Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MNO09_RS08560 Genome accession   NZ_CP093325
Coordinates   1193248..1194291 (+) Length   347 a.a.
NCBI ID   WP_033657726.1    Uniprot ID   -
Organism   Bacillus sp. N5-665     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1188248..1199291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNO09_RS08525 (MNO09_08450) - 1188428..1188916 (+) 489 WP_000764275.1 hypothetical protein -
  MNO09_RS08530 (MNO09_08455) - 1188955..1189266 (-) 312 WP_001093232.1 hypothetical protein -
  MNO09_RS08535 (MNO09_08460) - 1189521..1189736 (+) 216 WP_001008334.1 DUF3912 family protein -
  MNO09_RS08540 (MNO09_08465) - 1189835..1190242 (+) 408 WP_000875386.1 hypothetical protein -
  MNO09_RS08545 (MNO09_08470) - 1190476..1191870 (-) 1395 WP_001094342.1 L-cystine transporter -
  MNO09_RS08550 (MNO09_08475) - 1191977..1192219 (-) 243 WP_000440714.1 DUF2626 domain-containing protein -
  MNO09_RS08555 (MNO09_08480) - 1192346..1193041 (-) 696 WP_000434122.1 helix-turn-helix domain-containing protein -
  MNO09_RS08560 (MNO09_08485) comGA 1193248..1194291 (+) 1044 WP_033657726.1 competence protein ComGA Machinery gene
  MNO09_RS08565 (MNO09_08490) comGB 1194284..1195315 (+) 1032 WP_000471915.1 competence type IV pilus assembly protein ComGB -
  MNO09_RS08570 (MNO09_08495) comGC 1195327..1195626 (+) 300 WP_001178693.1 competence type IV pilus major pilin ComGC -
  MNO09_RS08575 (MNO09_08500) comGD 1195623..1196078 (+) 456 WP_000810391.1 comG operon protein ComGD -
  MNO09_RS08580 (MNO09_08505) - 1196071..1196373 (+) 303 WP_000829519.1 hypothetical protein -
  MNO09_RS08585 (MNO09_08510) comGF 1196343..1196813 (+) 471 WP_000923107.1 competence type IV pilus minor pilin ComGF -
  MNO09_RS08590 (MNO09_08515) comGG 1196810..1197181 (+) 372 WP_001231189.1 competence type IV pilus minor pilin ComGG -
  MNO09_RS08595 (MNO09_08520) - 1197357..1198007 (+) 651 WP_000183839.1 2OG-Fe(II) oxygenase -
  MNO09_RS08600 (MNO09_08525) - 1198126..1198623 (+) 498 WP_000836644.1 shikimate kinase -
  MNO09_RS08605 (MNO09_08530) - 1198662..1198862 (+) 201 WP_000106080.1 YqzE family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39306.91 Da        Isoelectric Point: 8.7272

>NTDB_id=663603 MNO09_RS08560 WP_033657726.1 1193248..1194291(+) (comGA) [Bacillus sp. N5-665]
MNGIEIFANTILKEACRVQASDLHIVPRKKDVAVQLRIGKDLMTKHCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLHYSHGLLVFTGPTGSGKTTTMYALLEV
IKKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TSDAKGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCQRKCSILCKSMREVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLESLIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=663603 MNO09_RS08560 WP_033657726.1 1193248..1194291(+) (comGA) [Bacillus sp. N5-665]
ATGAATGGAATTGAAATTTTTGCGAATACGATTTTGAAAGAGGCGTGCAGGGTACAAGCTTCGGATTTACATATTGTGCC
CCGGAAGAAGGATGTAGCGGTTCAACTGCGTATAGGAAAAGATTTAATGACGAAACACTGTATCGAAAAGGAATTTGGAG
AAAAGCTTGTTTCACACTTTAAATTTTTAGCATCCATGGATATAGGGGAGAGACGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTACGCCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAAAAACTACTCTCTTTTTTAC
ACTATTCACATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAAAAAAAAGAAAACACGTCGCATCGTTACACTTGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCGGGACTAAAGGCCATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATCGCTATAAGAGCAAGTTTGACTGGACATTTAGTAATGACGACACTGCAT
ACTAGTGATGCGAAAGGGGCGATACTCCGATTTATGGATTTCGGTATAACGAGGCAAGAGATTGAACAATCTTTATTGGC
TATAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCCAAAGAAAGTGCTCAATTTTATGTAAATCAATGAGGG
AGGTAAGACAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCATGAAACATTAGAATCTTTGATACGAAAAGGATACGCTTTAGGATTTTTAGAAGAGGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.501

100

0.585
  pilF Thermus thermophilus HB27

38.58

93.372

0.36