Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   MNU35_RS00325 Genome accession   NZ_CP093170
Coordinates   72681..74309 (-) Length   542 a.a.
NCBI ID   WP_254106263.1    Uniprot ID   -
Organism   Staphylococcus epidermidis strain TMDU-128     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
SCCmec 37294..75680 72681..74309 within 0


Gene organization within MGE regions


Location: 37294..75680
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MNU35_RS00155 (MNU35_00155) mecA 37294..39303 (-) 2010 WP_063852638.1 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA -
  MNU35_RS00160 (MNU35_00160) - 39400..39507 (+) 108 Protein_29 beta-lactam sensor/signal transducer MecR1 -
  MNU35_RS00165 (MNU35_00165) - 39548..40222 (+) 675 Protein_30 IS6 family transposase -
  MNU35_RS00170 (MNU35_00170) - 40282..40710 (-) 429 WP_000671996.1 VOC family protein -
  MNU35_RS00175 (MNU35_00175) - 40791..41720 (+) 930 WP_044466476.1 HTH domain-containing protein -
  MNU35_RS00180 (MNU35_00180) - 41882..43894 (+) 2013 WP_162158236.1 hypothetical protein -
  MNU35_RS00185 (MNU35_00185) - 44064..45173 (+) 1110 WP_049319120.1 DNA polymerase -
  MNU35_RS00190 (MNU35_00190) - 45166..45537 (+) 372 WP_002437511.1 hypothetical protein -
  MNU35_RS00195 (MNU35_00195) - 45534..47153 (+) 1620 WP_002437513.1 DUF5906 domain-containing protein -
  MNU35_RS00200 (MNU35_00200) - 47380..49059 (+) 1680 WP_099774654.1 recombinase family protein -
  MNU35_RS00205 (MNU35_00205) - 49148..49486 (+) 339 WP_017175172.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU35_RS00210 (MNU35_00210) - 49580..49891 (+) 312 WP_001166716.1 DUF960 family protein -
  MNU35_RS00215 (MNU35_00215) - 49907..50413 (+) 507 WP_000848380.1 DUF1643 domain-containing protein -
  MNU35_RS00220 (MNU35_00220) - 50434..50880 (+) 447 WP_049389704.1 RadC family protein -
  MNU35_RS00225 (MNU35_00225) - 51050..51922 (+) 873 WP_000373151.1 HTH domain-containing protein -
  MNU35_RS00230 (MNU35_00230) - 51979..52269 (+) 291 WP_000065262.1 hypothetical protein -
  MNU35_RS00235 (MNU35_00235) - 52285..52773 (+) 489 WP_000397419.1 hypothetical protein -
  MNU35_RS00240 (MNU35_00240) - 52908..53435 (+) 528 WP_099774652.1 hypothetical protein -
  MNU35_RS00245 (MNU35_00245) - 53497..53877 (+) 381 WP_254106258.1 hypothetical protein -
  MNU35_RS00250 (MNU35_00250) - 53885..54106 (+) 222 WP_002504703.1 hypothetical protein -
  MNU35_RS00255 (MNU35_00255) - 54260..54826 (+) 567 WP_099774648.1 PepSY domain-containing protein -
  MNU35_RS00260 (MNU35_00260) - 54904..58023 (-) 3120 WP_099774646.1 type I restriction endonuclease subunit R -
  MNU35_RS00265 (MNU35_00265) - 58007..59269 (-) 1263 WP_099774644.1 restriction endonuclease subunit S -
  MNU35_RS00270 (MNU35_00270) - 59259..60773 (-) 1515 WP_145382875.1 class I SAM-dependent DNA methyltransferase -
  MNU35_RS00275 (MNU35_00275) - 61202..61921 (+) 720 WP_032604338.1 hypothetical protein -
  MNU35_RS00280 (MNU35_00280) - 61918..62814 (-) 897 WP_002437539.1 hypothetical protein -
  MNU35_RS00285 (MNU35_00285) - 63113..66385 (-) 3273 WP_254106259.1 N-6 DNA methylase -
  MNU35_RS00290 (MNU35_00290) - 66466..66873 (-) 408 WP_096638061.1 hypothetical protein -
  MNU35_RS00295 (MNU35_00295) - 67126..67488 (-) 363 WP_254106260.1 hypothetical protein -
  MNU35_RS12345 - 67533..67736 (-) 204 WP_367269962.1 hypothetical protein -
  MNU35_RS00300 (MNU35_00300) - 67872..68375 (-) 504 WP_254106261.1 DUF1643 domain-containing protein -
  MNU35_RS00305 (MNU35_00305) - 68390..68701 (-) 312 WP_070863099.1 DUF960 domain-containing protein -
  MNU35_RS00310 (MNU35_00310) - 68794..69135 (-) 342 WP_145378659.1 SAUGI family uracil-DNA glycosylase inhibitor -
  MNU35_RS00315 (MNU35_00315) - 69744..70427 (-) 684 WP_145378657.1 M15 family metallopeptidase -
  MNU35_RS00320 (MNU35_00320) pbp4 70654..71898 (+) 1245 WP_254106262.1 penicillin-binding protein PBP4 -
  MNU35_RS00325 (MNU35_00325) ccrB 72681..74309 (-) 1629 WP_254106263.1 recombinase family protein Machinery gene
  MNU35_RS00330 (MNU35_00330) ccrA 74331..75680 (-) 1350 WP_254106264.1 recombinase family protein Machinery gene

Sequence

Protein


Download         Length: 542 a.a.        Molecular weight: 62628.87 Da        Isoelectric Point: 9.7869

>NTDB_id=662092 MNU35_RS00325 WP_254106263.1 72681..74309(-) (ccrB) [Staphylococcus epidermidis strain TMDU-128]
MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFEVKDIYADRGISGKSMNRPELQHMLKDAKEGNIDCVM
VYKTNRLARNTSDLLKIVEDLHKQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
LPLGYDKIPDSKHELMINQHEANIVKYIFESYAKGHGYRKIANALNHKGYVTKKGKPFSISSITYILTNPFYIGKIQFAK
YKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQMRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKD
GTKKRIRYYSCSNFRNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVARVNQENQVDCASLHHDIAYKQQQYD
EVQIKLNNLIKTIEDNPDLTSVIRPSIQKYDKQLNDIMSQINQLKKQQNEDKPSYNAKKIGEILRHVFHNIEQMKKSQLK
ALYLAVIDRIDIRKDSNHKKQFYVTLKLDNEVIKQVFNDIPLDEVLLSTSSLFLPQPFYLTI

Nucleotide


Download         Length: 1629 bp        

>NTDB_id=662092 MNU35_RS00325 WP_254106263.1 72681..74309(-) (ccrB) [Staphylococcus epidermidis strain TMDU-128]
ATGCAACAACTTAAAACAAAACGTGTCGGTATCTATGTACGTGTATCAACAGAAATGCAAAGCACAGAAGGTTATAGTAT
CGACGGACAAATCAATCAAATCAAAGAATACTGTGACTTCCATCATTTTGAGGTTAAAGATATATACGCTGACCGTGGTA
TTTCAGGTAAATCTATGAATCGACCTGAGCTCCAACATATGTTGAAAGATGCAAAAGAAGGCAATATCGACTGTGTTATG
GTCTACAAAACAAACCGATTAGCTCGTAATACATCTGATCTTCTCAAAATTGTCGAAGATTTACACAAACAAAATGTCGA
ATTTTTTAGTTTGTCAGAGCGTATGGAAGTCAATACTTCTTCTGGTAAACTCATGTTACAGATACTTGCGAGTTTCTCAG
AATTCGAACGTAATAACATTGTCGAGAACGTATTTATGGGGCAAACGAGACGTGCCCAAGAAGGCTATTATCAAGGCAAT
TTACCACTAGGTTATGACAAAATACCAGATAGTAAGCACGAGCTAATGATTAACCAACATGAAGCTAATATTGTAAAATA
TATATTCGAGTCCTATGCCAAAGGACATGGCTATCGTAAAATAGCCAATGCATTGAATCACAAAGGCTATGTCACTAAAA
AGGGTAAGCCTTTTAGTATTAGTTCTATCACATATATATTAACTAACCCATTCTATATCGGCAAAATTCAATTTGCGAAA
TACAAAGATTGGAGTGAAAAACGTCGTAAAGGGCTGAATGATAAACCAGTGATAGCTGAAGGTAAGCATTCCCCCATTAT
TAATCAAGATTTATGGGATAAAGTACAAATGCGTAAAAAACAAGTCAGTCAAAAACCCCAAGTTCATGGTAAAGGAACGA
ATCTGCTTACAGGCATTATCCACTGTCCCCAATGTGGCGCACCTATGGCAGCAAGCAATACAACGAATACACTTAAAGAT
GGAACCAAGAAACGCATACGTTACTATTCATGTAGTAATTTTCGTAACAAGGGGTCCAAAGTATGTTCGGCAAACAGTGT
AAGAGCTGATGTGATTGAAGATTATGTGATGAAGCAAATACTCGAAATAGTCAAAAGTGATAAGGTCATTCAACGCGTTG
TAGCACGTGTTAATCAAGAAAATCAAGTTGATTGTGCGTCACTTCATCACGATATCGCTTATAAGCAACAACAATATGAT
GAAGTACAAATCAAACTAAATAATCTGATTAAAACCATCGAGGATAATCCGGACTTAACATCAGTAATCAGGCCGTCGAT
TCAAAAATATGATAAGCAACTTAATGACATTATGAGTCAAATCAATCAACTCAAAAAACAACAAAATGAAGATAAACCCT
CATATAATGCCAAAAAAATTGGTGAAATATTGCGACACGTCTTTCATAATATTGAGCAAATGAAAAAATCTCAACTTAAA
GCACTGTACTTAGCAGTGATTGATCGCATTGATATTAGAAAAGATAGCAATCACAAAAAACAGTTTTATGTAACACTCAA
ACTTGATAATGAAGTAATTAAACAGGTTTTCAATGATATTCCTCTCGACGAAGTGCTCCTTAGCACTTCGTCTTTATTTT
TACCCCAACCTTTTTATTTAACAATATAA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(11-156)

Ogr/Delta-like zinc finger (Ogr_Delta)

(301-356)

Recombinase (Recombinase)

(180-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

92.62

100

0.926