Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KF707C_RS04505 Genome accession   NZ_AP014862
Coordinates   963828..965534 (-) Length   568 a.a.
NCBI ID   WP_003452478.1    Uniprot ID   A0AAD1BWY5
Organism   Metapseudomonas furukawaii strain KF707     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 958828..970534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KF707C_RS04475 (KF707C_8810) - 958838..959305 (+) 468 WP_004420098.1 hypothetical protein -
  KF707C_RS04480 (KF707C_8820) - 959302..959988 (+) 687 WP_004420096.1 energy-coupling factor ABC transporter permease -
  KF707C_RS29895 - 960107..960232 (-) 126 WP_269148406.1 hypothetical protein -
  KF707C_RS04485 (KF707C_8840) yacG 960928..961128 (-) 201 WP_003452484.1 DNA gyrase inhibitor YacG -
  KF707C_RS04490 (KF707C_8850) coaE 961125..961736 (-) 612 WP_003452481.1 dephospho-CoA kinase -
  KF707C_RS04495 (KF707C_8860) pilD 961733..962605 (-) 873 WP_003452480.1 A24 family peptidase Machinery gene
  KF707C_RS04500 (KF707C_8870) pilC 962608..963825 (-) 1218 WP_003452479.1 type II secretion system F family protein Machinery gene
  KF707C_RS04505 (KF707C_8880) pilB 963828..965534 (-) 1707 WP_003452478.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KF707C_RS04510 (KF707C_8890) - 965808..966257 (+) 450 WP_003452477.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KF707C_RS04515 (KF707C_8900) - 966330..968324 (+) 1995 WP_003452476.1 sulfatase-like hydrolase/transferase -
  KF707C_RS04520 (KF707C_8910) - 968362..969996 (+) 1635 WP_158526749.1 O-antigen ligase -
  KF707C_RS04530 (KF707C_8920) - 970151..970378 (-) 228 WP_003452474.1 hypothetical protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62813.88 Da        Isoelectric Point: 5.8901

>NTDB_id=67141 KF707C_RS04505 WP_003452478.1 963828..965534(-) (pilB) [Metapseudomonas furukawaii strain KF707]
MNETAPALSGLAKQLVLTHLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAAEQFGVAFLDLSSIDKESQPR
ELVSEKLIRQHRALPLWRRGNKLFVAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDGAGSGMEELADVDL
DGLDVEAVDDNKQDSPGAVDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERSYRVRFRTDGMLHEVAKPPVQLATRIAA
RLKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNTLPTLWGEKIVMRILDATSAQMGIDALGYEDEQKNLYMEALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCVHCRKEHEVPRETLIKEGF
PEARIGSFKLYSPVGCDNCNQGYKGRVGIYEVVKITPALQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=67141 KF707C_RS04505 WP_003452478.1 963828..965534(-) (pilB) [Metapseudomonas furukawaii strain KF707]
ATGAACGAAACCGCTCCCGCGCTCTCCGGCCTGGCCAAACAACTGGTGCTGACCCATCTGCTGGATGAGCGAAGCGCGCT
GCAGGCCCATACCCAGGCCCAGCGGAACAAGATTCCGCTGATCACCTACCTGGTGCAGAACAAGCTGCTAAAAAGCCGCG
ACCTGGCGGAAATGGCGGCGGAGCAGTTCGGCGTCGCCTTTCTCGACCTGTCCAGCATCGACAAGGAGAGCCAGCCCAGG
GAGTTGGTGAGCGAAAAGCTGATTCGCCAGCACCGCGCCCTGCCCCTGTGGCGTCGGGGCAACAAGCTGTTCGTCGCCAT
CTCCGACCCCACCAACCACCAGGCCGTCACCGATATCCAGTTCAGCACCGGGCTCAATACCGAAGCCATCCTGGTGGAGG
ACGACAAGCTGGGGGACGCCATCGAGAAGTTCTTCGACGGCGCCGGCAGCGGCATGGAAGAGCTGGCGGATGTGGACCTG
GACGGCCTCGACGTCGAGGCGGTCGACGATAACAAGCAGGACAGCCCGGGCGCCGTGGATGCCGACGATGCGCCAGTGGT
GCGCTTCGTCAACAAGATGCTGCTGGATGCCATTCGCGGCGGTTCGTCTGACCTGCACTTCGAACCCTACGAGCGCAGCT
ACCGCGTGCGCTTCCGTACCGACGGCATGCTCCACGAGGTGGCCAAGCCGCCGGTGCAGTTGGCCACCCGCATCGCCGCC
CGCCTGAAGGTGATGGCCGCACTGGATATTTCCGAGCGCCGCAAGCCCCAGGACGGCCGGATCAAGATGAAGATTTCCAA
GACGAAATCCATCGACTTCCGGGTGAACACCCTGCCCACCCTCTGGGGCGAAAAGATCGTGATGCGGATCCTCGACGCCA
CCAGCGCCCAGATGGGCATCGATGCCCTGGGCTACGAGGATGAGCAGAAGAACCTCTATATGGAGGCGCTGAAGCAGCCC
CAGGGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTCTCCCTCTACACTGGCCTGAATATCCTCAACAC
CGTGGACATCAATATCTCCACGGCGGAAGACCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAACCCCA
AGCAGGGCCTGGACTTCGCCCAGGCGCTGAGGGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGCGAGATCCGC
GACCTGGAGACGGCGGAAATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGTTGTCCACCCTGCACACCAACAGCGC
CGCGGAAACCCTGACCCGCCTGCGCAACATGGGGGTGCCGGCCTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCC
AGCGCCTGGCACGCAAGTTGTGCGTCCATTGCAGGAAGGAGCATGAGGTGCCCCGGGAGACCCTGATCAAGGAGGGCTTC
CCGGAGGCGCGCATCGGCAGTTTCAAACTCTATAGCCCGGTGGGCTGCGACAACTGCAACCAGGGTTACAAGGGCCGAGT
GGGTATTTATGAAGTGGTTAAAATCACGCCAGCCCTGCAGCAGCTTATTATGGAAGAAGGCAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGCGATGGCTTCAATGACCTGCGAACCTCCGGCCTGGTGAAAGCCATGAACGGCATCACCAGCCTGGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-148)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

58.127

99.648

0.579
  pilB Acinetobacter baylyi ADP1

57.848

99.824

0.577
  pilB Acinetobacter baumannii D1279779

57.746

100

0.577
  pilB Vibrio parahaemolyticus RIMD 2210633

50.707

99.648

0.505
  pilB Vibrio campbellii strain DS40M4

49.472

100

0.495
  pilB Vibrio cholerae strain A1552

53.042

92.606

0.491
  pilF Neisseria gonorrhoeae MS11

49.201

99.12

0.488
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

93.134

0.368

Multiple sequence alignment