Detailed information NTG0660561

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MJ905_RS18950
• Genome accession: NZ_CP092828
• Coordinates: 4040403..4041200 (-)
• Length: 265 a.a.
• NCBI ID: WP_014227970.1
• Uniprot ID: A0A0H3H8P5
• Organism: Klebsiella michiganensis strain HOBD-7
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4035403..4046200

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJ905_RS18930 (MJ905_18925)	dksA	4035931..4036386 (-)	456	WP_004098689.1	RNA polymerase-binding protein DksA	-
MJ905_RS18935 (MJ905_18930)	sfsA	4036551..4037267 (-)	717	WP_014227967.1	DNA/RNA nuclease SfsA	-
MJ905_RS18940 (MJ905_18935)	thpR	4037267..4037803 (-)	537	WP_014837425.1	RNA 2',3'-cyclic phosphodiesterase	-
MJ905_RS18945 (MJ905_18940)	hrpB	4037876..4040305 (+)	2430	WP_255850360.1	ATP-dependent helicase HrpB	-
MJ905_RS18950 (MJ905_18945)	pilD	4040403..4041200 (-)	798	WP_014227970.1	A24 family peptidase	Machinery gene
MJ905_RS18955 (MJ905_18950)	-	4041203..4041961 (-)	759	WP_154925347.1	type II secretion system protein N	-
MJ905_RS18960 (MJ905_18955)	gspM	4041951..4042436 (-)	486	WP_032694397.1	type II secretion system protein GspM	-
MJ905_RS18965 (MJ905_18960)	gspL	4042429..4043622 (-)	1194	WP_032748033.1	type II secretion system protein GspL	-
MJ905_RS18970 (MJ905_18965)	gspK	4043615..4044595 (-)	981	WP_032720565.1	type II secretion system minor pseudopilin GspK	-
MJ905_RS18975 (MJ905_18970)	gspJ	4044592..4045188 (-)	597	WP_032694395.1	type II secretion system minor pseudopilin GspJ	-
MJ905_RS18980 (MJ905_18975)	gspI	4045185..4045550 (-)	366	WP_009654631.1	type II secretion system minor pseudopilin GspI	-
MJ905_RS18985 (MJ905_18980)	gspH	4045547..4046059 (-)	513	WP_042944313.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 265 a.a.
• Molecular weight: 29166.77 Da
• Isoelectric Point: 8.3358

>NTDB_id=660561 MJ905_RS18950 WP_014227970.1 4040403..4041200(-) (pilD) [Klebsiella michiganensis strain HOBD-7]
MENIALLPEFAAQYPSIWGGFLFLSGLAFGSFFNVVIHRLPLMMERAEGVNLCLPASFCPQCRQPIAWRDNIPLLSFLWL
RGRARCCGQPISRRYPLMELTTGALFVLAGYLMAPGMPLLGGLIFVSVLLILAAIDAQTQLLPDRLTLPLLWAGLLFNLS
DTFAPLAEAVIGAMVGYLSLWSVYWVFRLLTGKEALGYGDFKLLAALGAWLGWQPLPQTLLLASASGLVWTLLQRLVTRR
SLEQPLAFGPWLALAGGGIFLWSQV

Nucleotide

• Length: 798 bp

>NTDB_id=660561 MJ905_RS18950 WP_014227970.1 4040403..4041200(-) (pilD) [Klebsiella michiganensis strain HOBD-7]
ATGGAAAATATCGCGCTGTTACCCGAGTTCGCCGCGCAATACCCGTCAATCTGGGGCGGCTTTTTATTCCTGTCCGGACT
GGCGTTCGGCAGCTTTTTTAACGTCGTTATCCATCGTTTACCGCTGATGATGGAACGCGCAGAGGGGGTGAATCTCTGCC
TCCCCGCGTCGTTCTGCCCGCAGTGCCGTCAGCCTATCGCCTGGCGGGACAATATCCCGCTGCTGAGCTTTTTATGGCTC
AGGGGGCGCGCGCGCTGCTGCGGGCAGCCGATCTCGCGTCGCTATCCCCTGATGGAGCTGACGACCGGCGCGCTGTTTGT
ACTCGCGGGGTACCTGATGGCTCCGGGCATGCCGCTGCTGGGCGGGCTAATTTTCGTGTCCGTACTGCTGATCCTCGCCG
CCATCGACGCGCAAACGCAGCTGCTGCCTGACAGGCTGACGCTGCCGCTGCTGTGGGCCGGGCTGCTGTTCAATCTTTCC
GATACCTTCGCGCCGCTGGCGGAGGCGGTCATTGGCGCGATGGTCGGGTATTTGTCGCTGTGGTCGGTGTACTGGGTGTT
CCGTCTGCTGACCGGTAAAGAGGCGCTGGGCTACGGCGATTTCAAGCTGCTGGCAGCGCTCGGCGCGTGGCTCGGCTGGC
AGCCGCTGCCGCAGACGCTGCTGCTGGCTTCCGCCAGCGGCCTGGTCTGGACGCTGCTGCAGCGCCTGGTAACCCGTCGT
TCGCTCGAACAACCTCTGGCCTTCGGCCCCTGGCTGGCGCTGGCGGGCGGCGGCATCTTTTTGTGGAGCCAGGTATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-110)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

3D structure

• AlphaFold DB: A0A0H3H8P5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.708	100	0.498
pilD	Neisseria gonorrhoeae MS11	44.604	100	0.468
pilD	Vibrio campbellii strain DS40M4	43.636	100	0.453
pilD	Acinetobacter nosocomialis M2	44.788	97.736	0.438
pilD	Acinetobacter baumannii D1279779	44.402	97.736	0.434