Detailed information NTG0066676

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PA257_RS26840
• Genome accession: NZ_AP014651
• Coordinates: 5685540..5686412 (+)
• Length: 290 a.a.
• NCBI ID: WP_003116251.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCGM257
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5680540..5691412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PA257_RS26825	-	5681083..5681781 (-)	699	WP_223677410.1	hypothetical protein	-
PA257_RS34665	-	5681845..5682384 (-)	540	WP_033938465.1	pilin	-
PA257_RS26830 (PA257_5600)	pilB	5682613..5684316 (+)	1704	WP_003116249.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PA257_RS26835 (PA257_5601)	pilC	5684320..5685537 (+)	1218	WP_003116250.1	type II secretion system F family protein	Machinery gene
PA257_RS26840 (PA257_5602)	pilD	5685540..5686412 (+)	873	WP_003116251.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
PA257_RS26845 (PA257_5603)	coaE	5686409..5687020 (+)	612	WP_003094654.1	dephospho-CoA kinase	-
PA257_RS26850 (PA257_5604)	yacG	5687017..5687217 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
PA257_RS26855 (PA257_5605)	-	5687254..5687463 (-)	210	WP_003094660.1	hypothetical protein	-
PA257_RS26860 (PA257_5606)	-	5687569..5688258 (-)	690	WP_003094662.1	energy-coupling factor ABC transporter permease	-
PA257_RS26865 (PA257_5607)	-	5688255..5688725 (-)	471	WP_003094664.1	hypothetical protein	-
PA257_RS26870 (PA257_5608)	-	5688722..5689147 (-)	426	WP_003103865.1	GNAT family N-acetyltransferase	-
PA257_RS26875 (PA257_5609)	-	5689280..5689909 (+)	630	WP_003094668.1	DUF1780 domain-containing protein	-
PA257_RS26880 (PA257_5610)	-	5689906..5690355 (+)	450	WP_023100233.1	MOSC domain-containing protein	-
PA257_RS26885 (PA257_5611)	-	5690381..5690551 (+)	171	WP_003094672.1	DUF3094 family protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31871.89 Da
• Isoelectric Point: 7.3454

>NTDB_id=66676 PA257_RS26840 WP_003116251.1 5685540..5686412(+) (pilD) [Pseudomonas aeruginosa strain NCGM257]
MELLDYLASHPLAFVLCAILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide

• Length: 873 bp

>NTDB_id=66676 PA257_RS26840 WP_003116251.1 5685540..5686412(+) (pilD) [Pseudomonas aeruginosa strain NCGM257]
ATGGAACTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCGCCATCCTCCTTGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAAGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGTTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCGAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (137-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	55.017	99.655	0.548
pilD	Vibrio campbellii strain DS40M4	53.497	98.621	0.528
pilD	Acinetobacter nosocomialis M2	50.352	97.931	0.493
pilD	Acinetobacter baumannii D1279779	50.352	97.931	0.493
pilD	Neisseria gonorrhoeae MS11	49.281	95.862	0.472