Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   MQG43_RS00275 Genome accession   NZ_CP093910
Coordinates   53488..54039 (+) Length   183 a.a.
NCBI ID   WP_005547469.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 27R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 48488..59039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQG43_RS00260 - 49438..50802 (+) 1365 WP_005547462.1 L-serine ammonia-lyase -
  MQG43_RS00265 ansB 50983..52032 (-) 1050 WP_005547464.1 L-asparaginase 2 -
  MQG43_RS00270 - 52306..53466 (+) 1161 WP_102873378.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  MQG43_RS00275 pilF/pilF2 53488..54039 (+) 552 WP_005547469.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  MQG43_RS00280 - 54183..55238 (+) 1056 WP_019518281.1 RodZ family helix-turn-helix domain-containing protein -
  MQG43_RS00285 ispG 55250..56353 (+) 1104 WP_005547472.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  MQG43_RS00290 hisS 56375..57652 (+) 1278 WP_005547474.1 histidine--tRNA ligase -
  MQG43_RS00295 - 57663..58277 (+) 615 WP_005547476.1 YfgM family protein -
  MQG43_RS00300 - 58330..58782 (+) 453 WP_005539334.1 isoprenylcysteine carboxylmethyltransferase family protein -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20667.46 Da        Isoelectric Point: 8.8869

>NTDB_id=665378 MQG43_RS00275 WP_005547469.1 53488..54039(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 27R]
MIKFQQHNRAIPFIFPFLLSACVTQQSAVDFDKQKAAKARVELALGYLQQQDSAQAKLNLDKAFSYAPDYYLVSAAFAYF
YQQQGDIEQARTAYLNAIKLDDKQGDVFNNYGAFLCAQGEFDSAYVQFERALKSSNYYHQADTYENLALCALSAKNQAIY
RQNLALLTKISPKRAAKLPQGIK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=665378 MQG43_RS00275 WP_005547469.1 53488..54039(+) (pilF/pilF2) [Aggregatibacter actinomycetemcomitans strain 27R]
ATGATCAAATTTCAACAGCACAATCGTGCAATACCGTTTATTTTTCCGTTTTTGCTTTCCGCCTGCGTCACCCAACAAAG
TGCGGTGGATTTTGACAAGCAAAAAGCCGCCAAAGCACGCGTGGAACTGGCATTAGGCTATTTACAGCAGCAAGATTCGG
CACAAGCCAAGCTGAACCTGGATAAAGCCTTTTCTTACGCTCCCGATTATTATTTAGTGTCAGCGGCATTTGCCTATTTC
TATCAACAACAGGGCGACATTGAACAAGCCCGTACCGCCTATTTAAACGCGATAAAATTAGATGATAAACAAGGGGATGT
GTTCAATAATTACGGTGCATTTCTTTGTGCGCAAGGGGAATTCGACAGTGCCTATGTACAATTTGAACGCGCCTTAAAAA
GCTCGAATTATTACCATCAGGCGGACACTTATGAAAACTTGGCGTTATGTGCTTTATCCGCCAAAAATCAGGCGATTTAT
CGGCAAAATCTCGCATTATTGACGAAAATCTCTCCGAAACGGGCGGCAAAATTACCACAAGGGATAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(78-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.576

90.164

0.519
  pilF Glaesserella parasuis strain SC1401

47.059

92.896

0.437