Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   MK848_RS15430 Genome accession   NZ_CP092824
Coordinates   2999978..3000427 (-) Length   149 a.a.
NCBI ID   WP_003246436.1    Uniprot ID   A0AAE2SFQ0
Organism   Bacillus subtilis strain S1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2994978..3005427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MK848_RS15395 (MK848_15375) yckA 2995250..2995930 (-) 681 WP_024572176.1 amino acid ABC transporter permease -
  MK848_RS15400 (MK848_15380) yckB 2995940..2996803 (-) 864 WP_009966510.1 transporter substrate-binding domain-containing protein -
  MK848_RS15405 (MK848_15385) - 2996965..2997161 (+) 197 Protein_2994 hypothetical protein -
  MK848_RS15410 (MK848_15390) - 2997168..2997515 (+) 348 WP_101172120.1 RDD family protein -
  MK848_RS15415 (MK848_15395) yckD 2997597..2997929 (+) 333 WP_003246247.1 YckD family protein -
  MK848_RS15420 (MK848_15400) bglC 2998083..2999516 (+) 1434 WP_003246242.1 6-phospho-beta-glucosidase -
  MK848_RS15425 (MK848_15405) nin/comJ 2999553..2999951 (-) 399 WP_003246342.1 DNA-entry nuclease inhibitor Regulator
  MK848_RS15430 (MK848_15410) nucA/comI 2999978..3000427 (-) 450 WP_003246436.1 DNA-entry nuclease Machinery gene
  MK848_RS15435 (MK848_15415) tlpC 3000595..3002316 (-) 1722 WP_003246498.1 methyl-accepting chemotaxis protein TlpC -
  MK848_RS15440 (MK848_15420) hxlB 3002427..3002984 (-) 558 WP_003246343.1 6-phospho-3-hexuloisomerase -
  MK848_RS15445 (MK848_15425) hxlA 3002990..3003622 (-) 633 WP_003246452.1 3-hexulose-6-phosphate synthase -
  MK848_RS15450 (MK848_15430) hxlR 3003855..3004217 (+) 363 WP_003246265.1 transcriptional activator HxlR -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16486.48 Da        Isoelectric Point: 4.6274

>NTDB_id=660453 MK848_RS15430 WP_003246436.1 2999978..3000427(-) (nucA/comI) [Bacillus subtilis strain S1]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETMAFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHRLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=660453 MK848_RS15430 WP_003246436.1 2999978..3000427(-) (nucA/comI) [Bacillus subtilis strain S1]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATGGCCTTCCCATCTGACCGGT
ATCCCGAAACTGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATTGACAGAGACGGAGCT
GAAGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
GGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

100

100

1