiella pneumoniae strain Iso00267]

MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPISWRDNIPLLSFLWL

GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN

EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ

SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

Download Length: 801 bp

                                >NTDB_id=675658 MU415_RS21950 WP_004213441.1 4476412..4477212(+) (pilD) [Klebsiella pneumoniae strain Iso00267]

ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG

CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA

CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCTCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG

GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT

CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC

GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC

GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT

CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC

AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTACTGCAACGCCTGTGGACCCGGCAG

TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA

G

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-109)

Type IV leader peptidase family (Peptidase_A24)

(123-231)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425

Detailed information NTG0675658