Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MUA25_RS08135 Genome accession   NZ_CP094801
Coordinates   1654497..1655471 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain IVB6155     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1649497..1660471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MUA25_RS08095 (MUA25_08090) gcvT 1649998..1651089 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  MUA25_RS08100 (MUA25_08095) - 1651248..1651772 (-) 525 WP_001015125.1 shikimate kinase -
  MUA25_RS08105 (MUA25_08100) - 1651762..1651908 (-) 147 WP_001789879.1 hypothetical protein -
  MUA25_RS08110 (MUA25_08105) comGF 1652005..1652502 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  MUA25_RS08115 (MUA25_08110) comGE 1652420..1652719 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  MUA25_RS08120 (MUA25_08115) comGD 1652706..1653152 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  MUA25_RS08125 (MUA25_08120) comGC 1653130..1653441 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  MUA25_RS08130 (MUA25_08125) comGB 1653455..1654525 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  MUA25_RS08135 (MUA25_08130) comGA 1654497..1655471 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  MUA25_RS08140 (MUA25_08135) - 1655524..1656147 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  MUA25_RS08145 (MUA25_08140) - 1656144..1656473 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  MUA25_RS08150 (MUA25_08145) - 1656473..1657459 (-) 987 WP_000161314.1 ROK family glucokinase -
  MUA25_RS08155 (MUA25_08150) - 1657456..1657659 (-) 204 WP_000087561.1 YqgQ family protein -
  MUA25_RS08160 (MUA25_08155) - 1657640..1659103 (-) 1464 WP_045179195.1 rhomboid family intramembrane serine protease -
  MUA25_RS08165 (MUA25_08160) - 1659115..1659654 (-) 540 WP_000164553.1 5-formyltetrahydrofolate cyclo-ligase -
  MUA25_RS08170 (MUA25_08165) rpmG 1659863..1660012 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=672882 MUA25_RS08135 WP_000697220.1 1654497..1655471(-) (comGA) [Staphylococcus aureus strain IVB6155]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=672882 MUA25_RS08135 WP_000697220.1 1654497..1655471(-) (comGA) [Staphylococcus aureus strain IVB6155]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTGCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1