Detailed information NTG0674769

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MT472_RS24295
• Genome accession: NZ_CP094999
• Coordinates: 5049216..5050013 (+)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain fxq
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5044216..5055013

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MT472_RS24260 (MT472_24260)	gspH	5044357..5044869 (+)	513	WP_042944313.1	type II secretion system minor pseudopilin GspH	-
MT472_RS24265 (MT472_24265)	gspI	5044866..5045231 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
MT472_RS24270 (MT472_24270)	gspJ	5045228..5045824 (+)	597	WP_014837428.1	type II secretion system minor pseudopilin GspJ	-
MT472_RS24275 (MT472_24275)	gspK	5045821..5046801 (+)	981	WP_042944311.1	type II secretion system minor pseudopilin GspK	-
MT472_RS24280 (MT472_24280)	gspL	5046794..5047987 (+)	1194	WP_042944310.1	type II secretion system protein GspL	-
MT472_RS24285 (MT472_24285)	-	5047980..5048465 (+)	486	WP_042944309.1	type II secretion system protein M	-
MT472_RS24290 (MT472_24290)	-	5048455..5049213 (+)	759	WP_042944307.1	type II secretion system protein N	-
MT472_RS24295 (MT472_24295)	pilD	5049216..5050013 (+)	798	WP_014227970.1	A24 family peptidase	Machinery gene
MT472_RS24300 (MT472_24300)	hrpB	5050111..5052540 (-)	2430	WP_014227969.1	ATP-dependent helicase HrpB	-
MT472_RS24305 (MT472_24305)	thpR	5052613..5053149 (+)	537	WP_014227968.1	RNA 2',3'-cyclic phosphodiesterase	-
MT472_RS24310 (MT472_24310)	sfsA	5053149..5053865 (+)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
MT472_RS24315 (MT472_24315)	dksA	5054030..5054485 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=674769 MT472_RS24295 WP_014227970.1 5049216..5050013(+) (pilD) [Klebsiella michiganensis strain fxq]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=674769 MT472_RS24295 WP_014227970.1 5049216..5050013(+) (pilD) [Klebsiella michiganensis strain fxq]
ATGGAAAATATCGCGCTGTTACCGGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGTCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434