NTDB

Overview

Name	pilD	Type	Machinery gene
Locus tag	MJ904_RS12025	Genome accession	NZ_CP092780
Coordinates	2627509..2628378 (-)	Length	289 a.a.
NCBI ID	WP_050409291.1	Uniprot ID	A0A0K1JZ69
Organism	Massilia sp. MB5
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2622509..2633378

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MJ904_RS11995 (MJ904_11995)	argJ	2623182..2624420 (+)	1239	WP_240737533.1	bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ	-
MJ904_RS12000 (MJ904_12000)	-	2624424..2625305 (+)	882	WP_240737534.1	ATP-binding protein	-
MJ904_RS12005 (MJ904_12005)	-	2625302..2625705 (+)	404	Protein_2370	NUDIX domain-containing protein	-
MJ904_RS12010 (MJ904_12010)	yacG	2625810..2625995 (-)	186	WP_050409294.1	DNA gyrase inhibitor YacG	-
MJ904_RS12015 (MJ904_12015)	zapD	2626008..2626763 (-)	756	WP_050409293.1	cell division protein ZapD	-
MJ904_RS12020 (MJ904_12020)	coaE	2626828..2627478 (-)	651	WP_240737535.1	dephospho-CoA kinase	-
MJ904_RS12025 (MJ904_12025)	pilD	2627509..2628378 (-)	870	WP_050409291.1	A24 family peptidase	Machinery gene
MJ904_RS12030 (MJ904_12030)	-	2628406..2629622 (-)	1217	Protein_2375	type II secretion system F family protein	-
MJ904_RS12035 (MJ904_12035)	pilB	2629679..2631408 (-)	1730	Protein_2376	type IV-A pilus assembly ATPase PilB	-
MJ904_RS12040 (MJ904_12040)	-	2631503..2632792 (-)	1290	WP_050409288.1	HlyC/CorC family transporter	-

Sequence

Protein

Download Length: 289 a.a. Molecular weight: 31016.78 Da Isoelectric Point: 8.3290

                                >NTDB_id=660283 MJ904_RS12025 WP_050409291.1 2627509..2628378(-) (pilD) [Massilia sp. MB5]

MPEYLMLFSPPGTPGAAIIAGIFGLMIGSFLNVVIYRLPKMMQRESDNYVAAESGKQLPHTDRFNLMVPRSACPHCGHQV

TALENVPVFSWLALRGKCSGCKNPISARYPLIEALTALLSAGLVWQFGSGYMGLATLVFAWMLIAMTFIDADTQLLPDDL

TYPLLWAGLLVNLNGTFVPLRDAVIGAAAGYLALWSIFWLFKLATGKEGMGYGDFKLLAALGAWLGWAMLPSIILLSSVV

GAVVGICLIVFARHGRNNPIPFGPYLAAAGMIALIFGAPISRFTSSLAG

Nucleotide

Download Length: 870 bp

                                >NTDB_id=660283 MJ904_RS12025 WP_050409291.1 2627509..2628378(-) (pilD) [Massilia sp. MB5]

ATGCCTGAATACCTCATGCTGTTTTCGCCGCCCGGCACGCCGGGCGCCGCCATCATCGCCGGCATTTTCGGCCTGATGAT

AGGCAGTTTCCTGAACGTGGTGATCTACCGCCTGCCGAAGATGATGCAGCGCGAGTCGGACAATTATGTGGCAGCCGAAT

CGGGCAAGCAGCTGCCGCATACCGACCGCTTCAACCTGATGGTGCCGCGTTCGGCCTGCCCGCACTGCGGCCACCAGGTG

ACGGCACTGGAAAACGTCCCCGTGTTCAGCTGGCTGGCCCTGCGCGGCAAATGCAGCGGCTGCAAGAACCCGATCTCGGC

GCGCTATCCGCTGATCGAGGCCTTGACCGCCCTGCTGTCGGCGGGCCTGGTGTGGCAATTCGGCAGCGGCTATATGGGCC

TGGCCACCCTGGTCTTCGCCTGGATGCTGATCGCCATGACCTTCATCGACGCCGATACCCAGCTGCTGCCGGACGACCTG

ACCTACCCGCTGCTGTGGGCCGGCCTGCTGGTGAACCTGAACGGCACCTTCGTGCCGCTGCGCGATGCCGTGATCGGCGC

CGCCGCCGGCTATCTGGCGCTATGGAGCATCTTCTGGCTCTTCAAGCTGGCGACGGGCAAGGAGGGAATGGGGTATGGTG

ACTTCAAGCTGCTGGCGGCGCTGGGCGCCTGGCTGGGCTGGGCCATGCTGCCATCGATCATCCTGCTGTCCTCGGTGGTG

GGCGCGGTGGTCGGCATCTGCCTGATCGTCTTTGCGCGGCACGGCCGCAACAACCCGATTCCCTTTGGTCCTTATCTGGC

GGCGGCCGGCATGATCGCCCTGATCTTCGGCGCGCCGATCAGCCGTTTCACCAGCAGCCTGGCGGGCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(22-127)

Type IV leader peptidase family (Peptidase_A24)

(137-246)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0K1JZ69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilD	Vibrio cholerae strain A1552	57.895	92.042	0.533
pilD	Vibrio campbellii strain DS40M4	55.797	95.502	0.533
pilD	Neisseria gonorrhoeae MS11	50	91.349	0.457
pilD	Acinetobacter baumannii D1279779	49.438	92.388	0.457
pilD	Acinetobacter nosocomialis M2	48.315	92.388	0.446

Detailed information NTG0660283