Detailed information NTG0066941

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: GM3709_RS04410
• Genome accession: NZ_AP014821
• Coordinates: 1022178..1024907 (+)
• Length: 909 a.a.
• NCBI ID: WP_066121727.1
• Uniprot ID: A0A0D6AP34
• Organism: Geminocystis sp. NIES-3709
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1017178..1029907

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GM3709_RS04390 (GM3709_915)	-	1018189..1018944 (-)	756	WP_066116648.1	DUF2993 domain-containing protein	-
GM3709_RS04395 (GM3709_916)	hpnH	1019520..1020533 (+)	1014	WP_066116649.1	adenosyl-hopene transferase HpnH	-
GM3709_RS04400 (GM3709_917)	pilH	1021011..1021376 (+)	366	WP_066121722.1	response regulator transcription factor	Machinery gene
GM3709_RS04405 (GM3709_918)	-	1021582..1022121 (+)	540	WP_173645699.1	chemotaxis protein CheW	-
GM3709_RS04410 (GM3709_919)	pilJ	1022178..1024907 (+)	2730	WP_066121727.1	methyl-accepting chemotaxis protein	Machinery gene
GM3709_RS04415 (GM3709_920)	-	1025073..1025837 (+)	765	WP_066116651.1	MlaE family lipid ABC transporter permease subunit	-
GM3709_RS04420 (GM3709_921)	-	1026009..1026944 (+)	936	WP_066116652.1	NAD-dependent epimerase/dehydratase family protein	-
GM3709_RS04425 (GM3709_922)	-	1027404..1029074 (+)	1671	WP_066116654.1	NAD+ synthase	-

Sequence

Protein

• Length: 909 a.a.
• Molecular weight: 100494.13 Da
• Isoelectric Point: 3.9346

>NTDB_id=66941 GM3709_RS04410 WP_066121727.1 1022178..1024907(+) (pilJ) [Geminocystis sp. NIES-3709]
MVSETDYQQRYGQAQTAYLEGDLESAINIADDITKDYPEDPSVLLLKGHIYLRQQEYETAKATYKQVLNLTDHQDLVNLA
HQGLAQIEEEQQDFYPSEVESPMFDDEIEEIDDDEPNDNWTSSIQFDSIDWDVDDLEEEDIEDPTLQQNPPLNRDFSNPP
PKPKSSTQQNESFENLFTDDDNDDTAANQTPSKNEKVTSHSDFDGFSDDLLAMNLDESDFNYDDEDEDFISDGTQDTGAP
TFVVSSEEPELSDLEKMLTGGANLLGDFDEDLGDYSSELEPLTSAMQSSNPSTTELGDEDGPLVPEGEENFFFAPDELDD
IPDIDADELPVSSIFTKEQRQDKSFSSILDDNDNVFDDDFENEITGSFTFDTDKINLDIEDDDISVSSSLSDISTSPSST
IFTPEVEIPQGNLAKYYNLPLLSKQLVHGGITGIATFLIVLLVSFSQASIPLNTTNKPEVKPTTEKKETPVKQEDKQAKT
ITYPFFNRFLLSLIAGLASGGSTIALGYLMTNHVRRYTNDLQNQFESVYQGDYEVKATVYSQDEFGTLAAGFNQMAKMIN
TTTTEARKRAEETERAREDLQRQVIRLLDDVEGAARGDLTVQAEVTADVLGAVADAFNLTITNLRKIVKQVKQAAVQVNK
ASTDSEVFARNQSSDALRMAEELAVTLNSVQMMTDSIQRVAENAREAEEVARSSSVTALKGGDAVERTVAGILQIRETVS
ETTRKVKRLAEASQQISTIVAVISQISSRTNLLALNASIQAARAGEAGRGFAIVADEVRQLADRSAKSLQEIEQIVLQIQ
TETGSVMTAMEEGIQQVRDVTDRSEQAKRSLEDIIQVSNRIDALVRSITADTDEQRENSRGVAKVMQAVELTAQATSQES
QRVAGSLQNLVGIAKDLISSVERFKVEGK

Nucleotide

• Length: 2730 bp

>NTDB_id=66941 GM3709_RS04410 WP_066121727.1 1022178..1024907(+) (pilJ) [Geminocystis sp. NIES-3709]
ATGGTATCAGAAACTGACTATCAACAAAGATATGGACAGGCTCAAACAGCTTATCTTGAGGGTGATTTAGAATCAGCTAT
TAATATTGCAGATGACATTACAAAAGACTACCCAGAAGATCCGAGCGTTTTATTACTCAAAGGACATATATACTTACGTC
AACAAGAATACGAAACAGCGAAAGCTACCTATAAACAAGTACTGAATCTAACAGATCATCAAGATTTAGTAAACTTAGCT
CATCAAGGATTAGCACAAATAGAAGAAGAACAACAAGACTTTTACCCCTCAGAAGTAGAGTCCCCTATGTTTGACGATGA
GATTGAAGAAATAGATGATGATGAACCAAATGATAATTGGACGAGTAGTATTCAATTTGATAGCATCGATTGGGATGTGG
ATGATTTAGAGGAAGAAGACATCGAAGATCCCACTCTGCAACAAAATCCCCCTCTTAATAGAGATTTCTCTAATCCTCCT
CCAAAACCAAAATCTTCTACTCAACAAAATGAGTCTTTTGAAAATCTATTTACCGATGATGATAATGATGATACCGCAGC
GAATCAAACCCCCAGTAAAAATGAAAAAGTTACCTCACATTCAGATTTTGACGGATTTTCTGATGATCTGTTAGCCATGA
ATCTTGATGAATCAGATTTTAACTATGATGATGAGGATGAAGATTTTATCAGTGATGGTACTCAAGATACAGGTGCCCCT
ACTTTTGTTGTTTCTTCTGAAGAACCAGAATTGAGTGATCTCGAAAAAATGTTAACGGGAGGAGCCAATCTTTTAGGAGA
TTTTGACGAAGATTTAGGAGACTATAGCTCAGAATTAGAACCTTTAACTTCTGCAATGCAATCATCTAACCCCTCAACTA
CTGAATTAGGAGATGAAGATGGCCCCTTAGTACCAGAAGGAGAGGAAAATTTCTTTTTTGCACCTGATGAATTAGACGAT
ATTCCTGATATTGATGCAGATGAATTACCTGTATCTAGTATTTTCACTAAAGAACAAAGACAAGACAAATCCTTTAGTAG
TATTTTAGATGATAATGATAATGTTTTTGATGATGATTTTGAAAATGAAATTACGGGAAGTTTTACCTTTGATACAGATA
AAATTAACTTAGATATAGAAGATGATGATATTAGTGTTTCTTCTTCTCTTTCAGATATATCTACGTCTCCATCTTCTACC
ATATTCACTCCAGAAGTGGAAATTCCTCAAGGAAATTTAGCTAAATACTATAATTTACCTTTACTATCAAAACAGTTAGT
TCATGGTGGTATAACTGGTATTGCTACTTTCTTGATTGTGTTGTTAGTTAGTTTTTCCCAAGCCTCTATTCCTCTAAATA
CGACGAATAAGCCTGAAGTAAAACCGACAACGGAGAAAAAAGAAACTCCGGTTAAACAGGAAGATAAACAAGCCAAAACC
ATTACTTATCCTTTCTTTAACAGATTTTTGTTAAGTTTGATAGCTGGTCTTGCCAGTGGTGGCAGTACAATAGCATTAGG
TTATTTAATGACTAATCATGTTAGACGCTATACCAATGATTTGCAGAATCAATTTGAGTCAGTTTATCAAGGTGATTATG
AAGTTAAAGCAACGGTTTATTCTCAGGATGAATTTGGGACTTTAGCCGCAGGATTTAACCAAATGGCAAAAATGATTAAT
ACTACCACCACAGAAGCCAGAAAACGGGCGGAAGAAACGGAAAGAGCGAGGGAAGATTTACAACGTCAGGTTATTCGTCT
TCTCGATGACGTGGAAGGGGCGGCAAGAGGAGATTTAACGGTACAAGCTGAGGTAACGGCGGACGTTTTAGGGGCGGTGG
CTGATGCTTTTAACCTAACTATTACAAACTTACGGAAAATCGTTAAACAGGTTAAACAAGCGGCGGTTCAAGTAAACAAA
GCGAGTACAGATAGTGAGGTATTCGCCCGAAATCAATCTAGTGATGCGTTGAGGATGGCGGAGGAGTTAGCTGTAACGCT
TAATTCGGTACAAATGATGACTGATTCTATTCAAAGGGTAGCAGAAAACGCTAGGGAAGCGGAAGAAGTTGCTCGATCGT
CTAGTGTTACAGCCCTTAAAGGTGGAGATGCGGTAGAACGAACGGTGGCAGGTATTTTACAAATTAGAGAAACTGTATCG
GAAACGACTCGAAAGGTAAAACGACTAGCGGAAGCATCTCAACAAATTTCCACCATTGTTGCCGTTATTTCTCAAATTTC
CTCTCGTACCAACTTGTTAGCGTTAAATGCTTCGATTCAAGCGGCACGGGCGGGGGAAGCTGGTAGAGGGTTTGCGATCG
TGGCTGATGAAGTACGACAGTTAGCGGATCGTTCGGCTAAATCCTTACAAGAAATTGAACAAATTGTATTACAAATTCAA
ACAGAAACGGGATCGGTAATGACGGCAATGGAAGAGGGTATTCAACAGGTTCGGGATGTAACTGATCGATCGGAACAGGC
AAAACGATCGCTAGAAGATATTATTCAAGTATCTAATCGTATTGATGCTTTAGTTCGATCGATTACTGCTGACACCGATG
AACAAAGAGAAAATTCCAGAGGGGTTGCGAAGGTAATGCAGGCCGTAGAATTAACTGCCCAAGCCACTTCTCAAGAATCC
CAAAGAGTAGCAGGTTCACTACAAAATCTGGTAGGTATTGCGAAAGATCTTATTTCTTCTGTAGAACGTTTTAAAGTAGA
AGGAAAATAA

Domains

Predicted by InterproScan.

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (689-870)

• HAMP domain (HAMP) (515-560)

3D structure

• AlphaFold DB: A0A0D6AP34

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	47.588	100	0.488