Detailed information NTG0661281

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MMY94_RS23800
• Genome accession: NZ_CP093010
• Coordinates: 4973703..4974500 (+)
• Length: 265 a.a.
• NCBI ID: WP_032694399.1
• Uniprot ID: A0A2J5PD60
• Organism: Klebsiella michiganensis strain K342
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4968703..4979500

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MMY94_RS23765 (MMY94_23765)	gspH	4968850..4969356 (+)	507	WP_032694394.1	type II secretion system minor pseudopilin GspH	-
MMY94_RS23770 (MMY94_23770)	gspI	4969353..4969718 (+)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
MMY94_RS23775 (MMY94_23775)	gspJ	4969715..4970311 (+)	597	WP_032694395.1	type II secretion system minor pseudopilin GspJ	-
MMY94_RS23780 (MMY94_23780)	gspK	4970308..4971288 (+)	981	WP_014227974.1	type II secretion system minor pseudopilin GspK	-
MMY94_RS23785 (MMY94_23785)	gspL	4971281..4972474 (+)	1194	WP_032694396.1	type II secretion system protein GspL	-
MMY94_RS23790 (MMY94_23790)	gspM	4972467..4972952 (+)	486	WP_032694397.1	type II secretion system protein GspM	-
MMY94_RS23795 (MMY94_23795)	-	4972942..4973700 (+)	759	WP_032694398.1	type II secretion system protein N	-
MMY94_RS23800 (MMY94_23800)	pilD	4973703..4974500 (+)	798	WP_032694399.1	prepilin peptidase	Machinery gene
MMY94_RS23805 (MMY94_23805)	hrpB	4974598..4977027 (-)	2430	WP_064388485.1	ATP-dependent helicase HrpB	-
MMY94_RS23810 (MMY94_23810)	thpR	4977100..4977636 (+)	537	WP_032694401.1	RNA 2',3'-cyclic phosphodiesterase	-
MMY94_RS23815 (MMY94_23815)	sfsA	4977636..4978352 (+)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
MMY94_RS23820 (MMY94_23820)	dksA	4978517..4978972 (+)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29150.77 Da
• Isoelectric Point: 8.3410

>NTDB_id=661281 MMY94_RS23800 WP_032694399.1 4973703..4974500(+) (pilD) [Klebsiella michiganensis strain K342]
MENIALLPEFAAQFPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=661281 MMY94_RS23800 WP_032694399.1 4973703..4974500(+) (pilD) [Klebsiella michiganensis strain K342]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATTCCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTAAATCTCTGCC
TCCCCGCCTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGTCTGGTCTGGACGCTGCTGCAGCGTCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A2J5PD60

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.808	98.491	0.491
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.273	100	0.449
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434