Detailed information NTG0671485

Bioinformatically predicted

Overview

• Name: ccrA
• Type: Machinery gene
• Locus tag: MUA70_RS00280
• Genome accession: NZ_CP094731
• Coordinates: 60180..61541 (-)
• Length: 453 a.a.
• NCBI ID: WP_108703124.1
• Uniprot ID: -
• Organism: Staphylococcus epidermidis strain IVB6208
• Function: promote SCCmec transfer (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
SCCmec	36469..60183	60180..61541	flank	-3

Gene organization within MGE regions

Location: 36469..61541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MUA70_RS00170 (MUA70_00170)	ccrA	37795..39156 (-)	1362	WP_061544228.1	cassette chromosome recombinase CcrA	Machinery gene
MUA70_RS00175 (MUA70_00175)	cch1	39367..41133 (-)	1767	WP_061544227.1	cassette chromosome replicative helicase	-
MUA70_RS00180 (MUA70_00180)	ssb	41133..41426 (-)	294	WP_046464617.1	cassette chromosome ssDNA-binding protein	-
MUA70_RS00185 (MUA70_00185)	-	41597..43111 (-)	1515	WP_108703122.1	hypothetical protein	-
MUA70_RS00190 (MUA70_00190)	-	43742..44653 (+)	912	WP_046464619.1	ABC transporter substrate-binding protein	-
MUA70_RS00195 (MUA70_00195)	-	44903..45564 (-)	662	Protein_36	IS6 family transposase	-
MUA70_RS00200 (MUA70_00200)	-	45678..46634 (+)	957	WP_000282655.1	thymidylate synthase	-
MUA70_RS00205 (MUA70_00205)	-	46676..47161 (+)	486	WP_000175735.1	trimethoprim-resistant dihydrofolate reductase DfrS1	-
MUA70_RS00210 (MUA70_00210)	-	47171..47569 (+)	399	Protein_39	DegV family protein	-
MUA70_RS00215 (MUA70_00215)	-	47625..48299 (-)	675	WP_001106019.1	IS6-like element IS257 family transposase	-
MUA70_RS00220 (MUA70_00220)	-	48335..48724 (+)	390	WP_020444595.1	hydroxymethylglutaryl-CoA synthase	-
MUA70_RS00225 (MUA70_00225)	-	49601..50344 (+)	744	WP_000958858.1	glycerophosphoryl diester phosphodiesterase	-
MUA70_RS00230 (MUA70_00230)	-	50441..50869 (+)	429	WP_000872606.1	MaoC family dehydratase	-
MUA70_RS00235 (MUA70_00235)	mecA	50915..52924 (-)	2010	WP_001801873.1	PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA	-
MUA70_RS00240 (MUA70_00240)	-	53021..54007 (+)	987	WP_001549960.1	beta-lactam sensor/signal transducer MecR1	-
MUA70_RS00245 (MUA70_00245)	-	53994..54239 (+)	246	Protein_46	type I restriction enzyme endonuclease domain-containing protein	-
MUA70_RS00250 (MUA70_00250)	-	54230..55840 (-)	1611	Protein_47	IS1182 family transposase	-
MUA70_RS00255 (MUA70_00255)	-	55889..56395 (-)	507	WP_001092195.1	DUF1643 domain-containing protein	-
MUA70_RS00260 (MUA70_00260)	-	56410..56721 (-)	312	WP_001024803.1	DUF960 family protein	-
MUA70_RS00265 (MUA70_00265)	-	56808..57158 (-)	351	WP_070508371.1	SAUGI family uracil-DNA glycosylase inhibitor	-
MUA70_RS00270 (MUA70_00270)	-	57841..58431 (-)	591	WP_070508372.1	hypothetical protein	-
MUA70_RS00275 (MUA70_00275)	ccrB	58558..60183 (-)	1626	WP_262622810.1	cassette chromosome recombinase CcrB	Machinery gene
MUA70_RS00280 (MUA70_00280)	ccrA	60180..61541 (-)	1362	WP_108703124.1	cassette chromosome recombinase CcrA	Machinery gene

Sequence

Protein

• Length: 453 a.a.
• Molecular weight: 53216.10 Da
• Isoelectric Point: 9.8409

>NTDB_id=671485 MUA70_RS00280 WP_108703124.1 60180..61541(-) (ccrA) [Staphylococcus epidermidis strain IVB6208]
MEEAIAYVRQSTLKQQSLATQKSLIMDTAKQYGWSNVTFYDDKKTGRHTKRSGYQKMVEAIRSGKCKVLCCYRLNRLHRN
LKNAIQFFEICKTHHITIISVNDGYFNLAKEFDCFRLNILMSLAEMESNNISEQTKNGIREKAKQGKLITTHAPFGYRYR
QSHFIVHEEEAYTVKAVYRWYLQGIGYKKISQHLDNHPKLIPRKPYQVRNILLNPNYCGRVINKYGTFNDIVPSIIDVDT
FEEAQERRIHKQHNRSNSRNKLKRRIKCPYCQSTLTNLTIKKEKHSLRYYVCPKNMNEAYHTCPFKGINAQMIETEVLNT
CKAYCDNQSFHDKLNETILKALKHQQMKHRQSHLTQEQLIEKLAQNQIDIETFKHLSAGSESEERYSNYSHKQITKTIRH
IIKDKLTLETIAPFIDCINITQTKQLQGIYFKNSPLNIVEQSHLIISERNEVV

Nucleotide

• Length: 1362 bp

>NTDB_id=671485 MUA70_RS00280 WP_108703124.1 60180..61541(-) (ccrA) [Staphylococcus epidermidis strain IVB6208]
ATGGAAGAAGCGATTGCTTATGTACGTCAAAGTACACTCAAACAACAAAGCTTAGCTACTCAAAAATCGCTCATTATGGA
TACCGCTAAGCAATATGGATGGTCTAATGTGACGTTTTATGATGATAAGAAAACAGGTCGACATACAAAACGTTCGGGCT
ATCAAAAAATGGTAGAAGCGATTCGTTCTGGAAAATGTAAAGTATTGTGTTGCTATCGCCTAAATCGGCTCCATCGTAAT
TTGAAAAATGCCATACAATTCTTTGAAATTTGTAAAACACATCATATCACGATTATCAGTGTTAACGATGGCTATTTTAA
CTTAGCTAAAGAGTTTGATTGTTTTCGCTTGAACATACTTATGAGTCTAGCGGAAATGGAATCAAACAATATCAGCGAAC
AAACAAAAAATGGGATTAGAGAAAAAGCCAAACAAGGTAAGCTAATCACAACTCATGCTCCGTTTGGCTATCGTTATAGA
CAAAGTCATTTTATTGTACATGAAGAAGAAGCGTATACAGTTAAAGCGGTCTATCGCTGGTACTTACAGGGTATAGGCTA
CAAAAAGATTTCCCAACATTTAGATAACCATCCTAAGCTTATTCCTCGTAAGCCCTATCAAGTTCGAAATATTCTCTTGA
ACCCTAACTATTGTGGTCGTGTCATTAATAAATATGGAACGTTTAATGATATCGTTCCATCTATTATTGATGTTGATACG
TTTGAAGAAGCACAAGAGCGTCGTATTCACAAACAACACAATCGATCCAATTCTAGAAATAAGCTAAAGCGACGAATCAA
ATGTCCTTACTGTCAATCAACATTAACAAACTTAACGATAAAAAAAGAGAAACACTCTCTACGTTACTACGTTTGTCCTA
AAAATATGAATGAAGCCTATCATACGTGTCCATTTAAAGGTATCAATGCGCAAATGATTGAAACAGAGGTCCTCAACACT
TGTAAAGCATATTGTGACAATCAGTCATTTCATGACAAATTAAATGAGACAATACTAAAAGCTCTAAAACACCAACAAAT
GAAACACAGACAATCACATCTAACACAAGAACAACTCATCGAAAAACTGGCACAAAATCAAATTGATATCGAGACGTTTA
AACATTTATCTGCTGGCTCTGAAAGTGAAGAACGTTACTCTAATTATTCACATAAACAAATTACCAAAACAATCCGTCAT
ATAATCAAAGATAAATTGACATTAGAAACCATAGCACCTTTTATCGATTGCATCAACATAACTCAAACTAAACAATTACA
AGGAATCTACTTTAAAAATAGTCCACTCAATATCGTTGAACAATCACACTTAATAATATCTGAAAGGAATGAAGTCGTAT
GA

Domains

Predicted by InterproScan.

• Resolvase, N terminal domain (Resolvase) (4-147)

• Recombinase (Recombinase) (168-248)

• Recombinase zinc beta ribbon domain (Zn_ribbon_recom) (262-321)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ccrA	Staphylococcus aureus COL	55.305	97.792	0.541
ccrA	Staphylococcus aureus N315	52.144	97.792	0.51