Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   MNO09_RS30420 Genome accession   NZ_CP093325
Coordinates   5422533..5423909 (-) Length   458 a.a.
NCBI ID   WP_001085205.1    Uniprot ID   A0A2A8XI58
Organism   Bacillus sp. N5-665     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5417533..5428909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO09_RS30400 (MNO09_30220) ispF 5418903..5419379 (-) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  MNO09_RS30405 (MNO09_30225) ispD 5419495..5420175 (-) 681 WP_000288298.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  MNO09_RS30410 (MNO09_30230) - 5420192..5421295 (-) 1104 WP_000919687.1 PIN/TRAM domain-containing protein -
  MNO09_RS30415 (MNO09_30235) disA 5421456..5422529 (-) 1074 WP_000392165.1 DNA integrity scanning diadenylate cyclase DisA -
  MNO09_RS30420 (MNO09_30240) radA 5422533..5423909 (-) 1377 WP_001085205.1 DNA repair protein RadA Machinery gene
  MNO09_RS30425 (MNO09_30245) clpC 5424006..5426441 (-) 2436 WP_000971181.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  MNO09_RS30430 (MNO09_30250) - 5426464..5427528 (-) 1065 WP_000050830.1 protein arginine kinase -
  MNO09_RS30435 (MNO09_30255) - 5427533..5428081 (-) 549 WP_000128403.1 UvrB/UvrC motif-containing protein -
  MNO09_RS30440 (MNO09_30260) ctsR 5428254..5428715 (-) 462 WP_001244576.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49874.55 Da        Isoelectric Point: 7.4250

>NTDB_id=663630 MNO09_RS30420 WP_001085205.1 5422533..5423909(-) (radA) [Bacillus sp. N5-665]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVKGSNLFVVAETDLQRIA
AHIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTTPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=663630 MNO09_RS30420 WP_001085205.1 5422533..5423909(-) (radA) [Bacillus sp. N5-665]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGGAAATGCCCTGGTTG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGACGCCTTAATTATGCCAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAATCTGAGGCACGTATTGAAACGAAATTCCAAGAGTTT
AATCGTGTACTTGGTGGTGGGATTGTAGATGGATCCTTAGTGCTTATTGGTGGAGACCCTGGGATTGGGAAATCAACATT
GTTATTACAAATTTCATCGCAATTAGCAGATTCTTCATATGATGTACTATACATATCAGGTGAGGAGTCAGCAAAGCAGA
TTAAACTTCGTGCAGATCGTTTGCATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAAACTGATCTACAGCGAATTGCA
GCGCACATTGAAGAGATGAATCCGGCTTTTGTTGTTATTGACTCTATTCAAACGATACATTTACCTGAAGTGACGTCAGC
CCCAGGAAGTGTAGCGCAAGTACGTGAATGTACAGCGGAACTAATGAAACTTGCAAAAACGAAAGGGATTCCTATTTTTA
TCGTCGGACATGTAACAAAAGAGGGGGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGATCGTCATCATACATATCGTATTTTGCGAGCTGTGAAGAATCGTTTTGGTTCCACGAATGAAATGGGTATTTT
TGAAATGAAAGAGCTTGGTCTTGCGGAAGTCTTAAACCCTTCTGAAATTTTTCTTGAGGAAAGACCCGTTGGAGTCGCAG
GATCAACAGTAGTTGCCTCAATGGAAGGAACAAGACCGGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCGACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCAGTGCTAGAAAAAAGAACAGG
TTTATTATTGCAAAACCAAGACGCATATTTAAAAGTAGCAGGTGGCTTGAAATTAGATGAACCAGCAATTGATTTAGCTG
TGGCTTTAAGTATTGCTTCAAGTTTTAGAGATAAATCTACGACACCAACTGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGGGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGGGGATGGACAATTCCGGATGGGATTGAGGTAGTAGGTGTATCTAATTTAGGGGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(60-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A8XI58

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.51

100

0.747
  radA Streptococcus pneumoniae Rx1

64.018

98.908

0.633
  radA Streptococcus pneumoniae D39

64.018

98.908

0.633
  radA Streptococcus pneumoniae R6

64.018

98.908

0.633
  radA Streptococcus pneumoniae TIGR4

64.018

98.908

0.633
  radA Streptococcus mitis SK321

64.018

98.908

0.633
  radA Streptococcus mitis NCTC 12261

63.797

98.908

0.631