Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   MKF36_RS12205 Genome accession   NZ_CP092750
Coordinates   2486653..2487267 (-) Length   204 a.a.
NCBI ID   WP_007408263.1    Uniprot ID   -
Organism   Bacillus velezensis strain KS04AU     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2481653..2492267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF36_RS12175 (MKF36_12175) rpsT 2481956..2482222 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  MKF36_RS12180 (MKF36_12180) holA 2482239..2483280 (-) 1042 Protein_2412 DNA polymerase III subunit delta -
  MKF36_RS12185 (MKF36_12185) - 2483338..2483471 (-) 134 Protein_2413 hypothetical protein -
  MKF36_RS12190 (MKF36_12190) - 2483512..2483646 (+) 135 WP_003152870.1 YqzM family protein -
  MKF36_RS12195 (MKF36_12195) comEC 2483665..2486016 (-) 2352 WP_007408265.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  MKF36_RS12200 (MKF36_12200) - 2486017..2486586 (-) 570 WP_007408264.1 ComE operon protein 2 -
  MKF36_RS12205 (MKF36_12205) comEA 2486653..2487267 (-) 615 WP_007408263.1 helix-hairpin-helix domain-containing protein Machinery gene
  MKF36_RS12210 (MKF36_12210) comER 2487326..2488147 (+) 822 WP_007408262.1 late competence protein ComER -
  MKF36_RS12215 (MKF36_12215) - 2488216..2488953 (-) 738 WP_015417856.1 class I SAM-dependent methyltransferase -
  MKF36_RS12220 (MKF36_12220) rsfS 2488950..2489306 (-) 357 WP_007408260.1 ribosome silencing factor -
  MKF36_RS12225 (MKF36_12225) yqeK 2489323..2489883 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  MKF36_RS12230 (MKF36_12230) - 2489873..2490442 (-) 570 WP_042635364.1 nicotinate-nucleotide adenylyltransferase -
  MKF36_RS12235 (MKF36_12235) yhbY 2490453..2490743 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  MKF36_RS12240 (MKF36_12240) aroE 2490737..2491579 (-) 843 WP_007408256.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22223.39 Da        Isoelectric Point: 6.4940

>NTDB_id=660036 MKF36_RS12205 WP_007408263.1 2486653..2487267(-) (comEA) [Bacillus velezensis strain KS04AU]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=660036 MKF36_RS12205 WP_007408263.1 2486653..2487267(-) (comEA) [Bacillus velezensis strain KS04AU]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCTTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.333

94.118

0.549
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363