Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MJ905_RS21480 Genome accession   NZ_CP092828
Coordinates   4590307..4591149 (+) Length   280 a.a.
NCBI ID   WP_119834446.1    Uniprot ID   A0AB35Q1H5
Organism   Klebsiella michiganensis strain HOBD-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4574064..4590245 4590307..4591149 flank 62


Gene organization within MGE regions


Location: 4574064..4591149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MJ905_RS21400 (MJ905_21395) - 4574552..4575352 (+) 801 WP_014228290.1 PTS mannose/fructose/sorbose transporter subunit IIC -
  MJ905_RS21405 (MJ905_21400) - 4575364..4576212 (+) 849 WP_014228291.1 mannose/fructose/sorbose PTS transporter subunit IID -
  MJ905_RS27790 - 4576344..4579031 (+) 2688 WP_038424193.1 glycoside hydrolase family 32 protein -
  MJ905_RS21420 (MJ905_21415) gspC 4579312..4580169 (+) 858 WP_128696797.1 type II secretion system protein GspC -
  MJ905_RS21425 (MJ905_21420) gspD 4580179..4582149 (+) 1971 WP_255850736.1 type II secretion system secretin GspD -
  MJ905_RS21430 (MJ905_21425) gspE 4582149..4583654 (+) 1506 WP_255850401.1 type II secretion system ATPase GspE -
  MJ905_RS21435 (MJ905_21430) gspF 4583659..4584879 (+) 1221 WP_128696799.1 type II secretion system inner membrane protein GspF -
  MJ905_RS21440 (MJ905_21435) gspG 4584909..4585337 (+) 429 WP_042944533.1 type II secretion system major pseudopilin GspG -
  MJ905_RS21445 (MJ905_21440) gspH 4585337..4585888 (+) 552 WP_119834450.1 type II secretion system minor pseudopilin GspH -
  MJ905_RS21450 (MJ905_21445) gspI 4585885..4586250 (+) 366 WP_119834449.1 type II secretion system minor pseudopilin GspI -
  MJ905_RS21455 (MJ905_21450) gspJ 4586247..4586846 (+) 600 WP_014228301.1 type II secretion system minor pseudopilin GspJ -
  MJ905_RS21460 (MJ905_21455) gspK 4586843..4587829 (+) 987 WP_042944535.1 type II secretion system minor pseudopilin GspK -
  MJ905_RS21465 (MJ905_21460) gspL 4587829..4589034 (+) 1206 WP_128696800.1 type II secretion system protein GspL -
  MJ905_RS21470 (MJ905_21465) gspM 4589021..4589515 (+) 495 WP_014228304.1 type II secretion system protein GspM -
  MJ905_RS21475 (MJ905_21470) - 4589505..4590245 (+) 741 WP_128696801.1 type II secretion system protein N -
  MJ905_RS21480 (MJ905_21475) pilD 4590307..4591149 (+) 843 WP_119834446.1 A24 family peptidase Machinery gene

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 30587.69 Da        Isoelectric Point: 8.8641

>NTDB_id=660564 MJ905_RS21480 WP_119834446.1 4590307..4591149(+) (pilD) [Klebsiella michiganensis strain HOBD-7]
MTLLVAFSSTYPIIWWFGLGATGAIVGSFLNVVICRLPVMLEKHWQREALLQLSLPSPEPTARFNLVLPHSRCPYCHTPV
AARDNIPLLSFLLLKGKTRCCGAPISAQYPLVEAATAALFVLTARVFPPGLALCGAWVFVSFLLILAVIDYHRQLLPDLL
TLPLLWIGLLFNLQSYFVSLPQATIGAVAGYLCLWCLFWLFKLLTGKDALGYGDFKLLAALGAWLGWQALPHVVLLAALS
GLLFAFVQRGGAKKDLNQPLAFGPWLALAGVANMLLFQGI

Nucleotide


Download         Length: 843 bp        

>NTDB_id=660564 MJ905_RS21480 WP_119834446.1 4590307..4591149(+) (pilD) [Klebsiella michiganensis strain HOBD-7]
ATGACATTATTAGTGGCTTTTTCATCCACGTATCCCATCATTTGGTGGTTTGGTCTTGGGGCGACAGGCGCTATCGTCGG
GAGTTTTCTGAATGTGGTGATCTGCCGCTTACCCGTAATGCTCGAGAAACACTGGCAGCGTGAGGCGTTGCTGCAGCTGT
CACTGCCTTCACCTGAACCCACGGCGCGCTTTAATCTGGTATTGCCGCACTCTCGTTGCCCATATTGTCATACTCCTGTT
GCCGCAAGGGATAATATACCTCTGCTGAGTTTCCTGTTGTTAAAGGGCAAGACTCGCTGTTGCGGCGCGCCTATTTCCGC
GCAGTATCCGCTGGTTGAGGCGGCCACCGCTGCGCTGTTTGTCTTAACCGCACGTGTATTTCCGCCGGGGCTGGCGCTGT
GTGGCGCCTGGGTATTCGTCTCTTTTTTATTGATCCTGGCCGTTATTGATTATCACCGACAGCTGCTACCCGATCTCCTG
ACGCTGCCCTTATTGTGGATCGGCTTACTGTTTAATCTTCAGTCGTATTTTGTTTCGTTGCCGCAGGCGACGATTGGCGC
AGTGGCAGGATATCTCTGCTTGTGGTGCCTGTTCTGGCTGTTCAAGCTACTGACAGGCAAGGATGCGCTGGGCTATGGCG
ATTTTAAGCTACTGGCCGCGTTGGGAGCCTGGCTAGGCTGGCAAGCATTACCCCATGTGGTTTTACTGGCGGCGTTAAGC
GGATTGCTGTTTGCGTTCGTACAGCGAGGAGGGGCAAAAAAAGATCTCAATCAGCCGCTGGCATTCGGCCCGTGGCTGGC
GTTAGCCGGTGTGGCAAATATGCTGCTATTTCAGGGTATTTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(137-245)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-124)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.06

95.357

0.496
  pilD Vibrio campbellii strain DS40M4

49.077

96.786

0.475
  pilD Neisseria gonorrhoeae MS11

48.193

88.929

0.429
  pilD Acinetobacter nosocomialis M2

43.902

87.857

0.386
  pilD Acinetobacter baumannii D1279779

43.496

87.857

0.382