Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MTX16_RS08310 Genome accession   NZ_CP094624
Coordinates   1631171..1632214 (-) Length   347 a.a.
NCBI ID   WP_001013169.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain LX43     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1626171..1637214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MTX16_RS08265 - 1626604..1626804 (-) 201 WP_000106083.1 YqzE family protein -
  MTX16_RS08270 - 1626843..1627340 (-) 498 WP_017673552.1 shikimate kinase -
  MTX16_RS08275 - 1627456..1628106 (-) 651 WP_000183863.1 2OG-Fe(II) oxygenase -
  MTX16_RS08280 comGG 1628281..1628652 (-) 372 WP_001231316.1 competence type IV pilus minor pilin ComGG -
  MTX16_RS08285 comGF 1628649..1629119 (-) 471 WP_000923101.1 competence type IV pilus minor pilin ComGF -
  MTX16_RS08290 - 1629089..1629391 (-) 303 WP_061457704.1 hypothetical protein -
  MTX16_RS08295 comGD 1629384..1629839 (-) 456 WP_000813141.1 competence type IV pilus minor pilin ComGD -
  MTX16_RS08300 comGC 1629836..1630135 (-) 300 WP_001178687.1 competence type IV pilus major pilin ComGC -
  MTX16_RS08305 comGB 1630147..1631184 (-) 1038 WP_061457705.1 competence type IV pilus assembly protein ComGB -
  MTX16_RS08310 comGA 1631171..1632214 (-) 1044 WP_001013169.1 competence type IV pilus ATPase ComGA Machinery gene
  MTX16_RS08315 - 1632420..1633115 (+) 696 WP_000434125.1 helix-turn-helix domain-containing protein -
  MTX16_RS08320 - 1633241..1633483 (+) 243 WP_000440716.1 DUF2626 domain-containing protein -
  MTX16_RS08325 - 1633591..1634985 (+) 1395 WP_001094344.1 L-cystine transporter -
  MTX16_RS08330 - 1635065..1635457 (-) 393 Protein_1671 hypothetical protein -
  MTX16_RS08335 - 1635545..1635760 (-) 216 WP_001008326.1 DUF3912 family protein -
  MTX16_RS08340 - 1636013..1636324 (+) 312 WP_001093237.1 hypothetical protein -
  MTX16_RS08345 - 1636359..1636847 (-) 489 WP_000764267.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39153.53 Da        Isoelectric Point: 8.5415

>NTDB_id=670261 MTX16_RS08310 WP_001013169.1 1631171..1632214(-) (comGA) [Bacillus thuringiensis strain LX43]
MNGIEIFANAILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGYELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=670261 MTX16_RS08310 WP_001013169.1 1631171..1632214(-) (comGA) [Bacillus thuringiensis strain LX43]
ATGAATGGGATTGAAATTTTTGCGAATGCAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCGGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGCGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTTGTGTTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATTATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGTGATGAAGAAACAGCAAAAATAGCTATAAGAGCAAGTTTGACAGGACATTTAGTAATGACAACACTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGCTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGAC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGATATGAGTTAAAACAAGCGCTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559
  pilB Haemophilus influenzae 86-028NP

39.752

92.795

0.369