Detailed information NTG0066530

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: NCGM1984_RS28620
• Genome accession: NZ_AP014646
• Coordinates: 6134318..6135382 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain NCGM1984
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6129318..6140382

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NCGM1984_RS28595 (NCGM1984_5692)	aroK	6129853..6130371 (-)	519	WP_003095833.1	shikimate kinase AroK	-
NCGM1984_RS28600 (NCGM1984_5693)	pilQ	6130383..6132527 (-)	2145	WP_003095835.1	type 4a pilus secretin PilQ	Machinery gene
NCGM1984_RS28605 (NCGM1984_5694)	pilP	6132581..6133105 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	-
NCGM1984_RS28610 (NCGM1984_5695)	pilO	6133102..6133725 (-)	624	WP_003095838.1	type 4a pilus biogenesis protein PilO	-
NCGM1984_RS28615 (NCGM1984_5696)	pilN	6133722..6134318 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	-
NCGM1984_RS28620 (NCGM1984_5697)	pilM	6134318..6135382 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
NCGM1984_RS28625 (NCGM1984_5698)	-	6135567..6138038 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
NCGM1984_RS28630 (NCGM1984_5699)	-	6138134..6139402 (-)	1269	WP_003095842.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=66530 NCGM1984_RS28620 WP_003110888.1 6134318..6135382(-) (pilM) [Pseudomonas aeruginosa strain NCGM1984]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=66530 NCGM1984_RS28620 WP_003110888.1 6134318..6135382(-) (pilM) [Pseudomonas aeruginosa strain NCGM1984]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCTTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGTTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466