Detailed information NTG0663623

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: MNO09_RS25170
• Genome accession: NZ_CP093325
• Coordinates: 4391109..4394834 (-)
• Length: 1241 a.a.
• NCBI ID: WP_000572284.1
• Uniprot ID: A0AB37YMN8
• Organism: Bacillus sp. N5-665
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4386109..4399834

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MNO09_RS25120 (MNO09_24980)	-	4387003..4387287 (+)	285	WP_000926860.1	hypothetical protein	-
MNO09_RS25125 (MNO09_24985)	-	4387322..4388221 (-)	900	WP_001241733.1	fumarylacetoacetate hydrolase family protein	-
MNO09_RS25130 (MNO09_24990)	-	4388468..4388647 (+)	180	WP_000462847.1	aspartyl-phosphate phosphatase Spo0E family protein	-
MNO09_RS25135 (MNO09_24995)	gerPA	4388743..4388964 (+)	222	WP_001111187.1	spore germination protein GerPA	-
MNO09_RS25140 (MNO09_25000)	gerPB	4388979..4389185 (+)	207	WP_001055456.1	spore germination protein GerPB	-
MNO09_RS25145 (MNO09_25005)	gerPC	4389254..4389868 (+)	615	WP_001070745.1	spore germination protein GerPC	-
MNO09_RS25150 (MNO09_25010)	gerPD	4389875..4390069 (+)	195	WP_001102341.1	spore germination protein GerPD	-
MNO09_RS25155 (MNO09_25015)	-	4390085..4390471 (+)	387	WP_000902339.1	spore germination protein GerPE	-
MNO09_RS25160 (MNO09_25020)	gerPF	4390514..4390729 (+)	216	WP_001141570.1	spore germination protein GerPF	-
MNO09_RS25165 (MNO09_25025)	-	4390809..4391096 (-)	288	WP_000447115.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
MNO09_RS25170 (MNO09_25030)	addA	4391109..4394834 (-)	3726	WP_000572284.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MNO09_RS25175 (MNO09_25035)	addB	4394831..4398346 (-)	3516	WP_000058581.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MNO09_RS25180 (MNO09_25040)	lepB	4398463..4399026 (-)	564	WP_000751908.1	signal peptidase I	-
MNO09_RS25185 (MNO09_25045)	-	4399224..4399817 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142599.81 Da
• Isoelectric Point: 4.8847

>NTDB_id=663623 MNO09_RS25170 WP_000572284.1 4391109..4394834(-) (addA) [Bacillus sp. N5-665]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNSIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEAHI
RKATELAMFPDGPAPRVETLQADAVLLGTLSSAACESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVQDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLQWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPGEIYGYDTSWK
VEVVDGKTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVHNRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKQPITEEVIREQIAGMVNKELLTFEQAEEVAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKGDETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=663623 MNO09_RS25170 WP_000572284.1 4391109..4394834(-) (addA) [Bacillus sp. N5-665]
ATGATTGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACGGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATATTAGTCGCAGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCACTAGAAAAAGTATTAATTGATGAGCCAGGATCGCAGCACGTAAGAAAGCAGCTTAGCTTATTAAATAAAGC
TTCTATTTCGACGATCCATTCCTTTTGTTTACAAGTAATTAGAGGATACTATTACATGCTTGATGTGGATCCTCGTTTCC
GCATTGCGAATCAAACGGAAAATGAACTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAAGAGTACGGAATTGAA
GATAATAGTATTTTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAGCGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATACGACGTGGAAGGAA
AGACAATTGAAGATTTAGTGTATGCTTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCAGAGGCACATATT
CGTAAAGCGACTGAACTCGCAATGTTTCCTGACGGTCCGGCGCCCCGCGTTGAAACCCTGCAAGCAGATGCAGTTTTACT
TGGAACGTTGTCATCAGCTGCTTGTGAGTCGTGGACAAGTGTGTATGAAGCGATGCAAAATGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGTTATTTAGCCGCAGACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAACTCGTACAGCTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAACGGTGAAATGAAGCCGTCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTTGTGCAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTAGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGACTATTCTTAGGAAAGTATAAACGCTTTACGCAAGAAGGATTAGGCGGCGGAATGAAGATCGATTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTCGGAGAAAT
CGACTACGATGCTGACGCTGAATTAAAGTTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGTA
TTCAGCAAACAGAAGAAGAAGTGCAAGACGGAGAAGAAGGTGCGGAAGTAGAAAAAGCACAACTTGAAGCTCGTCTTATG
GCGCAGCGTATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCTATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTGATCGATAAT
CCGATGCAAGATATTCCGCTTGCCGCCGTACTTCGTTCACCAATCGTTGGACTAAATGATGAAGAACTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAT
TACATGATAAATTACAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTCGCGCGTCAACAGTCACTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTACGATTTTGTCGGCGGTTTACCAGCCGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTTTATGACCGTGCAAGACAATATGAAGCAACATCGTTTAGAGGATTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGAACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGTATTATGACGATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTTGTAGCGGGACTAGGTCGTCGTTTTAATACACAAGATTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCACAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTAGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTGTTATACGTAGCGTTAACGCGT
GCGAAAGAGAAGTTAATTTTAATTGGAACGGTTAAAGATGCCAATAAAGAAATGGAAAAATGGCTCGATGCAAGGGAGCA
TAGTGAATGGTTATTACCAGACCATATACGTGCCGGAGCGTCCTGTTATTTAGATTGGATTGCACCTTCATTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGCATTCCGGGTGAAATTTATGGATATGACACGAGCTGGAAA
GTAGAAGTTGTTGACGGCAAAACGTTACTTGCACCAGAACCGGTTCAAGAAGAGAAACAAGAATTACTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCGTTAGAAAGTGAACGAAAAGAAGAAGTGCATAACAGATTAATGTGGAAGTACGGATATGAGG
AAGCAACATCTCACCGTGCGAAACAGTCTGTGACAGAAATAAAGAGAAACTACCAATCAGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTGCGTGCACCAATTAAAACGCGTCCTCGTTTTATGGAAAAAAAAGGATTAACGTATGCTGAAAGAGG
TACGGCGGTTCACGCTGTTATGCAGCATGTTGATTTGAAGCAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCCG
GAATGGTAAATAAGGAATTATTAACATTCGAACAAGCTGAAGAAGTAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAGAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAGGC
GTATCAAGATTGGCAAGGTAAGGGTGACGAAACGATACTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATTGATTTTAAAACGGATACGATTGAAGGAAAGTTCCCAGGTGGATTCGATCAAGCAAAACCAATTTTA
GAAGATCGATACAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAAAAAAGCTTACAACATCCTGTGAAAGAGAAATG
CTTATACTTCTTTGATGGCAATCATGTTGTAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534