Detailed information NTG0663624

Bioinformatically predicted

Overview

• Name: addB
• Type: Machinery gene
• Locus tag: MNO09_RS25175
• Genome accession: NZ_CP093325
• Coordinates: 4394831..4398346 (-)
• Length: 1171 a.a.
• NCBI ID: WP_000058581.1
• Uniprot ID: -
• Organism: Bacillus sp. N5-665
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4389831..4403346

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MNO09_RS25150 (MNO09_25010)	gerPD	4389875..4390069 (+)	195	WP_001102341.1	spore germination protein GerPD	-
MNO09_RS25155 (MNO09_25015)	-	4390085..4390471 (+)	387	WP_000902339.1	spore germination protein GerPE	-
MNO09_RS25160 (MNO09_25020)	gerPF	4390514..4390729 (+)	216	WP_001141570.1	spore germination protein GerPF	-
MNO09_RS25165 (MNO09_25025)	-	4390809..4391096 (-)	288	WP_000447115.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
MNO09_RS25170 (MNO09_25030)	addA	4391109..4394834 (-)	3726	WP_000572284.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
MNO09_RS25175 (MNO09_25035)	addB	4394831..4398346 (-)	3516	WP_000058581.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
MNO09_RS25180 (MNO09_25040)	lepB	4398463..4399026 (-)	564	WP_000751908.1	signal peptidase I	-
MNO09_RS25185 (MNO09_25045)	-	4399224..4399817 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-
MNO09_RS25190 (MNO09_25050)	-	4400206..4400787 (-)	582	WP_000444424.1	competence protein ComK	-
MNO09_RS25195 (MNO09_25055)	-	4401071..4401256 (+)	186	WP_000391440.1	hypothetical protein	-
MNO09_RS25200 (MNO09_25060)	cspA	4401509..4401712 (-)	204	WP_000301522.1	RNA chaperone/antiterminator CspA	-
MNO09_RS25205 (MNO09_25065)	-	4402087..4402878 (+)	792	WP_088106942.1	phosphotransferase	-

Sequence

Protein

• Length: 1171 a.a.
• Molecular weight: 134381.49 Da
• Isoelectric Point: 5.1786

>NTDB_id=663624 MNO09_RS25175 WP_000058581.1 4394831..4398346(-) (addB) [Bacillus sp. N5-665]
MSLRFVIGRAGSGKSTLCLHEVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKEGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEYVNGAEIYIDGFHSFSPQELEILRQLMICGARVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSQALAHLETHYEARPNEKFHGEASVTISTAANL
RAEVEGVAREIRRLVADEDYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKETMREEMDEFENYCLAYGVQGKRWTSEDPWMYRRYRSLDDTDEMITDSEREMEERINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEALRIRAEESGDFLFATDHEQVWEEVMSLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDRSRLSNVKATFVIGVNEGVIPAAPMDEGMLSDEERDVLSVAGIEL
APTTRQTLLEEQFVMYQMVTRATEKLYISCPLADEEGKTLLASSFIKKVKRMFPDVKDTFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTVVSRDLYGDKIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKRIADRLLRENRTWADLSIKECEHLSAVVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAHTWMKKGGTASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKKGNKAACTFCNFKSVCQFDESLEDNQFRTLKDMKDSEAMEKIREEVGGE

Nucleotide

• Length: 3516 bp

>NTDB_id=663624 MNO09_RS25175 WP_000058581.1 4394831..4398346(-) (addB) [Bacillus sp. N5-665]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACACGAAGTGCAGGAAGAGTTAAA
GCAACGCCCAAGAGGGAAGACAATATTATATCTTGTGCCAGAACAGATGACATTCCAAACGCAGCAGGCGTTAATCGGGA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTCGGC
GGAGCGAGTCGTCTTCACATTGATGAAGCGGGCGTACATATGTTGCTTCGTAAAATTGTTGAGTCTCGTAAAGAGGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGTAGTATGATTGCGGAGTTTAAACGTTACA
ATGTGACGCCATCTAACGTATATGAAATGTGGCAACAATTAGACGCACATAGTAGCAGTGCAGAACAAAAGCTATTAGCG
AATAAAGTGTATGATTTACAACTATTATATGATGATTTTGAACGTGCTTTAATCGGAAAATATTTGGATTCAGAAGACTA
CCTACAATTGTTAGTCGAAAAGCTTCCACAGTCTGAATATGTAAATGGGGCTGAAATTTATATAGATGGATTTCATTCGT
TCTCACCGCAAGAACTAGAAATTTTAAGACAGCTTATGATTTGCGGCGCGAGAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCGCAGCCAGTAAATGAACTAGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAGCAAGTGGC
GCGTGAAGAGAAGATAGAAATTGAAAAAACAATTCCACTTATGGAACAACCGCGCTTTCATTCGCAAGCATTAGCGCATT
TAGAAACTCATTACGAAGCACGTCCAAATGAAAAGTTTCACGGAGAAGCAAGTGTAACGATTAGTACAGCAGCGAATTTA
CGAGCTGAAGTAGAAGGTGTTGCGCGTGAAATTCGTAGACTTGTGGCGGATGAAGACTATCGTTACCGAGATATTGCAGT
CCTTCTTCGTAACGGAGAAAGTTATTACGATGTGATGCGAACGCTATTTACAGATTATAATATCCCGCACTTCATCGATG
AAAAACGCCCGATGTCACATCATCCGCTAGTAGAATGCATTCGTTCTGCACTCGAGATTATTAGCGGAAATTGGCGTTAT
GATGCAGTGTTCCGCTGCGTGAAAACAGAGCTTTTATATCCACTAGACGTAAGAAAAGAAACGATGCGCGAAGAGATGGA
TGAGTTTGAAAACTACTGTTTAGCGTACGGTGTACAAGGGAAGAGATGGACTTCTGAGGATCCGTGGATGTATCGCCGCT
ATCGTTCTCTTGACGATACGGACGAGATGATTACAGACAGTGAACGTGAAATGGAAGAGAGAATAAATCGACTGCGCGAC
GTTGTAAGAACGCCTGTTATTCGTATGCAAAAAAGACTGAAGCGAGCGGGAACAGTTATGCAAATGTGCGAAGCTGTTTA
TTTATTTTTAGAAGAGCTAGACGTTCCAAAAAAACTAGAAGCATTACGTATTCGTGCAGAAGAGAGCGGAGATTTCTTAT
TTGCGACAGATCATGAGCAAGTATGGGAAGAAGTAATGAGTCTTCTTGATACATTCGTTGAGATGCTTGGCGAAGAGAAA
ATGTCACTTTCTATGTTCACAGATGTTATGTCGACAGGTCTTGAGGCGCTTCAATTTGCAAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCGTTCAAGATTATCAAATGTGAAAGCAACATTTGTCATTGGCGTGAATGAAGGAG
TTATTCCAGCAGCACCGATGGATGAAGGGATGCTTTCTGATGAAGAAAGAGATGTTCTTAGCGTTGCAGGTATCGAATTA
GCACCAACGACGAGACAAACCTTATTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCAACTGAGAAATTATA
TATTTCATGCCCGCTTGCAGATGAAGAAGGGAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAGTAAAAAGAATGTTCC
CTGATGTGAAAGATACATTTATTACAAATGACGTAAATGATTTATCACGTTCGGAACAAATTTCATATGTAGCAACGCCA
GAAGTAACGTTGTCATATGTTATGCAGCAACTTCAAACGTGGAAGCGATATGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAACTTCTACGTAACTTCAGATGAATGGAAACAAAAAAGTAGCCGCGTATTATCAAGTTTATTCTACCGAA
ATCGTGCGCAAAAGCTAAGTACAGTTGTAAGTAGAGATTTATACGGAGATAAAATAAAAGGAAGCGTATCTCGTATGGAA
CTATTTAACCGTTGCGCATATGCTCATTTCGCGCAGCACGGTTTATCCTTAAGAGAGCGTGATATTTTCAAACTGGATGC
GCCAGATATCGGGGAACTATTCCATGCAGCGCTGAAGAGAATTGCGGACAGGCTATTACGTGAAAACCGTACTTGGGCGG
ATTTATCAATAAAAGAGTGTGAGCATCTTTCGGCTGTAGTAATAGAAGAGATTGCACCGTTATTACAAAGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATCATTTTCCGTACATCAATCATTCTTCGTGAACA
TGCGAAGTCAAGCGGTTTCGTACCAGTTGATTTAGAAGTGCCATTCGGTATGGGCGGTACAGGATCGCTTCCGCCGATGG
AATTCTCATTACCAAATGGTGTAAAGATGGAAGTAGTTGGTCGTATTGACCGTGTTGATAAGGCGGAAGATGAAAACGGA
ACGTTCCTACGTATTATTGACTATAAATCAAGCTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCAAATGCACATACGTGGATGAAAAAAGGCGGCACGGCATCACCGGCTGGTG
TACTGTACTTCCATATTCATAACCCGATCGTTGAGATGAAAGGTGACGCATCTGAAGCAGAAATTGAGAAAGAGATTTTA
AAGAAATTTAAAATGAAAGGTCTCGTACTAGGAGACGCTGATGTTGTGCGTTTAATGGATAACAAACTTTCAACAGGAAG
TTCTGATATTATTTCTGCTGGTTTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGCATTGCAAGTGAACAAGAGTTTA
ACGTACTGCAAAAATACGTACACCATACGTTTGAAAATATCGGAAAAGACATTACAGAAGGCGTTATTGATATTGCTCCT
TATAAGAAGGGGAATAAAGCGGCGTGTACGTTCTGTAACTTCAAATCTGTTTGTCAGTTCGATGAATCACTTGAAGATAA
CCAGTTCCGTACGCTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATTAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (302-669)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (795-1139)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addB	Bacillus subtilis subsp. subtilis str. 168	49.319	100	0.494